Studies of transepithelial Cl‐ transport in cultured cauda epididymal cells of rats by the short‐circuit current method.

Leung, Ares; Wong, Patrick

doi:10.1113/jphysiol.1992.sp019384

Cited by 19 publications

(11 citation statements)

References 33 publications

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“…The response consisted of an initial spike and a long-term component. Earlier studies have demonstrated that adrenergic stimulation of Isc in the rat epididymal epithelium is largely due to electrogenic Cl À secretion [1,11,15,16]. In the Cl À -free extracellular solution, the initial spike disappeared, and the second component was reduced to about 30% of its prior strength.…”

Section: Discussionmentioning

confidence: 94%

Cellular Mechanisms of Carbachol-Stimulated Cl− Secretion in Rat Epididymal Epithelium1

Ruan

Zuo

et al. 2006

Biology of Reproduction

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Neurotransmitter-controlled Cl- secretions play an important role in maintenance of the epididymal microenvironment for sperm maturation. This study was carried out to investigate the effect of carbachol (CCH) on the cultured rat epididymal epithelium and the signal transduction mechanisms of this response. In normal K-H solution, CCH added basolaterally elicited a biphasic Isc response consisting of a transient spike followed by a second sustained response. Ca2+ activated Cl- channel blocker disulfonic acid stilbene (DIDS, 300 microM) only inhibited part of the CCH-induced Isc response, while nonselective Cl- channel blocker diphenylamine-dicarboxylic acid (DPC, 1 mM) reduced all, indicating the involvement of different conductance pathways. Both peaks of the CCH-induced Isc response could be significantly inhibited by pretreatment with an adenylate cyclase inhibitor, MDL12330A (50 microM). An increase in intracellular cAMP content upon stimulation of CCH was measured. All of the initial peak and part of the second peak could be inhibited by pretreatment with Ca2+-chelating agent BAPTA/AM (50 microM) and an endoplasmic reticulum Ca2+ pump inhibitor, Thapsigagin (Tg, 1 microM). In a whole-cell patch clamp experiment, CCH induced an inward current in the single cell. Two different profiles of currents were found; the first component current exhibited an outward rectifying I-V relationship in a time and voltage-dependent manner, and the current followed showed a linear I-V relationship. The carbachol-induced current was found to be partially blockable by DIDS and could be completely blocked by DPC. The above results indicate that the CCH-induced Cl- secretion could be mediated by Ca2+ and cAMP-dependent regulatory pathways.

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Section: Discussionmentioning

confidence: 94%

Cellular Mechanisms of Carbachol-Stimulated Cl− Secretion in Rat Epididymal Epithelium1

Ruan

Zuo

et al. 2006

Biology of Reproduction

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“…A basolateral Cl/ HCO 3 exchange mechanism has been described in primary cultures of cauda epididymidis [28]. We have recently reported high levels of expression of an Na-HCO 3 cotransporter in the basolateral membrane of epithelial cells lining the initial segment and intermediate zones of the epididymis, and low expression in the more distal parts of the epididymis [29].…”

Section: Introductionmentioning

confidence: 97%

Immunolocalization of AE2 Anion Exchanger in Rat and Mouse Epididymis1

Jensen

Stuart-Tilley

Peters

et al. 1999

Biology of Reproduction

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A low-bicarbonate concentration and an acidic pH in the luminal fluid of the epididymis and vas deferens are important for sperm maturation. These factors help maintain mature sperm in an immotile but viable state during storage in the cauda epididymidis and vas deferens. Two proton extrusion mechanisms, an Na(+)/H(+) exchanger and an H(+)ATPase, have been proposed to be involved in this luminal acidification process. The Na(+)/H(+) exchanger has not yet been localized in situ, but we have reported that H(+)ATPase is expressed on the apical membrane of apical (or narrow) and clear cells of the epididymis. These cells are enriched in carbonic anhydrase II, indicating the involvement of bicarbonate in the acidification process and suggesting that the epididymis is a site of bicarbonate reabsorption. Previous unsuccessful attempts to localize the Cl/HCO(3) anion exchanger AE1 in rat epididymis did not investigate other anion exchanger (AE) isoforms. In this report, we used a recently described SDS antigen unmasking treatment to localize the Cl/HCO(3) exchanger AE2 in rat and mouse epididymis. AE2 is highly expressed in the initial segment, intermediate zone, and caput epididymidis, where it is located on the basolateral membrane of epithelial cells. The cauda epididymidis and vas deferens also contain basolateral AE2, but in lower amounts. The identity of the AE2 protein was further confirmed by the observation that basolateral AE2 expression was unaltered in the epididymis of AE1-knockout mice. Basolateral AE2 may participate in bicarbonate reabsorption and luminal acidification, and/or may be involved in intracellular pH homeostasis of epithelial cells of the male reproductive tract.

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“…Principal cells of the cauda epididymidis and vas deferens secrete bicarbonate following basolateral adrenergic and hormonal stimulation (Carlin et al, 2003;Hagedorn et al, 2007;Leung and Wong, 1992;Pierucci-Alves and Schultz, 2008;Sedlacek et al, 2001;Wong, 1988). This process depends on the presence of CFTR (Wong, 1998), which is located in the apical membrane of principal cells (Pietrement et al, 2008) (Fig.…”

Section: Non-hormonal Regulation Of V-atpase Recycling (Crosstalk Betmentioning

confidence: 99%

Regulation of luminal acidification in the male reproductive tract via cell–cell crosstalk

Shum

Silva

Brown

et al. 2009

Journal of Experimental Biology

120

101

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Studies of transepithelial Cl‐ transport in cultured cauda epididymal cells of rats by the short‐circuit current method.

Abstract: SUMMARY1. Monolayer cultures of cauda epididymides from male Sprague-Dawley rats (210-230 g) were studied by the short-circuit current (Isc)

Cited by 19 publications

References 33 publications

Cellular Mechanisms of Carbachol-Stimulated Cl− Secretion in Rat Epididymal Epithelium1

Cellular Mechanisms of Carbachol-Stimulated Cl− Secretion in Rat Epididymal Epithelium1

Immunolocalization of AE2 Anion Exchanger in Rat and Mouse Epididymis1

Regulation of luminal acidification in the male reproductive tract via cell–cell crosstalk

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