Studies on Antibody Responses Following Neonatal Immunization with Influenza Hemagglutinin DNA or Protein

Pertmer, Tamera Marie; Robinson, Harriet L.

doi:10.1006/viro.1999.9666

Cited by 44 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA vaccines for mammalian viruses induce strong, long-lasting protective antibody and cytolytic T-cell responses (11,40). The basis for this strong protection is thought to reside in the fact that DNA vaccines produce viral protein within the host cells and this permits presentation of peptides in the context of both major histocompatibility complex class I (MHC-I) and MHC-II molecules.…”

Section: Discussionmentioning

confidence: 99%

DNA Vaccines Encoding Viral Glycoproteins Induce Nonspecific Immunity and Mx Protein Synthesis in Fish

Kim

Johnson

Drennan

et al. 2000

J Virol

162

View full text Add to dashboard Cite

Protective immunity by vaccination with plasmid DNA encoding a viral glycoprotein (G) has long been assumed to result from the induction of a specific immune response. We report here that the initial protection may be due to the induction of alpha/beta interferon, with long-term protection due to a specific response to the encoded viral G. DNA vaccines encoding the Gs of three serologically unrelated fish rhabdoviruses were used to vaccinate rainbow trout against a lethal challenge with infectious hematopoietic necrosis virus (IHNV). All three vaccines, each encoding the G gene of either IHNV (IHNV-G), snakehead rhabdovirus (SHRV) (SHRV-G), or spring viremia of carp virus (SVCV) (SVCV-G), elicited protective immunity against IHNV. Vaccinated fish were challenged at 30 or 70 days postvaccination with lethal doses of IHNV. At 30 days postvaccination, only 5% of fish that had received any of the G vaccines died, whereas more than 50% of the control fish succumbed to virus challenge. When fish were vaccinated and challenged at 70 days postvaccination, only 12% of the IHNV-G-vaccinated fish died compared to 68% for the SHRV-G-and 76% for the SVCV-G-vaccinated fish. Assays for trout Mx protein, an indicator of alpha/beta interferon induction, showed that only fish vaccinated with a G-containing plasmid produced high levels of Mx protein in the kidneys and liver. Interestingly, at day 7 after virus challenge, all of the fish vaccinated with the IHNV-G plasmid were negative for Mx, but the SHRV-G-and SVCV-G-vaccinated fish still showed detectable levels of Mx. These results suggest that DNA vaccines in fish induce an early, nonspecific antiviral protection mediated by an alpha/beta interferon and, later, a specific immune response.Antiviral DNA vaccines carrying a gene for a major antigenic viral protein have received considerable attention as a new approach to vaccine development, especially when traditional vaccines have failed. They offer the advantage of mimicking a viral infection, resulting in host production of a single viral protein that is correctly folded and modified, and eliciting both cellular and humoral immune responses (9, 48). DNA vaccines have been developed for a wide variety of viruses, including influenza virus (14, 46), human immunodeficiency virus (7,15,42), rabies virus (38), hepatitis B virus (10), rubella virus (41), and foot-and-mouth disease virus (19). Genetic vaccines have also been developed for several other pathogens, including Mycoplasma pulmonis (29), Mycobacterium tuberculosis (34), Plasmodium yoelii (17), and Schistosoma japonicum (49).For fish viruses, DNA vaccines have been developed for infectious hematopoietic necrosis virus (IHNV) (2, 33) and viral hemorrhagic septicemia virus (6), both rhabdoviruses belonging to the Novirhabdovirus genus. Laboratory trials with fish indicate that these vaccines are considerably more effective in protecting fish from lethal challenge with homologous virus than either the traditional killed vaccine or the subunit vaccine we had developed previous...

show abstract

Section: Discussionmentioning

confidence: 99%

DNA Vaccines Encoding Viral Glycoproteins Induce Nonspecific Immunity and Mx Protein Synthesis in Fish

Kim

Johnson

Drennan

et al. 2000

J Virol

162

View full text Add to dashboard Cite

show abstract

“…Mice were anesthetized with 0.03 to 0.04 ml of a mixture of 5 ml of ketamine HCl (100 mg/ml) and 1 ml of xylazine (20 mg/ml). Gene gun immunizations were performed on shaved abdominal skin by using the hand-held Bio-Rad gene delivery system as described previously (30,46,47). Mice were immunized with two gene gun doses containing 2 g of DNA per 0.5 mg of approximately 1-m gold beads (Bio-Rad, Hercules, Calif.) at a helium pressure setting of 400 lb/in 2 .…”

Section: Methodsmentioning

confidence: 99%

Enhancement of Antibodies to the Human Immunodeficiency Virus Type 1 Envelope by Using the Molecular Adjuvant C3d

