Studies on Botrytis spp. occurring on onions (<i>Allium cepa</i>) and leeks (<i>Allium porrum</i>)

Presly, A. H.

doi:10.1111/j.1365-3059.1985.tb01382.x

Cited by 23 publications

(14 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However morphological differences seen in Botrytis spp. isolated from a single plant also might be an indication of several different Botrytis species specialized to infect a common host, as seen with neck rot of onion (Allium cepa L.) (Presly 1985).…”

Section: Introductionmentioning

confidence: 99%

DNA sequence analysis of herbarium specimens facilitates the revival of Botrytis mali, a postharvest pathogen of apple

O’Gorman

Sholberg²,

Stokes³

et al. 2008

Mycologia

View full text Add to dashboard Cite

The fungus Botrytis cinerea has been widely accepted as the species responsible for causing gray mold decay of apple, although a second species causing apple decay, B. mali, was reported in 1931. Botrytis mali was validly published in 1931, nevertheless it has always been considered a doubtful species. To study the relationship of Botrytis isolates causing gray mold on apple, DNA sequence analysis was employed. Twenty-eight Botrytis isolates consisting of 10 species were sampled, including two B. mali herbarium specimens from apple originally deposited in 1932. The DNA sequence analysis of the beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) genes placed the isolates into groupings with defined species boundaries that generally reflected the morphologically based model for Botrytis classification. The B. cinerea isolates from apple and other host plants were placed in a single clade. The B. mali herbarium specimens however always fell well outside that clade. The DNA sequence analysis reported in this study support the initial work by Ruehle (1931) describing the apple pathogen B. mali as a unique species.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA sequence analysis of herbarium specimens facilitates the revival of Botrytis mali, a postharvest pathogen of apple

O’Gorman

Sholberg²,

Stokes³

et al. 2008

Mycologia

View full text Add to dashboard Cite

show abstract

“…Until the recent taxonomic and nomenclatural clarification of these neck rot species by Yohalem et al (76), B. aclada was considered by many to be synonymous with B. allii, while B. byssoidea was regarded by some as conspecific with B. aclada (23). The lack of distinction of these species was due, in part, to limitations at differentiating these species using classical morphological and cultural methods (47,48). Nonetheless, Owen et al (42) demonstrated that B. byssoidea and B. aclada are valid species.…”

mentioning

confidence: 97%

“…However, the two species are difficult to distinguish morphologically, with similar growth patterns on agar media and overlapping spore sizes (7, 76). B. byssoidea is occasionally reported as causing neck rot (47,73). However, the true impact of B. byssoidea may be underestimated because of difficulty in isolating and identifying this species, which sporulates sparsely on most common media used to isolate fungi from plant tissues (7).…”

mentioning

confidence: 99%

A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion

Chilvers

Toit²,

Akamatsu

et al. 2007

Plant Disease

View full text Add to dashboard Cite

A real-time fluorescent polymerase chain reaction (PCR) assay was developed using SYBR Green chemistry to quantify the Botrytis spp. associated with onion (Allium cepa) seed that are also able to induce neck rot of onion bulbs, i.e., B. aclada, B. allii, and B. byssoidea. The nuclear ribosomal intergenic spacer (IGS) regions of target and nontarget Botrytis spp. were sequenced, aligned, and used to design a primer pair specific to B. aclada, B. allii, and B. byssoidea. Primers and amplification parameters were optimized to avoid amplifying the related species B. cinerea, B. porri, and B. squamosa, as well as Sclerotinia sclerotiorum and isolates of 15 other fungal species commonly found associated with onion seed. The primers reliably detected 10 fg of genomic DNA per PCR reaction extracted from pure cultures of B. aclada and B. allii. Conventional assays of surface-disinfested and nondisinfested seed on an agar medium were used to determine the incidence of neck rot Botrytis spp. associated with each of 23 commercial onion seed lots, and the real-time PCR assay was used to determine the quantity of DNA of neck rot Botrytis spp. in each seed lot. A linear relationship could not be found between the incidence of seed infected with the neck rot Botrytis spp. using the conventional agar seed assays and the quantity of DNA of the neck rot Botrytis spp. detected by the real-time PCR assay. However, the real-time PCR assay appeared to be more sensitive than the conventional agar assay, allowing detection of neck rot Botrytis spp. in 5 of the 23 seed lots that tested negative using the conventional agar seed assay.

show abstract

“…Generally B. allii is considered to be the more prevalent species in many countries (Walker, 1925;Goidanich, 1964;Tichelaar, 1967;Presly, 1985). The fungus is mainly seed transmitted, although, to a lesser extent, sclerotia, infected debris and cull piles are also responsible for initiation of primary infection (Walker, 1925(Walker, , 1926Maude & Presly, 1977b).…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature of production of Botrytis allii conidia on their pathogenicity to harvested white onion bulbs

Bertolini

Tian

1997

Plant Pathology

View full text Add to dashboard Cite

Botrytis allii colonies incubated at low temperatures have been reported to produce larger conidia that germinate faster and give rise to longer germ-tubes than those grown at room temperature. The present study compared the effect of conidia produced at 20ЊC and at 0 and -2ЊC on their pathogenicity to artificially inoculated white onion bulbs, and the effect of conidial concentration (5 × 10 3 and 5 × 10 4 conidia/mL) on disease incidence, lesion area, incubation and latent period during storage at 20, 5 and 0ЊC. At all storage temperatures and periods tested conidia produced at -2ЊC caused a higher disease incidence and larger areas of rot than those produced at higher temperatures. When the conidial production temperature was raised to 20ЊC, the duration of incubation on the bulbs inoculated with 5 × 10 4 conidia/mL was more than doubled during storage at 0ЊC, tripled at 5ЊC, and took 50% longer at 20ЊC. The incubation period was not significantly affected by conidial concentration at 20ЊC, and only slightly at 5 and 0ЊC, but at low temperatures the latent period was longer because of the delay induced in sporulation. These data are consistent with the packers' opinion that cross-infection of spring onions by long-term refrigerated onions in grading lines caused earlier and heavier rotting.

show abstract

Studies on Botrytis spp. occurring on onions (Allium cepa) and leeks (Allium porrum)

Cited by 23 publications

References 10 publications

DNA sequence analysis of herbarium specimens facilitates the revival of Botrytis mali, a postharvest pathogen of apple

DNA sequence analysis of herbarium specimens facilitates the revival of Botrytis mali, a postharvest pathogen of apple

A Real-Time, Quantitative PCR Seed Assay for Botrytis spp. that Cause Neck Rot of Onion

Effect of temperature of production of Botrytis allii conidia on their pathogenicity to harvested white onion bulbs

Contact Info

Product

Resources

About