Studies on canine bone marrow long-term culture: effect of stem cell factor

Neuner, E.; Schumm, Michael; Schneider, Eva-Maria; Guenther, W.; Kremmer, Elisabeth; Vogl, Christiane; Büttner, M; Thierfelder, S.; Kolb, Hans‐Jochem

doi:10.1016/s0165-2427(97)00126-8

Cited by 4 publications

(6 citation statements)

References 23 publications

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“…The canine bone marrow culture conditions tested in this study are based on our previous experience in murine and canine cell cultures [3, 17]and on other authors’ findings [13, 15, 16]. Although we obtained functional and mature neutrophils before recharging (table 2; fig.…”

Section: Discussionmentioning

confidence: 99%

“…Canine LTBMC were established according to previously reported techniques [13, 15, 16]and personal experience [3, 17]. MNC were obtained by density-gradient centrifugation on Ficoll-Paque TM and cultured in 25-cm 2 tissue culture flasks (10 6 MNC/ml) with 10 ml of Iscove’s modified Dulbecco’s medium (IMDM; Sigma Chemical Co.; 330 mosm) containing 20% allogeneic dog serum (DS), 10 –7 M hydrocortisone sodium hemisuccinate (HC; Sigma Chemical Co.), 1.4 m M glutamine and penicillin/streptomycin (60 U/ml and 60 µg/ml).…”

Section: Methodsmentioning

confidence: 99%

“…In large outbred animals like dogs and monkeys, bone marrow transplantation problems closely resemble those observed in human patients, permitting the successful use in humans of the knowledge obtained from these animal studies [1, 2]. Dogs can be an excellent study model, although to date less is known about the canine hemopoietic system than about those of humans, rats and mice [3]. …”

Section: Introductionmentioning

confidence: 99%

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Canine Long-Term Bone Marrow Culture Neutrophil Production and Functionality

Fermín¹,

Gaitán

Fragío³

et al. 2004

Acta Haematol

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This in vitro study has been conducted to determine the optimal experimental conditions under which to produce canine neutrophils in long-term bone marrow cultures (LTBMC), establish functional parameters of neutrophils obtained from LTBMC and peripheral blood and to ascertain whether these cells display physiological similarities. Our aim is to provide an experimental model, enabling a correlation between hemopoietic injury and neutrophil functionality. The authors demonstrate for the first time that canine neutrophils grown in cultures are able to produce oxyradicals capable of killing bacterial products. Moreover, culture-grown neutrophils contain gelatinase granules, a marker of terminal neutrophil differentiation, and express a specific surface antigen. The results described in this article illustrate the development of a dynamic system that mimics physiological hemopoiesis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Canine Long-Term Bone Marrow Culture Neutrophil Production and Functionality

Fermín¹,

Gaitán

Fragío³

et al. 2004

Acta Haematol

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“…Stromal cell primary culture: Stromal cells were cultured according to a method previously reported [33]. Briefly, BMMCs were cultured in Iscove's Modified Dulbecco's Medium (IMDM) (Sigma-Aldrich, St. Louis, MO) supplemented with 20% horse serum (Gibco, Invitrogen, Carlsbad, CA) and 10 -7 M of hydrocortisone (Solu-Cortef ® ; Pfizer Japan Inc., Tokyo, Japan).…”

Section: Animals and Cellsmentioning

confidence: 99%

Quantitative Analysis of mRNA Transcripts of Hox, SHH, PTCH, Wnt, and Fzd Genes in Canine Hematopoietic Progenitor Cells and Various in vitro Colonies Differentiated from the Cells

Ide

Goto‐Koshino

Momoi

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Homeobox (Hox), Sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Frizzled (Fzd) and Patched1 (PTCH1) represent the receptors of Wnt and SHH, respectively. In this study, the amounts of mRNA transcripts of the genes associated with the self-renewal of hematopoietic stem cells, HoxB3, HoxB4, HoxA10, Wnt5a, Wnt2b, Fzd1, Fzd6, SHH, and PTCH1, were measured in canine unfractionated bone marrow cells, CD34-enriched cells, and various colony-forming units in culture (CFU-C). Partial cDNA sequences of these 9 canine genes were determined in this study. Quantitative real-time polymerase chain reaction was employed to indicate their relative amounts of mRNA transcripts. Amounts of mRNA transcripts of HoxB3, HoxA10, PTCH1, and Wnt5a genes in canine CD34-enriched cell fraction were significantly larger than those in the CD34-depleted cell fraction. Amounts of mRNA transcripts of HoxB3, HoxA10, PTCH1, Wnt5a, and Wnt2b genes in various CFU-C cells were significantly smaller than those in the seeded CD34-enriched cell fraction. These results suggested important roles of the products of these genes in self-renewal, expansion, and survival of hematopoietic progenitor cells in dogs as shown in humans and rodents.

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“…Após uma centrifugação com o reagente FICOLL as células nucleadas formaram um "anel" na interface, esse "anel" foi retirado e após a centrifugação formou um pellet contendo células mononucleares e algumas hemácias. Igualmente ocorreu em experimento realizado por Al-Lebban, 1987;Neuner, 1998;Guzman, 2002;Bieback, 2004;Jin-Fu Wang, 2004;Lu-Lu Lu, 2006 Com a metodologia empregada, e as concentrações das substâncias utilizadas em nossa tentativa, não obtivemos resultados satisfatórios. As células não apresentaram a marcação desejada através das colorações alizarin red s e oil red o utilizadas para visualização das culturas celulares induzidas para osteogêse e adipogênese.…”

Section: Discussionunclassified

Isolamento, quantificação e caracterização morfológica de células mesenquimais de sangue do cordão umbilical canino

Ozorio¹

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Studies on canine bone marrow long-term culture: effect of stem cell factor

Cited by 4 publications

References 23 publications

Canine Long-Term Bone Marrow Culture Neutrophil Production and Functionality

Canine Long-Term Bone Marrow Culture Neutrophil Production and Functionality

Quantitative Analysis of mRNA Transcripts of Hox, SHH, PTCH, Wnt, and Fzd Genes in Canine Hematopoietic Progenitor Cells and Various in vitro Colonies Differentiated from the Cells

Isolamento, quantificação e caracterização morfológica de células mesenquimais de sangue do cordão umbilical canino

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