Green

Montefiori

Ross

2003

J Virol

116

112

View full text Add to dashboard Cite

DNA vaccines expressing the envelope (Env) protein of the human immunodeficiency virus have been relatively ineffective at generating high-titer, long-lasting, neutralizing antibodies in a variety of animal models. In this study, the murine and human homologues of the complement component, C3d, were used in a DNA vaccine to enhance the titers of antibody to Env. Initially, plasmids expressing a secreted form of Env (sgp120) fused to one, two, or three copies of the murine homologue of C3d (mC3d) were constructed. Mice were inoculated with four vaccinations of DNA or two DNA vaccinations, followed by two boosts of affinitypurified gp120 protein. Analyses of titers demonstrated that multiple copies of mC3d coupled to sgp120 induced long-lasting, high-titer anti-Env antibody. Priming mice with sgp120-mC3d-DNA, followed by inoculation of purified gp120 protein, elicited the strongest antibody titers; however, the avidity maturation of the antibody was accelerated in the mice inoculated with sgp120-mC3d 3 -DNA. In addition, DNAs expressing sgp120 fused to three copies of the human homologue of C3d (hC3d 3 ) efficiently enhanced the anti-Env antibody in rabbits. Lastly, antisera from both mice and rabbits vaccinated with DNA expressing sgp120-C3d 3 elicited higher titers of neutralizing antibody than did nonfused forms of Env. These results indicate that C3d, conjugated to sgp120, enhances the antibody responses to Env compared to non-C3d fused forms of Env, and this approach may be one way to overcome the poor ability of DNA vaccines to generate antibodies to Env.

show abstract

“…(30). For influenza virus infections, BALB/cJ mice were anesthetized and subsequently infected by intranasal inoculation with 50 l of A/PR/8/34 (H1N1) virus diluted in PBS/2% BSA (0.005-0.01 LD 50 ; 0.00025 hemagglutinating units) (31). Polyoma infections were performed by injecting 2 ϫ 10 6 PFU of virus s.c. into the hind footpads of C3H/HeJ mice (25).…”

Section: Viral and Bacterial Infectionsmentioning

confidence: 99%

CD94/NKG2 Expression Does Not Inhibit Cytotoxic Function of Lymphocytic Choriomeningitis Virus-Specific CD8+ T Cells

Miller

Peters

Oran

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

Murine Ag-specific CD8+ T cells express various NK markers and NK inhibitory receptors that have been proposed to modulate immune responses. Following acute infection of C57BL/6 and BALB/cJ mice with lymphocytic choriomeningitis virus (LCMV), we observed that Ag-specific CD8+ T cells expressed CD94/NKG2. Only slight expression of Ly49A and Ly49C receptors was observed on NP396-specific T cells, while all NP396-specific T cells expressed the NKT cell marker U5A2-13 Ag. Expression of CD94/NKG2 was maintained for at least 1 year following LCMV infection, as was the NKT cell marker. By means of cell sorting and quantitative PCR, we found that NP118-specific CD8+ T cells primarily express transcripts for inhibitory NKG2 receptor isoforms. CD94/NKG2 expression was also observed on Ag-specific CD8+ T cells following infection with polyoma virus, influenza virus, and Listeria monocytogenes, suggesting that it may be a common characteristic of Ag-specific CD8+ T cells following infection with viral or bacterial pathogens. Expression of CD94/NKG2 on memory-specific CD8+ T cells did not change following secondary challenge with LCMV clone 13 and did not inhibit viral clearance. Furthermore, we found no evidence that CD94/NKG2 inhibits either the lytic function of LCMV-specific T cells or their capacity to produce effector cytokines upon peptide stimulation. Finally, down-regulation of CD94/NKG2 was found to occur only during chronic LCMV infection. Altogether, this study suggests that CD94/NKG2 expression is not necessarily correlated with inhibition of T cell function.

show abstract

Studies on Antibody Responses Following Neonatal Immunization with Influenza Hemagglutinin DNA or Protein

Cited by 44 publications

References 28 publications

DNA Vaccines Encoding Viral Glycoproteins Induce Nonspecific Immunity and Mx Protein Synthesis in Fish

DNA Vaccines Encoding Viral Glycoproteins Induce Nonspecific Immunity and Mx Protein Synthesis in Fish

Enhancement of Antibodies to the Human Immunodeficiency Virus Type 1 Envelope by Using the Molecular Adjuvant C3d

CD94/NKG2 Expression Does Not Inhibit Cytotoxic Function of Lymphocytic Choriomeningitis Virus-Specific CD8+ T Cells

Contact Info

Product

Resources

About