Studies of the &lt;b&gt;&lt;i&gt;Listeria monocytogenes&lt;/i&gt;&lt;/b&gt; Cellobiose Transport Components and Their Impact on Virulence Gene Repression

Cao, Thanh Nguyen; Joyet, Philippe; Aké, Francine Moussan Désirée; Milohanic, Eliane; Deutscher, Josef

doi:10.1159/000500090

Cited by 10 publications

(15 citation statements)

References 58 publications

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“…After ilo knockout, the expression levels of most of the virulence genes were up-regulated but were still higher than those of LI. Our results were not completely consistent with the experimental results of Cao et al [ 36 ]. We observed that the downregulation of CelA and CelB after ilo knockout not only down-regulated the cellobiose-induced repression of virulence genes but also down-regulated glucose-induced repression.…”

Section: Discussioncontrasting

confidence: 99%

“…The two-component system response regulator VirR is the second most important virulence regulator after PrfA [ 35 ]. A study [ 36 ] found that LM mutants lacking CelC1 and CelR , genes related to disaccharide metabolism in the PTS pathway, or lack of CelA genes, exhibited low cellulosic sugar consumption. Deletion of CelC1 or CelR prevented the repression of virulence genes caused by disaccharides, but this was not applicable to glucose and fructose.…”

Section: Discussionmentioning

confidence: 99%

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Hemolysin function of Listeria is related to biofilm formation: transcriptomics analysis

Liang

Tian

et al. 2022

Vet Res

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Listeriolysin O (LLO) is the main virulence protein of Listeria monocytogenes (LM), that helps LM escape lysosomes. We previously found that the cellular immune response elicited by L.ivanovii (LI) is weaker than that elicited by LM. We speculated that this may be related to the function of ivanolysin O (ILO). Here, we constructed hemolysin gene deletion strain, LIΔilo, and a modified strain, LIΔilo::hly, in which ilo was replaced by hly. Prokaryotic transcriptome sequencing was performed on LI, LIΔilo, and LIΔilo::hly. Transcriptome differences between the three strains were compared, and genes and pathways with significant differences between the three strains were analyzed. Prokaryotic transcriptome sequencing results revealed the relationship of ilo to the ribosome, quorum sensing, and phosphotransferase system (PTS) pathways, etc. LIΔilo exhibited attenuated biofilm formation ability compared to LI. Biofilm formation was significantly recovered or even increased after replenishing hly. After knocking out ilo, the relative expression levels of some virulence genes, including sigB, prfA, actA, smcL, and virR, were up-regulated compared to LI. After replenishing hly, these genes were down-regulated compared to LIΔilo. The trend and degree of such variation were not completely consistent when cultured in media containing only monosaccharides or disaccharides. The results confirmed that hemolysin is related to some important biological properties of Listeria, including biofilm formation and virulence gene expression levels. This is the first comprehensive study on ILO function at the transcriptomic level and the first evidence of a relationship between Listeria hemolysin and biofilm formation.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hemolysin function of Listeria is related to biofilm formation: transcriptomics analysis

Liang

Tian

et al. 2022

Vet Res

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“…In L. monocytogenes , cellobiose strongly represses virulence gene expression by inhibiting PrfA, the virulence gene activator [14]. Interestingly another study demonstrated the presence of cellobiose PTS operon to be responsible for increased neuro-invasiveness in hypervirulent L.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an operon required for growth on cellobiose in Clostridioides difficile

2021

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Cellobiose metabolism is linked to the virulence properties in numerous bacterial pathogens. Here, we characterized a putative cellobiose PTS operon of Clostridiodes difficile to investigate the role of cellobiose metabolism in C. difficile pathogenesis. Our gene knockout experiments demonstrated that the putative cellobiose operon enables uptake of cellobiose into C. difficile and allows growth when cellobiose is provided as the sole carbon source in minimal medium. Additionally, using reporter gene fusion assays and DNA pulldown experiments, we show that its transcription is regulated by CelR, a novel transcriptional repressor protein, which directly binds to the upstream region of the cellobiose operon to control its expression. We have also identified cellobiose metabolism to play a significant role in C. difficile physiology as observed by the reduction of sporulation efficiency when cellobiose uptake was compromised in the mutant strain. In corroboration to in vitro study findings, our in vivo hamster challenge experiment showed a significant reduction of pathogenicity by the cellobiose mutant strain in both the primary and the recurrent infection model – substantiating the role of cellobiose metabolism in C. difficile pathogenesis.

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“…Sugar Phosphotransferase System (PTS) mediates the uptake and phosphorylation of carbohydrates and controls the carbon and nitrogen metabolism in response to the availability of sugars [53]. In Listeria monocytogenes, two pairs of soluble PTS components (EIIACel1/ EIIBCel1 and EIIACel2/EIIBCel2) and the permease EIICCel1 were responsible for cellobiose uptake and repression of PrfA, which is a virulence gene activator [54]. As a virulence gene, the ptsP mutant caused a decrease in the colonization ability and pathogenicity of Legionella pneumophila [55].…”

Section: The Downregulated Expression Of Virulencerelated Factors Maymentioning

confidence: 99%

Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis

Ni¹,

Wang

et al. 2020

Virulence

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Streptococcus suis: serotype 2 (S. suis 2) is an important swine pathogen and also an emerging zoonotic agent. HtpsA has been reported as an immunogenic cell surface protein on the bacterium. In the present study, we constructed an isogenic mutant strain of htpsA, namely ΔhtpsA, to study its role in the development and virulence of S. suis 2. Our results showed that the mutant strain lost its typical encapsulated structure with decreased concentrations of sialic acid. Furthermore, the survival rate in whole blood, the anti-phagocytosis by RAW264.7 murine macrophage, and the adherence ability to HEp-2 cells were all significantly affected in the ΔhtpsA. In addition, the deletion of htpsA sharply attenuated the virulence of S. suis 2 in an infection model of mouse. RNA-seq analysis revealed that 126 genes were differentially expressed between the ΔhtpsA and the wild-type strains, including 28 upregulated and 98 downregulated genes. Among the downregulated genes, many were involved in carbohydrate metabolism and synthesis of virulence-associated factors. Taken together, htpsA was demonstrated to play a role in the morphological development and pathogenesis of the highly virulent S. suis 2 05ZYH33 strain.

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Studies of the <b><i>Listeria monocytogenes</i></b> Cellobiose Transport Components and Their Impact on Virulence Gene Repression

Cited by 10 publications

References 58 publications

Hemolysin function of Listeria is related to biofilm formation: transcriptomics analysis

Hemolysin function of Listeria is related to biofilm formation: transcriptomics analysis

Characterization of an operon required for growth on cellobiose in Clostridioides difficile

Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis

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