Studies on Acetobacter diazotrophicus: Analysis of Nif and Related Genes and Contributions to Sugarcane Nutrition

Sevilla, Myrna; Lee, S.; Brockschneider, D.; Olivera, Antoni; Baldani, Ivo; Kennedy, Christina

doi:10.1007/978-94-011-5159-7_229

Cited by 2 publications

(1 citation statement)

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“…From preliminary results in our laboratory, no nitrogenase expression was detected in planta from an A. diazotrophicus strain containing a nifH^gusA fusion, nevertheless we do not discard the possibility of ben-e¢cial e¡ects of the bacteria in plants grown under other conditions, as have been reported by Sevilla et al [39]. From preliminary results in our laboratory, no nitrogenase expression was detected in planta from an A. diazotrophicus strain containing a nifH^gusA fusion, nevertheless we do not discard the possibility of ben-e¢cial e¡ects of the bacteria in plants grown under other conditions, as have been reported by Sevilla et al [39].…”

Section: Discussionmentioning

confidence: 49%

Colonization of sugarcane by Acetobacter diazotrophicus is inhibited by high N-fertilization

Fuentes-Ramírez

Caballero‐Mellado

Sepúlveda

et al. 1999

FEMS Microbiology Ecology

133

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Acetobacter diazotrophicus is a nitrogen‐fixing endophytic bacterium, originally isolated from sugarcane. Its colonizing ability was evaluated in high and low N‐fertilized sugarcane plants by inoculating stem‐cuts with a β‐glucuronidase marked A. diazotrophicus strain. Bacterial quantification by the most probable number technique showed a severe decrease of A. diazotrophicus cells in plants fertilized with high levels of nitrogen. The inoculated strain was detected inside low N‐fertilized sugarcane plants by histochemical staining of β‐glucuronidase and scanning electron microscopy. A. diazotrophicus was found mainly inside cortical cells of stems and inside xylem vessels. No β‐glucuronidase activity was observed in non‐inoculated plants. High nitrogen fertilization of fields might be a threat to maintaining naturally occurring endophytic associations.

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Section: Discussionmentioning

confidence: 49%