Studies on adrenal transplantation. I. Homologous and autogenous transplants

Bodey, Gerald P.; Mioduszewska, O; Gey, George O.

doi:10.1002/aja.1001080204

Cited by 1 publication

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“…None of the expiant cultures was maintained for more than 4 weeks, and only limited attempts to assess steroidogenic activity were made. The expiant culture of adult rat adrenal cortex fragments before transplantation has been performed by Bodey, Mioduszewska & Gey (1961) but with¬ out direct assessment of functional activity. It remains uncertain, therefore, for how long and at what level endogenous steroidogenesis will persist in monolayer cultures of non-proliferating adult adrenocortical cells.…”

Section: Introductionmentioning

confidence: 99%

Morphological Responses to Corticotrophin and Cyclic Amp by Adult Rat Adrenocortical Cells in Monolayer Culture

O'Hare¹,

Am²

1973

Journal of Endocrinology

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Confluent monolayer cultures of cells from the zona fasciculata andzona reticularis of the normal adult rat adrenal cortex were maintained with or without corticotrophin (ACTH) for up to 4 months, without proliferation of adrenal cells. Proliferating fibroblast-like cells, however, eventually overgrew the adrenal monolayer in cultures both with and without ACTH. Adrenocortical cells in culture, maintained without ACTH, spread rapidly to form a confluent monolayer, whereas cell spreading was markedly inhibited in the presence of ACTH. Exposure of previously unstimulated cells to ACTH or cyclic AMP caused the adrenal cells to retract with loss of confluence, the process being reversed when ACTH or cyclic AMP was withdrawn. Ultrastructural features of cells cultured with ACTH were typical of normal adrenocortical cells; in cultures without ACTH they were similar to those of adrenocortical cells found in the hypophysectomized rat.

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Section: Introductionmentioning

confidence: 99%