Studies on Camel Pancreatic Lipase and Colipase

Abstract

Cysticercosis by Taenia solium is an important disease caused by the presence of larval stage in swine and human, can produce several clinical manifestation including death. In MCxico 10% of medical attention in neurological disease is because cysticercosis. The lack of economical resources and poor hygiene in the communities, difficult the implantation of preventive methods. However molecules capable to induce protection against helminths have been proposed, for example, the Glutathione S-Transferase (GST), the Triose phosphate isomerase (TPI) and the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). These enzymes are vaccine candidates against schistosomiosis. The aim of this study is the partial characterization of TPI and G A P D H of Taenia solium for future use in vaccination assays. cDNA clones of TPI and G A P D H isolated from a library, encodes a protein of 250 and 336 amino acids respectively. Amino acids sequences have a strong homology with others reported enzymes. The Taenia TPI had conserved catalytic site amino acids and astructure characteristic of this enxymes with 8 domains alpha-beta. The cDNAs coding to the enzymes were cloned into the expression vector pRSET to produce the recombinant enzymes. The recombinant TPI and G A P D H shown a molecular mass of 27 kDa and 35 kDa respectively determined by SDS-PAGE. The recombinant TPI has catalytic activity and preliminary vaccination assays with this enzyme promoted 55% of parasite reduction in a cysticercosis murine model.