Studies on adenyl cyclase in Necturus gastric mucosa

Interaction of 3H‐labelled synthetic human gastrin I with rat gastric plasma membranes. Evidence for the existence of biologically reactive gastrin receptor sites

“…This finding is of interest as far as the role of 3',5'-AMP in the regulation of acid secretion is controversial [13]. However, as it will be discussed elsewhere (in preparation), our data are in agreement with the 60% stimulation reported in Necturus gastric mucosa by pentagastrin [2].…”

“…Basal activity in GPM is enriched 2 fold over the homogenate. It is comparable to that previously reported in guinea-pig [13] and rabbit [14] gastric mucosa preparations (22.5 and 12 pmol min -1 mg -1 protein respectively), but higher than that reported in crude membrane fractions prepared from Necturus gastric mucosa [2].…”

“…In addition, the possible mediation of this control by 3',5'-AMP has been suggested [2]. However, no direct evidence has been yet provided for the existence of gastrin receptors in gastric mucosa.…”

Effect of Histamine and Its Methyl Derivatives on Cyclic AMP Metabolism in Gastric Mucosa and Its Blockade by an H2 Receptor Antagonist

“…In both mammalian and nonmammalian species, cyclic AMP appears to act as an intracellular mediator of histamine's action on the oxyntic cells of gastric mucosa (9)(10)(11)(12). A single dose of histamine can stimulate cyclic AMP production in preparations of gastric mucosa…”

“…[334][335][336][337] from guinea pigs and rats (9,11), and a dose-dependent stimulation of adenylate cyclase by histamine has been observed in preparations from mucosa of Nccturus (10). Such relationships have not been found with canine mucosa (13) although gastric juice of dogs contains cyclic AMP and the quantity present appears related to the secretion of H1 (14).…”