Studies on antioxidant status in Mugil cephalus in response to heavy metal pollution at Ennore estuary

Perumal, Ekambaram; Rani, Munuswamy Usha; Geetha, B.

doi:10.1007/s10661-008-0430-3

Cited by 32 publications

(13 citation statements)

References 16 publications

(11 reference statements)

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“…In our study, the activities of Mn SOD, CAT, GSH-Px and GST in the liver were significantly lower at EB in comparison to reference locality PL. Investigations of other authors also show lower SOD and CAT activities in polluted sites, such as in M. cephalus in India [39] and Dentex labrax in Portugal [40]. Contrary to these findings, some studies demonstrated increased activities of these enzymes in polluted environments [41].…”

Section: Resultsmentioning

confidence: 82%

Antioxidant enzymes in the liver of Chelidonichthys obscurus from the Montenegrin coastline

et al. 2013

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The activities of antioxidant defence enzymes — total, manganese and copper zinc containing superoxide dismutase (Tot SOD, Mn SOD, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and biotransformation phase II enzyme glutathione-S-transferase (GST) — in the liver of longfin gurnard (Chelidonichthys obscurus) from the Montenegrin coastline (Adriatic sea) were investigated. The specimens were collected in winter (February) and late spring (May) at two localities: Platamuni (PL, potentially unpolluted) and the Estuary of the River Bojana (EB, potentially polluted). The obtained results show that the activities of Mn SOD, CAT, GSH-Px and GST in winter were significantly lower at EB than at PL. In spring, the activities of CAT and GST were decreased, while GR activity was increased at EB in comparison to PL. The activities of Mn SOD and GST at PL were decreased and GSH-Px, GR and GST activities at EB were increased in spring compared to winter. Our work represents the first study of liver antioxidant enzymes of longfin gurnard from the Montenegrin coastline and reveals that locality, as a variable, has a greater influence on antioxidant enzymes and biotransformation phase II enzyme GST activities compared to season.

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Section: Resultsmentioning

confidence: 82%

Antioxidant enzymes in the liver of Chelidonichthys obscurus from the Montenegrin coastline

et al. 2013

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“…Due to the rapid industrialization, metal contamination is one of the serious problems that is faced by the Ennore estuary located in North Chennai, Tamilnadu state of India. Heavy metals induce oxidative stress in fish by generating ROS by their participation in Fenton reaction (Padmini et al 2008c). Fish inhabiting the highly polluted Ennore estuary developed an enhanced state of oxidative stress characterized by increased levels of lipid peroxides (LPO), lipid hydroperoxides (LHP), and conjugated diene (CD) when compared to the Kovalam estuary fish in the isolated liver mitochondrial fraction (Padmini and Vijaya Geetha 2007a, b, c).…”

Section: Introductionmentioning

confidence: 99%

“…Pollutants like heavy metals, polycyclic aromatic hydrocarbons (PAH) are found to be the main contaminants of waters of Ennore estuary which contributes to oxidative stress due to their reactive oxygen species generating power (Padmini et al 2008c;Padmini and Vijaya Geetha 2008). Seasonal variation, one of several biological factors have been reported to have a significant influence on estuaries (Wilhelm Filho et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Impact of season on liver mitochondrial oxidative stress and the expression of HSP70 in grey mullets from contaminated estuary

Perumal

Geetha

2008

Ecotoxicology

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Heat shock proteins (HSPs) are the ubiquitous feature of cells in which these proteins cope with stress induced denaturation of other proteins. HSP70 is found to play a primary role in cellular defense under stress condition. In the present investigation, the seasonal impact on environmental stress induced mitochondrial HSP70 (mtHSP70) expression in the liver mitochondria was examined in grey mullets, Mugil cephalus living in the Ennore estuary (polluted site) was compared with the Kovalam estuary (unpolluted site) over the course of two seasons viz monsoon and summer from April 2006 to March 2008. Oxidative stress was determined along with mtHSP70 expression studies in fish liver mitochondria collected from these two estuaries for both the seasons. The liver mitochondria of grey mullet fish collected from polluted Ennore estuary showed increased levels of lipid peroxide and mtHSP70 expression along with decrease in total antioxidant capacity and glutathione redox ratio levels ((c) P < 0.05) when compared to unpolluted Kovalam estuary fish. In the fish liver mitochondria of Ennore estuary, there was significant seasonal variation ((b) P < 0.05) in both oxidative stress marker levels (34% increase) and mitochondrial HSP70 expression (33% increase) with increased level during summer season but the Kovalam estuary fish did not show any significant seasonal variation. In the Ennore estuary fish that are exposed to chronic environmental stress, the overexpression of liver mtHSP70 particularly during summer season may confer differential effects on the cell survival by protection against oxidative stress induced changes.

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“…In the last few decades, attempts were made regarding the evaluation of metal pollution, measurement of metalpollution-induced oxidative stress, and the expression of HSP70 in M. cephalus surviving in this polluted estuary (Padmini et al 2008b;Padmini and Usharani 2008). In Ennore estuary fish liver mitochondrial samples, the comparison of LPO values with the result of the TAC values showed that the TAC level decreases proportionately with increased levels of LPO reflecting the defective antioxidant status when compared with Kovalam estuary samples (Table 1 *p<0.05).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies demonstrated that the fish inhabiting the Ennore estuary are able to survive in the heavy metal pollution induced stressed situation (Padmini et al 2008b). It is important to understand the various damages that occur in these fish liver mitochondria during stress conditions and the mechanism that are involved in providing resistance to such damages which allow the fish to survive.…”

Section: Introductionmentioning

confidence: 99%

Modulation of ASK1 expression during overexpression of Trx and HSP70 in stressed fish liver mitochondria

Perumal

Geetha

2009

Cell Stress and Chaperones

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Mitochondrial heat shock protein 70 (mtHSP70) is found to play a primary role in cellular defense against physiological stress like exposure to environmental contaminants and helpful in the maintenance of cellular homeostasis by promoting the cell survival. In the present investigation, the environmental-stress-induced increase in mtHSP70 levels along with the quantification of apoptosis signal regulating kinase 1 (ASK1) and thioredoxin (Trx) were measured in the liver mitochondria of grey mullets (Mugil cephalus) collected from the polluted Ennore estuary and the unpolluted Kovalam estuary for a period of 2 years. The results showed elevated lipid peroxide (LPO) and decreased total antioxidant capacity along with the decrease in mitochondrial viability percentage. Mitochondrial HSP70, ASK1, and Trx levels were increased under this stress condition. A 42% increase in LPO levels and 18% decrease in mitochondrial survivality were observed in the polluted-site fish liver mitochondria when compared to the results of unpolluted estuary. We also report that, under observed oxidative stress condition in Ennore fish samples, the ASK1 levels are only moderately elevated (13% increase). This may be due to mitochondrial-HSP70-induced adaptive tolerance signaling for the activation of Trx (22% increase) which suppresses the ASK1 expression thereby promoting the cell survival that leads to the maintenance of the cellular homeostasis.

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Studies on antioxidant status in Mugil cephalus in response to heavy metal pollution at Ennore estuary

Cited by 32 publications

References 16 publications

Antioxidant enzymes in the liver of Chelidonichthys obscurus from the Montenegrin coastline

Antioxidant enzymes in the liver of Chelidonichthys obscurus from the Montenegrin coastline

Impact of season on liver mitochondrial oxidative stress and the expression of HSP70 in grey mullets from contaminated estuary

Modulation of ASK1 expression during overexpression of Trx and HSP70 in stressed fish liver mitochondria

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