Studies of the Mechanism whereby Adjuvant Disease is Suppressed in Rats Pretreated with Mycobacteria

c-Fos expression in the spinal cord and pain-related symptoms induced by chronic arthritis in the rat are prevented by pretreatment with Freund adjuvant

“…The reduction in inflammatory signs confirm and extend preliminary results on the severity of the disease (Waksman et al, 1960;Gery and Waksman, 1967). We extended these observations and considered the paw diameter and weight, which are indirect indicators of a chronic pain state (Chudler and Dong, 1983;Morton and Griffiths, 1985).…”

“…In addition, the impairment of mobility that is at least partly a consequence of chronic pain states (Larsen and Amt, 1985) was also determined. To explain these effects, it must be remembered that AIA in rats appears to result from an immunological reaction of the cellular type to disseminated constituents of the injected-killed bacteria (see references in Gery and Waksman, 1967). AIA has been shown to be prevented or reduced in rats that had received prior injection(s) of Mycobacterium by any route, since certain antigens present on previously injected bacteria may induce tolerance to arthritogenic epitopes (Gery and Waksman, 1967).…”

“…To explain these effects, it must be remembered that AIA in rats appears to result from an immunological reaction of the cellular type to disseminated constituents of the injected-killed bacteria (see references in Gery and Waksman, 1967). AIA has been shown to be prevented or reduced in rats that had received prior injection(s) of Mycobacterium by any route, since certain antigens present on previously injected bacteria may induce tolerance to arthritogenic epitopes (Gery and Waksman, 1967). The critical Mycobacterium antigen contains an epitope cross-reactive with a self-antigen in joint cartilage, and administration of this cloned epitope antigen in joint cartilage in rats induces resistance to subsequent attempts to produce AIA (van Eden et al, 1988).…”

“…More recently it has been shown that pretreatment with diluted CFA strongly decreases not only the inflammatory pathological signs of the disease, but also the pain-related behaviors (Calvin0 et al,199 1 a). In order to extend the correlation between Fos-LI in lumbar spinal cord neurons and the development of polyarthritis, the aim of the present study was to gauge if a pretreatment with a diluted Mycobacterium butyricum solution, which is known to prevent AIA (Gery and Waksman, 1967), will also prevent C-$X expression.…”

Immune regulation in adjuvant‐induced arthritis: Possible implications for innovative therapeutic strategies in arthritis

“…Lewis rats pretreated with M tuberculosis in saline or in oily adjuvant, either at birth or shortly before immunization, become refractory to the usual sensitizing dose of M tuberculosis in oil while remaining fully susceptible to EAE or bacillus Calmette-Guérin (BCG) sensitization (7,39). Cells obtained from pretreated, sensitized donors are unable to transfer disease, whereas transfer of sensitized cells from untreated donors produces AIA equally readily in M tuberculosis-treated and normal recipients.…”