Studies on cell wall regeneration in protoplast culture of legumes - the effect of organic medium additives on cell wall components

Wiszniewska, Alina; Piwowarczyk, Barbara

doi:10.17221/108/2013-cjgpb

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…Wiszniewska and Piwowarczyk () studied the cell wall regeneration in mesophyll protoplasts of yellow lupin and grass pea analysing the occurrence of cell wall components: cellulose, callose and arabinogalactan proteins during 15 days of culture in different media. Medium supplementation with 2 mg/L chitosan resulted in prolonged viability, more balanced cellulose resynthesis, increased callose formation and induction of mitotic divisions in protoplast‐derived cells of both examined legumes.…”

Section: Distant Gene Transfermentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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Grain legumes, the important constituents of sustainability-based cropping systems and energy-limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so-called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest.The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement.

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Section: Distant Gene Transfermentioning

confidence: 99%

Potential, constraints and applications of in vitro methods in improving grain legumes

et al. 2018

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“…Cell wall composition is also an important factor controlling plant regeneration from protoplast cultures [19,22,29]. It is postulated that the cell wall reconstruction is a key step in reprogramming and dedifferentiation in protoplast cultures [19,41]. Diverse cell wall constituencies are postulated to regulate the de- and redifferentiation processes during protoplast wall re-synthesis.…”

Section: Discussionmentioning

confidence: 99%

Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus Is Affected by Phytosulfokine (PSK)

Godel-Jędrychowska

Maćkowska

Kurczyńska

et al. 2019

IJMS

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Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation. It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation. Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process. So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells. Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture. Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration. Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used. The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.

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“…Cultured protoplast is one of the suitable materials to allow investigation of the dynamics of primary cell wall formation, e.g., arrangements of cellulose microfibrils and the sequence of polysaccharide synthesis, as well as compositional changes in cell walls during cell division [11][12][13][14][15][16][17]. Protoplasts, which lack cell walls, are appropriate for investigation of primary wall formation from initiation to the final stages.…”

Section: Introductionmentioning

confidence: 99%

“…Previous reports have investigated cell wall formation by protoplasts of herbaceous plants [11,[14][15][16][17]24], but few comparable studies have been undertaken on protoplasts of woody plants [12], which are important for biomass utilization. Therefore, the current study aimed to clarify the occurrence of cellulose microfibrils (CMFs) in protoplasts of white birch callus under the stress culture condition by in situ observation.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration

et al. 2019

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In this study, dynamic changes in structural polysaccharide deposition on the plasma membrane and cortical microtubules (CMTs) behavior were monitored in protoplasts isolated from white birch callus using confocal laser scanning microscopy and atomic force microscopy. We focused on the influence of an environmental stimulus on cell wall regeneration in protoplasts by employing an acidic culture medium containing a high concentration of Ca2+ (the stress condition). Under the non-stress condition, cellulose microfibrils and callose were initially synthesized, and thereafter deposited on the plasma membrane as “primary cell wall material”. Under the stress condition, callose micro-sized fibers were secreted without cell wall regeneration. Behavior of CMTs labeled with mammalian microtubule-associated protein 4 with green fluorescent protein in transgenic protoplasts was monitored by time-lapse video analysis. Under the non-stress condition, CMTs behavior showed a linear arrangement at a fixed position, whereas unfixed manner of CMTs behavior was observed under the stress condition. These findings indicate that excessive Ca2+ affects cellulose synthesis and CMTs dynamics in plant protoplasts. Current study first demonstrated dynamics of cell wall regeneration and CMTs in woody protoplast, which provides novel insight to aid in understanding early stages of primary cell wall formation in plants.

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Studies on cell wall regeneration in protoplast culture of legumes - the effect of organic medium additives on cell wall components

Cited by 12 publications

References 24 publications

Potential, constraints and applications of in vitro methods in improving grain legumes

Potential, constraints and applications of in vitro methods in improving grain legumes

Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus Is Affected by Phytosulfokine (PSK)

Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration

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