Studies on a filarial antigen with collagenase activity

Petralanda, Izaskun; Yarzábal, L. A.; Piessens, Willy F.

doi:10.1016/0166-6851(86)90065-4

Cited by 44 publications

(27 citation statements)

References 19 publications

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“…In Angiostrongylus cantonensis, another nematode that needs to pass through the intestinal wall to initiate its migration through the host, collagenolytic activity associated with metallo-and serine proteases released by the worm has been detected (Lee & Yen 2005). Petralanda et al (1986), working with infective larvae of Brugia malayi, found high collagenase activity in ES products, but only associated with metalloproteases, although collagenolytic activity was also detected in extracts of adults of this species and of another filarian Onchocerca volvulus. These observations led the authors to suggest a possible role of this enzyme in the migration of filarial worms through tissues and also to maintain the integrity of the cuticle.…”

Section: Methodsmentioning

confidence: 99%

“…This prompts the hypothesis that the relative scarcity of nutrients of proteinic origin that could be assimilated by the parasite in the medium without pepsin may lead the worm to search for alternatives through the expression of proteases with collagenolytic activity. This has previously been proposed for filarians (Petralanda et al 1986), and would tend to compensate for the lack of nutrients caused by the absence of pepsin and/or the products generated by it. In fact, in the free-living nematode Caenorhabditis elegans, the presence of metalloproteases with collagenolytic activity and digestive function has recently been detected (Mohrlen et al 2003).…”

Section: Methodsmentioning

confidence: 99%

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Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

Malagón

Adroher

López³

et al. 2010

Dis. Aquat. Org.

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Proteases play a vital role in both the life cycle of parasites and the parasite-host relationship and are considered important virulence factors. In the present study, the presence of proteases with collagenolytic activity was investigated in the fish nematode Hysterothylacium aduncum during in vitro development. Collagenolytic activity was found in all studied developmental stages of the nematode (third [L3] and fourth [L4] larval stages and adults). In L3, the activity was maximum at pH 6.5 and, in the other stages, at 7.0. Pepsin is known to favour in vitro development of the worm, but, in this study, collagenolytic activity was shown to be significantly greater when no pepsin was added to the culture medium (at pH 6.5, p = 0.011). At pH 7.0, most activity was observed in the immature adult, after the final moult, suggesting that the collagenolytic activity may be involved in remodelling of the cuticle and in sexual maturity. On the other hand, at pH 6.5, activity may be related to tissue migration by L3 within the host. Using specific inhibitors, it was demonstrated that most of the collagenolytic activity detected in all the developmental stages was due to metalloproteases (40 to 100%), although serine proteases were also detected in L4 and adults (10 to 30%).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

Malagón

Adroher

López³

et al. 2010

Dis. Aquat. Org.

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“…This differentiation led to suggest that MMPs were secreted by the host not by parasite. Simultaneously, several authors provided information related with the activities of MMPs in different helminthes and MMP mediated histolysis of skin and intestinal walls (Tort et al, 1999) and degradation of ECM proteins (Petralanda et al, 1986). It has been evident that after ingestion, A. cantonensis larvae could invade and penetrate host stomach or intestinal wall .…”

Section: A C T E Dmentioning

confidence: 99%

“…Initial period of MMPs research in nematodes included detection and measurement of activity in different life stages of nematode parasites. Researchers determined collagenilytic activity in extracts of adult worms, in living microfilariae of Onchocerca volvulus and in live infective larvae and adult female worms of Brugi malayi (Petralanda et al, 1986). In culture, infective larvae of B. malayi also secreted large amounts of collagenase.…”

Section: Mmps and Nematode Parasitesmentioning

confidence: 99%

The important role of matrix metalloproteinases in nematode parasites

2014

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SummaryMatrix metalloproteinases (MMPs) represent a large family of over twenty different secreted or membrane-bound endopeptidases, involved in many physiological (embryogenesis, precursor or stem cell mobilization, tissue remodeling during wound healing, etc.), as well as pathological (inflammation, tumor progression and metastasis in cancer, vascular pathology, etc.) conditions. For a long time, MMPs were considered only for the ability to degrade extracellular matrix (ECM) molecules (e.g., collagen, laminin, fibronectin) and to release hidden epitopes from the ECM. However, expressions of many MMPs have been associated with several pathological conditions. It has been established that the MMPs are conserved throughout the animal kingdom and studies of invertebrate have demonstrated that primarily they are involved in various developing functions in hydra, Drosophila, sea urchin and nematodes. The syntheses of these proteolytic enzymes and their release as excretory and secretory products have been reported in various parasitic nematodes. Host invasion and tissue migration of several nematodes have been linked to the expression and release of parasite-derived proteases. Studies with enzyme inhibitors suggest that the enzyme may be a metalloproteinase. Moreover, substrate impregnated zymographic analysis of extracts and excretory and secretory products of different nematode parasites have revealed the multiple enzyme activities of MMPs with various molecular weights. More research on MMP degradome in nematode parasites can provide valuable information for intense evaluation of pathogenesis caused by these parasites.

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“…0. volvulus and B. malayi contain and release proteolytic enzymes that solubilize collagen molecules from native type I collagen fibers (108). Further studies are needed to characterize and determine the role of proteases in the life cycle of the parasite.…”

Section: Vector-parasite Relationships Arthropod Vectorsmentioning

confidence: 99%

Clinical and laboratory aspects of filariasis

Nanduri

Kazura

1989

Clin Microbiol Rev

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Human filarial infections afflict over 150 million persons worldwide and are major causes of morbidity in many developing countries. Onchocerca volvulus infection is a leading preventable cause of blindness, while bancroftian and brugian filariasis may produce lymphatic obstruction of the genitalia and extremities (elephantiasis). Definitive diagnosis of these helminthic infections currently depends on demonstration of microfilariae in host tissues, i.e., the skin in the case of O. volvulus and the bloodstream in the cases of Wuchereria bancrofti and Brugia malayi. Many investigations are now directed at developing specific and sensitive serum antigen assays that will allow diagnosis of active infection (i.e., presence of adult-stage parasites) in the absence of detectable microfilariae. With respect to the immunology of these parasitic infections, efforts are being directed at elucidating the role of T- and B-cell responses in the development of pathologic lesions and resistance to reinfection. These data as well as molecular biologic approaches to identify and study filarial molecules which are immunogenic are discussed. Finally, since treatment of filariases at present depends on antiparasitic drugs, the clinical indications and dosages of diethylcarbamazine and ivermectin are summarized.

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Studies on a filarial antigen with collagenase activity

Cited by 44 publications

References 19 publications

Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

The important role of matrix metalloproteinases in nematode parasites

Clinical and laboratory aspects of filariasis

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