Studies of the Mechanism of the Periodic Acid-Schiff Histochemical Reaction for Glycogen Using Infrared Spectroscopy and Model Chemical Compounds

Nettleton, G. S.; Carpenter, Anna‐Mary

doi:10.3109/10520297709116750

Cited by 18 publications

(7 citation statements)

References 14 publications

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“…The mechanism underlying the reaction of aldehydes with ordinary Schiff reagent has been recently shown to be an alkylsulphonate-type reaction (Nettleton & Carpenter, 1977). The mechanism underlying the reaction of aldehydes with ordinary Schiff reagent has been recently shown to be an alkylsulphonate-type reaction (Nettleton & Carpenter, 1977).…”

Section: Discussionmentioning

confidence: 99%

A dual staining method for identifying mucins of different gastric epithelial mucous cells

et al. 1991

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A dual staining method has been developed to identify two types of mucous secreting cells in the gastric mucosa of human and rat in one and the same tissue section. Sections were stained first using the galactose oxidase-cold thionin Schiff (GOCTS) procedure and then with paradoxical Concanavalin A staining (PCS). Surface mucous cell mucin stained blue with GOCTS, whereas gland mucous cell mucin stained brown with PCS. This method enabled us to differentiate these two types of mucins not only in gastric epithelial cell cytoplasm but also in the extracellular space. Sugar residues detected by GOCTS were explored by employing four species of lectins, which were peanut and Allomyrina dichotoma agglutinins for beta-galactose and Vicia villosa and Wistaria floribunda agglutinins for beta-N-acetylgalactosamine. The effect of oxidation with galactose oxidase was also examined on the affinities of reactive sites for these lectins. The results indicated that, in the human stomach, the sugar residues responsible for this reactivity were most likely beta-N-acetylgalactosamine and beta-galactose in specimens lacking secretion of blood group determinants and beta-N-acetylgalactosamine in those showing the secretion. In the rat stomach, on the other hand, sugar residues responsible for GOCTS were not elucidated by these lectins.

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Section: Discussionmentioning

confidence: 99%

A dual staining method for identifying mucins of different gastric epithelial mucous cells

et al. 1991

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“…We carried out periodic acid‐Schiff (PAS) staining to detect osteoid deposition for jaw bones and to detect the basal membrane of the epithelium. PAS is a histochemical reaction; the reaction of periodic acid selectively oxidizes the two adjacent hydroxyl residues of glucose molecules to create aldehydes that then react with the Schiff reagent to produce a magenta color (Nettleton & Carpenter 1977). Staining of newly formed osteoid by PAS was carried out following Silbermann et al .…”

Section: Methodsmentioning

confidence: 99%

Bone development in the jaw of Nile tilapia Oreochromis niloticus (Pisces: Cichlidae)

Fujimura

Okada²

2008

Dev Growth Differ

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East African cichlids have evolved feeding apparatus morphologies adapted to their diverse feeding behaviors. The evolution of the oral jaw morphologies is accomplished by the diversity of bone formation during development. To further understand this evolutionary process, we examined the skeletal elements of the jaw and their temporal and sequential emergence, categorized by developmental stages, using the Nile tilapia Oreochromis niloticus as a model cichlid. We found that chondrogenesis started in Stage 17. The deposition of osteoid for the dermal bones commenced in Stage 18. The uptake of calcium dramatically shifted from the surface of larvae to the gills in Stage 20. The bone mineralization of the skeleton began in Stage 25. These data provide important information regarding the sequential events of craniofacial development in East African cichlids and lay the groundwork for studying the molecular mechanisms underlying adaptation of jaw structure to feeding behavior.

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“…The staining intensity was graded semiquantitatively according to the following scale: =no staining; €=weak staining; +=moderate or strong staining. To identify mucus in goblet cells of epithelium and mucinous cells of submucosal gland, the section was stained with the periodic acid-Schiff (PAS) histochemical reaction as described previously (Nettleton and Carpenter 1997;Jember et al 2001). …”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Expression profiles of fibrinolytic components in nasal mucosa

Sejima

Madoiwa

Mimuro

et al. 2004

Histochem Cell Biol

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Components of the fibrinolytic pathway contribute to diverse pathways in many tissues, in addition to their well-recognized role in degradation of fibrin clots. In this study of nasal mucosa, we investigated the presence of mRNA of tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor-1 (PAI-1), and plasminogen activator inhibitor-2 (PAI-2) using reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization, and compared these results with their localization in immunostained tissues. According to real-time RT-PCR results, t-PA, u-PA, PAI-1, and PAI-2 mRNA were noted in human nasal mucosa. Particularly, expression of u-PA and PAI-1 mRNA was significantly high in allergic nasal mucosa in comparison with normal mucosa. t-PA mRNA was detected in endothelial cells and epithelium in normal nasal mucosa. t-PA mRNA was detected in mucous cells of allergic submucosal glands, but not in normal glands. In allergic rhinitis, u-PA and PAI-2 mRNA were detected in mucinous cells and epithelium, and PAI-1 mRNA was detected in serous cells and epithelium. Expression of u-PA and PAI-1 mRNA in normal nasal tissues was decreased in contrast to that in allergic nasal tissues. u-PA staining was observed in mucous cells of allergic submucosal glands and the staining pattern of PAI-2 was similar to that of u-PA. PAI-1 was present in serous cells of submucosal glands from allergy samples, while epithelial cells were almost devoid of stain. In contrast, with allergy, immunohistochemical staining of t-PA was negative in submucosal glands, though positive in endothelial cells and epithelium. However, the expression of t-PA mRNA in allergic nasal mucosa was noted in mucous cells. In fibrin autography of nasal discharge, u-PA was markedly activated in the allergic patient. These results suggest that t-PA synthesized in mucous cells is promptly secreted and modifies watery nasal discharge in allergic rhinitis, and that u-PA activity may help the passage of large amounts of rhinorrhea by also reducing its viscosity. A lot of cellular infiltration (eosinophils in particular) was recognized in allergic nasal mucosa. It is most likely that the modifications in expression of PAs and PAIs are due to the local release of cytokines or growth factors from these inflammatory and immune cells.

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Studies of the Mechanism of the Periodic Acid-Schiff Histochemical Reaction for Glycogen Using Infrared Spectroscopy and Model Chemical Compounds

Cited by 18 publications

References 14 publications

A dual staining method for identifying mucins of different gastric epithelial mucous cells

A dual staining method for identifying mucins of different gastric epithelial mucous cells

Bone development in the jaw of Nile tilapia Oreochromis niloticus (Pisces: Cichlidae)

Expression profiles of fibrinolytic components in nasal mucosa

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