Studies of the Heterogeneity of a<i>Candida cylindracea</i>(<i>rugosa</i>) Lipase: Monitoring of Esterolytic Activity and Enantioselectivity by Chiral Liquid Chromatography

Allenmark, Stig; Ohlsson, Ann

doi:10.3109/10242429209014897

Cited by 30 publications

(2 citation statements)

References 16 publications

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“…These isoenzymes and/or isoforms have different activities and stereospecificities for many fine chemical synthesis reactions 7. 13, 14 The enantiomeric ratio ( E ) of pure CRL isoenzymes in the hydrolysis of Naproxen methyl ester was 186 for CRL A and 60 for CRLB 15. It has been speculated that enhanced enantioselectivity in repeated batch reactions might be related to mutual transformation of CRL isoenzymes during the reaction.…”

Section: Resultsmentioning

confidence: 99%

Predicting missing markers in human motion capture using l1‐sparse representation

Xiao

Feng

2011

Computer Animation & Virtual

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Missing marker problem is very common in human motion capture. In contrast to most current methods which handle this problem based on trying to learn a reliable predictor from the observations, we consider it from the perspective of sparse representation and propose a novel method which is named l1-sparse representation of missing markers prediction (L1-SRMMP). We assume that the incomplete pose can be represented by a linear combination of a few poses from the training set and the representation is sparse. Therefore, we cast the predicting missing markers as finding a sparse representation of the observable data of the incomplete pose, and then we use it to predict the missing data. In order to get a sparse representation, we employ l1-norm in our objective function. Moreover, we propose presentation coefficient weighted update (PCWU) algorithm to mitigate the limited capacity problem of the training set. Experimental results demonstrate the effectiveness and efficiency of our method to predict the missing markers in human motion capture.

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Section: Resultsmentioning

confidence: 99%

Predicting missing markers in human motion capture using l1‐sparse representation

Xiao

Feng

2011

Computer Animation & Virtual

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“…Many lipases have shown their enantioselectivity for the hydrolysis of esters or synthesis of esters from acids and alcohols (Allenmark and Ohlsson, 1992;Russel and Klibanov, 1988;Weissfloch and Kazlauskas, 1995;Wen et al, 1996). Our previous paper (Kwon, 1992) showed that some lipases had a high enantioselectivity for synthesis of esters.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric Synthesis of (S)-2-Methylbutanoic Acid Methyl Ester, Apple Flavor, Using Lipases in Organic Solvent

Kwon

Hong

Yoon

2000

J. Agric. Food Chem.

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Enantiomeric selective synthesis of (S)-2-methylbutanoic acid methyl ester, which is known as a major apple and strawberry flavor, was performed from racemic 2-methylbutanoic acid using lipases in organic solvent. Among 20 lipases, lipase IM 20 (immobilized lipase of Rhizomucor miehei), lipase AP (Aspergillus niger), and lipase FAP-15 (Aspergillus javanicus) exhibited higher enzymatic activities and enantioselectivities and were selected for the synthesis of (S)-2-methylbutanoic acid methyl ester. Using these enzymes, the reaction conditions such as temperature and lyophilizing pH were optimized, and kinetic parameters were determined. All of the reactions were performed in isooctane, which was identified as the best reaction media for nonaqueous systems. At 20 degrees C maximum enantiomeric excess was observed, while synthetic activity increased as the temperature increased. Only lipases lyophilized at pH 5.5, 6. 0, 6.5, and 7.0 showed synthetic activity. In this pH range, enantioselectivities were not influenced by the lyophilizing pH. The K(M,S) and K(M,R) values for ester synthetic activity of lipase were 1120 and 1240 mM, respectively. Enzyme activity was inhibited by (S)-2-methylbutanoic amide, and its K(i) was calculated as 84 mM. (S)-2-Methylbutanoic amide acted as a competitive inhibitor.

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Analysis of a lipase-catalyzed kinetic resolution by chiral normal-phase liquid chromatography

Löwendahl

Allenmark

1997

Biomed. Chromatogr.

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Studies of the Heterogeneity of aCandida cylindracea(rugosa) Lipase: Monitoring of Esterolytic Activity and Enantioselectivity by Chiral Liquid Chromatography

Cited by 30 publications

References 16 publications

Predicting missing markers in human motion capture using l1‐sparse representation

Predicting missing markers in human motion capture using l1‐sparse representation

Enantiomeric Synthesis of (S)-2-Methylbutanoic Acid Methyl Ester, Apple Flavor, Using Lipases in Organic Solvent

Analysis of a lipase-catalyzed kinetic resolution by chiral normal-phase liquid chromatography

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