Studies on a membrane-bound nucleotidase from the marine bacterium MB 22. Comparison of a photometric and luminometric method for kinetic investigations

Simonis, Marianne; Rade, Sabine; Ahlers, J.

doi:10.1016/0020-711x(81)90043-4

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Preparation Of Membranes1

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1982

1991

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Cited by 4 publications

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“…Plasma membranes and submitochondrial parti cles from the yeast Saccharomyces cerexisiae (strain R XII, a kind gift from Dr. Kotyk, Prague) were obtained from protoplasts by the procedure described recently [7] , Plasma membranes from the marine bacterium MB 22 were prepared as described by Simonis et al [8] . Membrane-bound and solubilized ATPases from Escherichia coli were obtained by the method reported previously [9].…”

Section: Preparation Of Membranesmentioning

confidence: 99%

Does ^2 H(2)0 Also Protect Membrane-Bound Enzymes?

Ahlers¹,

Foret²,

Lemm³

1983

Enzyme

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Thermal inactivation studies performed with several membrane-bound enzymes such as chitin synthase, cytochrome c oxidase, nucleotidase, as well as bacterial, mitochondrial and plasma membrane ATPases from yeast in H(2)0 and ^2 H(2)0 indicated that most of these enzymes could not be protected by ^2 H(2)0 against thermal inactivation. Only the Escherichia coli ATPase, which is located at the surface of the membrane, and the cytochrome c oxidase were stabilized by heavy water.

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Section: Preparation Of Membranesmentioning

confidence: 99%