Ali species of Saimiri reported in the literature have 2n = 44 chromosomes. However, a number of morphological variations in karyotypes were described, among them the occurrence of pericentric inversions, correlated by Hershkovifz (1984) with the geographical distributions. Another source of variation was the occurrence of differences in the amount of heterochromatin (Jones and Ma, 1975;Ma and Jones, 1975;Lau et al., 1977).Here we describe the karyotype of S, vanzolinii, described as a new species in the preceding paper, from the general área of the mouth of the Rio Japurá, on the Amazon. Chromosome investigations were performed on skin-derived fibroblasts from one male and one female from the type locality, Lake Mamírauá.G-and C-banding patterns and the distribution of the nucleolar organizer regions were studied (Figs. 1 and 2). The ordering of the chromosomes was based on Jones and Ma (1975). The autosomes were divided in three groups, according to the centromeric position, and were arranged in decreasing order of size in each group (Fig. Ia). Group A has 5 pairs of metacentric chromosomes; pair 2 shows a large secondary constriction in the short arm, corresponding to the nucleolar organizer region (Figs. 1 and 2). Group B has 10 pairs of submetacentric and subtelocentric chromosomes and group C includes 6 pairs of acrocentric chromosomes. The X-chromosome is a submetacentric and the Y is the smallest acrocentric chromosome.In comparing the karyotype of S. vanzolinii with data from the literature it is necessary to note that the paper by Ma (1975) precedes Hershkovitz's (1984) arrangement; we have adapted their data to the latter work. Thus, G-banding revealed a pattern similar to that of S. sciureus macrodon from Leticia, Colombia (Jones and Ma, 1975).After C-banding, heterochromatic blocks were identified around centromeres, on telomeres and interstitially. Whole chromosome arms were also found to be heterochromatic (Fig. 2a). The heteromorphism of chromosome pair 10 in the female karyotype was due to the presence of a large block of heterochromatin at the end of the short arm of only one of the homologues (Fig. 2b).The heterochromatin blocks observed in the long arm of chromosomes 13, 15, 17 and 19 of S. vanzolinii had not been previously described in other Saimiri. On the other hand, the interstitial band in the short arm of chromosome 6 that was described in ali C-banded karyotypes of Saimiri (Ma and