Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate

Benediktsson, Indridi; Spampinato, Claudia P.; Schieder, O.

doi:10.1007/978-94-011-0357-2_6

Cited by 3 publications

(2 citation statements)

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“…A great deal of time can be wasted unless this analysis is focused on regenerable cells, which often comprise a small and inaccessible fraction of the target tissue (12,91). Exhaustive experiments to maximize transient expression are also futile if they involve conditions harmful to regeneration or molecular characteristics of transformed cells (8,26). For example, particle bombardment conditions can now be arranged to give highly reproducible results in transient assays, by delivering a large number of copies of potentially transcribable DNA into the nuclei of target cells.…”

Section: Gene Transfer Strategiesmentioning

confidence: 99%

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PLANT TRANSFORMATION: Problems and Strategies for Practical Application

Birch

1997

Annu. Rev. Plant. Physiol. Plant. Mol. Biol.

505

269

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Plant transformation is now a core research tool in plant biology and a practical tool for cultivar improvement. There are verified methods for stable introduction of novel genes into the nuclear genomes of over 120 diverse plant species. This review examines the criteria to verify plant transformation; the biological and practical requirements for transformation systems; the integration of tissue culture, gene transfer, selection, and transgene expression strategies to achieve transformation in recalcitrant species; and other constraints to plant transformation including regulatory environment, public perceptions, intellectual property, and economics. Because the costs of screening populations showing diverse genetic changes can far exceed the costs of transformation, it is important to distinguish absolute and useful transformation efficiencies. The major technical challenge facing plant transformation biology is the development of methods and constructs to produce a high proportion of plants showing predictable transgene expression without collateral genetic damage. This will require answers to a series of biological and technical questions, some of which are defined.

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