Studies on carotenoid-containing lipoproteins isolated from the eggs and ovaries of certain marine invertebrates

Zagalsky, P.F.; Cheesman, D. F.; Ceccaldi, Hubert J.

doi:10.1016/0010-406x(67)90777-3

Cited by 87 publications

(15 citation statements)

References 45 publications

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“…The pellets of 60% saturated ammonium sulphate solution were suspended in appropriate volume of homogenization buffer and dialyzed thrice at 4°C for 12 h each against homogenization buffer. Further, the isolated vitellogenin and vitellin were purified by following the scheme of Zagalsky et al [36]. Then the purified vitellogenin and vitellin were stored at −20°C until further analysis.…”

Section: Methodsmentioning

confidence: 99%

TBT Effects on the Development of Intersex (Ovotestis) in Female Fresh Water PrawnMacrobrachium rosenbergii

Revathi

Iyapparaj

Lourdaraj

et al. 2014

BioMed Research International

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The impact of tributyltin (TBT) on the female gonad and the endocrine system in Macrobrachium rosenbergii was studied. Prawns were exposed to environmentally realistic concentrations of 10, 100, and 1000 ng/L of TBT for 6 months. Dose dependent effects were noticed in TBT exposed prawns. At 1000 ng/L TBT caused ovotestis formation (formation of male germ cells in ovary). Presence immature oocytes, fusion of developing oocytes, increase in interstitial connective tissues, and its modification into tubular like structure and abundance of spermatogonia in the ovary of TBT treated prawns. The control prawn ovary showed normal architecture of cellular organelles such as mature oocytes with type 2 yolk globules, lipid droplets, normal appearance of yolk envelop, and uniformly arranged microvilli. On the other hand, type 1 yolk globules, reduced size of microvilli, spermatogonial cells in ovary, spermatogonia with centrally located nucleus, and chromatin distribution throughout the nucleoplasm were present in the TBT treated group. Immunofluorescence staining indicated a reduction in vitellin content in ovary of TBT treated prawn. Moreover, TBT had inhibited the vitellogenesis by causing hormonal imbalance in M. rosenbergii. Thus, the present investigation demonstrates that TBT substantially affects sexual differentiation and gonadal development in M. rosenbergii.

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Section: Methodsmentioning

confidence: 99%

TBT Effects on the Development of Intersex (Ovotestis) in Female Fresh Water PrawnMacrobrachium rosenbergii

Revathi

Iyapparaj

Lourdaraj

et al. 2014

BioMed Research International

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show abstract

“…The pellets of 60% saturated ammonium sulphate solution was suspended in appropriate volume of homogenization buffer and dialyzed thrice at 4°C for 12h each against homogenization buffer. Further, the isolated vitellogenin and vitellin were purified by following the scheme of Zagalsky et al [43]. Then the purified vitellogenin and vitellin were stored at -20°C for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Impact of TBT on the vitellogenesis and sex hormones in freshwater prawn Macrobrachium rosenbergii (De Man, 1879)

et al. 2013

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BackgroundTributyltin (TBT) is a ubiquitous persistent xenobiotic that can be found in freshwater, estuarine and marine ecosystem. TBT is a strong endocrine disrupting compound (EDC) that can cause toxic threat to aquatic organisms. Imposex, sexual deformities and endocrine dysfunctions are the causes of TBT to most of the aquatic organisms. Effect of TBT on the vitellogenesis and sex hormonal changes in Macrobrachium rosenbergii has never been reported. Hence, the present investigation was undertaken to find out the impact of TBT on histological changes in the different reproductive tissues, sex hormonal alterations and level of biomarkers like vitellogenin and vitellin in M. rosenbergii.ResultsThe present investigation documents the possible impact of tributyltin (TBT) on the vitellogenesis in freshwater female prawn M. rosenbergii. TBT at 10 ng/l, 100 ng/l and 1000 ng/l concentrations were exposed individually to prawns for a period of three months. At higher concentration of 1000 ng/l, the ovarian development was arrested and ovary remained at spent stage. At lower concentration of TBT (10 ng/l), the development proceeded up to early vitellogenic stage. At intermediate concentration of 100 ng/l TBT, the ovary remained at pre vitellogenic stage and thereafter no development was noticed. Histological results indicated the normal ovarian development with vitellogenic oocytes, filled with yolk globules in control prawn. On the other hand, the TBT treated groups showed reduction in yolk globules, fusion of developing oocytes and abundance of immature oocytes. Immunofluorescence staining denoted the remarkable reduction in vitellin content in ovary of TBT treated prawn. Hence, TBT had conspicuously inhibited the vitellogenesis by causing hormonal imbalance in M. rosenbergii.ConclusionTBT had notably inhibited the vitellogenesis due to hormonal imbalance. This endocrine dysfunction ultimately impaired the oogenesis in the freshwater female prawn M. rosenbergii.

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“…But efforts have mostly been focused on determination of their components and moiety in the past. Evidence suggests that yolk proteins contain carbohydrates, phospholipids, and carotenoid components (Zagalsky et al, 1967;Kerr, 1969;Fyffe and O'Connor, 1974; de Chaffoy de 'Corresponding author. Courcelles and Kondo, 1980;Chen et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Hepatopancreas and ovary are sites of vitellogenin synthesis as determined from partial cDNA encoding of vitellogenin in the marine shrimp,Penaeus vannamei

Tseng

Chen

Liu³

et al. 2002

Invertebrate Reproduction & Development

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Studies on carotenoid-containing lipoproteins isolated from the eggs and ovaries of certain marine invertebrates

Cited by 87 publications

References 45 publications

TBT Effects on the Development of Intersex (Ovotestis) in Female Fresh Water PrawnMacrobrachium rosenbergii

TBT Effects on the Development of Intersex (Ovotestis) in Female Fresh Water PrawnMacrobrachium rosenbergii

Impact of TBT on the vitellogenesis and sex hormones in freshwater prawn Macrobrachium rosenbergii (De Man, 1879)

Hepatopancreas and ovary are sites of vitellogenin synthesis as determined from partial cDNA encoding of vitellogenin in the marine shrimp,Penaeus vannamei

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