Studies on Canine Parvovirus Isolation, Experimental Infection and Serologic Survey

Azetaka, Masayuki; Hirasawa, Tsutomu; Konishi, Seiki; Ogata, Manabu

doi:10.1292/jvms1939.43.243

Cited by 43 publications

(26 citation statements)

References 16 publications

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“…A chronological table of the epidemic strain of CPV in Japan was made based on the present result together with the previous findings [2,11,12,15,27,29,30,32,35]. As shown in Table 3, CPV-2 first appeared at the beginning of 1979 in Tokyo [2] and existed till 1983 in the field.…”

Section: Discussionsupporting

confidence: 54%

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

et al. 2008

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ABSTRACT. Fifty-five canine parvovirus type 2 (CPV) samples, 12 fecal specimens and 43 cell culture isolates, were examined for their genetic characteristics of VP2 gene. They were collected from the diseased dogs at various districts of Japan during 27 years from 1980 to 2006. A fragment of VP2 gene was analyzed by restriction fragment length polymorphism assay and DNA sequencing. The original antigenic type 2 of CPV (CPV-2) was no longer found in the samples since 1984, and two antigenic variants CPV-2a and CPV-2b replaced CPV-2 as predominant types for about 5 years from 1982. A new genetic variant of prototype CPV-2a with non-synonymous substitution at the VP2 amino acid residue 297 from Ser to Ala was first detected in 1987. New CPV-2b with the same amino acid substitution at position 297 as new CPV-2a was also detected from the samples collected in 1997. Since then new CPV-2b has been the predominant CPV over the field of Japan. Several additional amino acid substitutions were detected in the VP2 gene of some recent CPV strains. Neither CPV-2c(a), CPV-2c(b), nor "Glu-426" of the antigenic variants previously found outside the country was detected in any samples tested. Reactivity of new CPV-2a and 2b variants against antibodies produced by the current vaccine products was determined by a cross hemagglutination-inhibition test. The recent field CPV isolates reacted more efficiently to the antibodies produced in dogs vaccinated with the new CPV-2b vaccine strain than the conventional CPV-2 vaccine strain.

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Section: Discussionsupporting

confidence: 54%

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

et al. 2008

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“…Antigenically the virus is closely related to feline parvovirus (FPV) (Johnson & Spradbrow, 1979;Carmichael et al, 1980;Lenghaus & Studdert, 1980) of which two variant viruses, feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), are known (Siegl, 1976). The first outbreaks of CPV disease were observed in 1978 when epidemics occurred almost simultaneously in all five continents (Kelly, 1978;Appel et al, 1979;Burtonboy et al, 1979;Van Rensburg et al, 1979;Azetaka et al, 1981). In dog sera collected before 1976 no antibodies against CPV have been found (Carmichael & Binn, 1981).…”

Section: Introductionmentioning

confidence: 99%

Canine Parvovirus: Relationship to Wild-type and Vaccine Strains of Feline Panleukopenia Virus and Mink Enteritis Virus

Tratschin

McMaster²,

Kronauer

et al. 1982

Journal of General Virology

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SUMMARYCanine parvovirus (CPV), feline panleukopenia virus (FPLV) and mink enteritis virus (MEV) were compared serologically, by determination of their host range in cell cultures, as well as by restriction enzyme analysis. Maps of the virus genomes were established using seven different restriction enzymes cutting at a total of 56 sites. MEV and FPLV gave maps which were identical except for one restriction site. The map of CPV is closely related to those of FPLV/MEV since their DNAs share about 80% of the restriction sites tested. However, CPV is clearly distinct from FPLV/MEV since either eight (German isolate) or nine (Belgian, Swiss and American isolates) restriction sites are different. The DNAs of six vaccine strains of FPLV and MEV were also analysed. They gave maps which closely resembled those of the respective wild-type strains. CPV and FPLV/MEV also differed with respect to antigenicity, as well as to host range in cell cultures.

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“…Sci. 70(12): 1305-1314 Canine parvovirus type 2 (CPV), one of the feline parvovirus (FPV) subspecies, emerged suddenly throughout the world in the late 1970s as a new pathogen that caused severe hemorrhagic gastroenteritis and myocarditis in domestic dogs [1,2,7,20,23,40]. Mortality rate is usually high, particularly in non-immune pups.…”

mentioning

confidence: 99%

Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

et al. 2008

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ABSTRACT. Canine parvovirus type 2 (CPV) is a pathogen that causes severe hemorrhagic gastroenteritis with a high fatality rate in pups worldwide. Since CPV emerged in the late 1970s, its origin has been explored with the conclusion that CPV originated from feline panleukopenia virus or a closely related virus. Both high mutation rate and recombination are assumed to be key factors in the evolution of parvoviruses. Here we provide evidence for natural recombination in CPV isolated from dogs in cell culture. Antigenic and genetic properties of isolates from 10 diseased pups were elucidated. Six pups had been vaccinated beforehand with live combined vaccine containing original antigenic type CPV (CPV-2). Six isolates recovered from 4 vaccinated pups in cell cultures were found to contain either CPV-2 or CPV-2-like viruses. The other isolates, including all those from non-vaccinated pups, were CPV-2b viruses. Antigenic typing of two CPV-2-like isolates, 03-029/M and 1887/f, with a monoclonal antibody panel suggested they were a mixture of CPV-2 and CPV2a (03-029/M) and a recombinant of CPV-2 and CPV-2b (1887/f). Genetic analysis of the VP1 gene indicated that isolate 03-029/M was a mixture of CPV-2, CPV-2a and a recombinant of CPV-2 and CPV-2a viruses, while isolate 1887/f was composed of a recombinant of CPV-2 and CPV-2b viruses. This is the first demonstration of natural CPV recombination in the field and suggests that recombination in the evolution of CPV is a more frequent and important process than previously believed. KEY WORDS: canine parvovirus, cell culture, dog, parvovirus, recombination.J. Vet. Med. Sci. 70(12): 1305-1314 Canine parvovirus type 2 (CPV), one of the feline parvovirus (FPV) subspecies, emerged suddenly throughout the world in the late 1970s as a new pathogen that caused severe hemorrhagic gastroenteritis and myocarditis in domestic dogs [1,2,7,20,23,40]. Mortality rate is usually high, particularly in non-immune pups. The origin of the virus is still not clear but the most likely hypothesis from phylogenetic analysis is that CPV originated from feline panleukopenia virus (FPLV) or a very closely related carnivore parvovirus of feral canids, such as foxes and mink [52,55]. There has been speculation that, during circulation for some time in an European local dog population, this ancestral CPV gradually adapted for domestic dogs and the emergent original CPV (antigenic type 2: CPV-2) rapidly spread globally as a new pathogen of domestic dogs.In the early 1980s, this first CPV-2 disappeared from the field having been replaced by a new antigenic variant designated CPV-2a, and another antigenic variant CPV-2b appeared soon afterwards. These variants can be distinguished by monoclonal antibodies (MAbs) [35,43,[45][46][47]. CPV-2a and 2b use both canine and feline transferrin receptors for binding to cells both in vitro and in vivo [17,42] and consequently can infect dogs as well as cats [34,56]. In contrast, CPV-2 can infect both feline and canine cells in vitro but infects only dogs in vivo [57]...

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Studies on Canine Parvovirus Isolation, Experimental Infection and Serologic Survey

Cited by 43 publications

References 16 publications

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

Canine Parvovirus: Relationship to Wild-type and Vaccine Strains of Feline Panleukopenia Virus and Mink Enteritis Virus

Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

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