Studies on a new immunoactive peptide, FK-156. II. Fermentation, extraction and chemical and biological characterization.

Abstract

An interesting immunoactive peptide, FK-156, has been isolated from the fermentation broth of Streptomyces olivaceogriseus sp. nov. and Streptomyces violaceus. The compound was purified by column chromatography with activated carbon, ion exchange Sephadex and cellulose powder. FK-156, obtained as white powder, exhibits a wide variety of immunostimulatory activity in vivo and in vitro with experimental animals. Pretreatment of mice or rats with this peptide protected the animals against lethal infection with Escherichia coli and resulted in prolongation of life span of tumor bearing animals. During the course of a screening program directed toward the isolation and evaluation of new immunostimulating agents, we isolated two strains of Streptomyces, strain No. C-353 and strain No.

Effect of anticancer agents neothramycin, aclacinomycin, FK-565 and FK-156 on the release of interleukin-2 and interleukin-1 in vitro

“…FK-156 was isolated from culture filtrates of Streptomyees olivaceogriseus sp. nov. and Streptomyces violaceus [7]. FK-565 is a synthetic analogue of FK-156.…”

Augmentation of Host Resistance against Bacterial Infection by Treatment with Leustroducsin B, a New CSF Inducer

“…In preliminary experiments, daily administration of 0.3 mg/kg LSN B for five days resulted in a sixfold increase in peripheral neutrophils, but no ( FK-156) and isohematinic acid, which enhance the function of neutrophils and thereby augment host resistance to bacterial infection (4,8,13,18). When the activities of some of these compounds are compared with that of LSN B, in our assay system (18), the effects of LSN B were stronger than those of MDP and isohematinic acid, and comparable with those of human recombinant G-CSF (data not shown).…”

Effects of a New Antibiotic, Isohematinic Acid, on the Resistance of Mice to Experimental Infections

“…, and 2-(2-cyanaziridinyl-(1))-2-(2-carbamoyl-aziridinyl-(l))-propane (BM 12,531, Azimexon) have been reported as chemically defined agents (3,8,10). MDP is a minimal requirement for the adjuvant activity of the mycobacterial cell wall in Freund's complete adjuvant and has been shown to be one of the most effective modulators (9).…”

“…MDP is a minimal requirement for the adjuvant activity of the mycobacterial cell wall in Freund's complete adjuvant and has been shown to be one of the most effective modulators (9). FK-156, which was isolated from the culture broth of Streptomyces olivaceogriseus, also stimulates nonspecific resistance to bacterial infections (10). BM 12,531, chemically synthesized as an immunomodulator, has been reported to stimulate immune responses including leucocytosis, phagocytosis, and lymphocyte blast formation (4,6,7).…”