Studies of the effect of Ψ-APONIN from Nannochloris sp. on the Florida red tide organism Karenia brevis

Derby, Melissa; Galliano, Michael; Krzanowski, Joseph J.; Martin, Dean F.

doi:10.1016/s0041-0101(02)00285-4

Cited by 6 publications

(9 citation statements)

References 11 publications

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“…Derby et al (2003) reported 95% inhibition by adding partly purified PbTx 3 from Karenia brevis cell extract in a similar bioassay. However, the brevetoxin fraction used could have been contaminated by bioactive compounds other than brevetoxins that have been described for this species (Kubanek et al, 2005;D.F.…”

Section: Discussionmentioning

confidence: 94%

“…The diluent to revive the dormant bacteria was replaced by the seawater based algal medium to have a proper control. The seawater stimulated the bacterial luminescence, a phenomenon reported earlier with the use of seawater in a Microtox analyser (Derby et al, 2003;Onorati & Mecozzi, 2004). To correct for absorbance and scattering of luminescence by the algal cells added to the bacterial suspension the extinction of an algal control had to be measured at 490 nm, the wave length of the emitted light produced by V. fischeri.…”

Section: Discussionmentioning

confidence: 99%

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Evidence for inhibition of bacterial luminescence by allelochemicals from Fibrocapsa japonica (Raphidophyceae), and the role of light and microalgal growth rate

et al. 2007

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The marine microalga Fibrocapsa japonica Toriumi and Takano (Raphidophyceae) produces haemolysins, neurotoxins and reactive oxygen species (ROS). To quantify potential effects of such bioactive compounds on surrounding organisms the marine bacterium Vibrio fischeri was exposed to F. japonica culture samples. Inhibition of V. fischeri 's natural luminescence, indicative of impaired metabolism, was related to the number of F. japonica cells added. The effect was fast, within 15 min. It was caused by one, possibly several, excreted substances that were less active after heating. Freezing of culture supernatant partly inactivated these substances, but ROS-scavenging enzymes had no effect. Light enhanced the V. fischeri luminescence inhibition in two ways. The direct effect of light on the action of F. japonica luminescence inhibiter(s) could be described by a saturation curve with maximum effect above 20 mmol photons m À2 s À1 . Light also had an indirect effect: biomass production, dependent on light availability, was closely related to the amount of inhibiting compound(s) produced by the alga. Algal growth rate, rather than its cell density, determined the bacterial luminescence inhibition per F. japonica cell, resulting in a 5-fold stronger inhibition at maximum growth rates compared to cells that barely grew during the stationary growth phase. The bioassay with F. japonica and V. fischeri has allowed quantification of the negative effects on bacteria in the microalgal microenvironment. The results presented here suggest that at favourable growth conditions F. japonica releases bioactive compounds that improve its competitive abilities.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Evidence for inhibition of bacterial luminescence by allelochemicals from Fibrocapsa japonica (Raphidophyceae), and the role of light and microalgal growth rate

et al. 2007

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“…Steidinger (1983) argued that field application of either the organism Nanochloris or the extracted compound was not practical. Later Martin and Taft (1998) and Derby et al (2003) reported that Aponin does not lyse K. brevis and that it does induce a resting stage. Experimentation to validate the ''resting stage'', which was reported to be a swollen cell that did not lyse and retained its toxins, has not been done.…”

Section: Prevention and Controlmentioning

confidence: 98%

“…(=Gomphosphaeria aponina in part) was also studied as a control agent (Kutt and Martin, 1975;Eng-Wilmont et al, 1977;Hitchcock and Martin, 1977;McCoy and Martin, 1977;Martin, 1980, 1981;Martin and Taft, 1998;Derby et al, 2003). At first it was reported that the organism itself or the extracted active compound would cause irreversible cytolysis to K. brevis (Moon and Martin, 1980).…”

Section: Prevention and Controlmentioning

confidence: 99%

Historical perspective on Karenia brevis red tide research in the Gulf of Mexico

2009

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“…[2] The criticism would appear to be reasonable and to represent what might be termed "conventional wisdom," except for two observations, both of which demonstrated that the expected result was not observed. [9] Secondly, the toxicity of Karenia brevis and Nannochloris sp. that caused Karenia brevis to lose mobility and form spherical species.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nannochloris sp. on the Toxicity of Four Algae

Derby

Krzanowski

Martin³

2005

Journal of Environmental Science and Health, Part A

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Oxidative stress generating active oxygen species has been proved to be one of the underlying agents causing tissue injury after the exposure of Eucalyptus (Eucalyptus spp.) plants to a wide variety of stress conditions. The objective of this study was to perform data mining to identify favorable genes and alleles associated with the enzyme systems superoxide dismutase, catalase, peroxidases, and glutathione S-transferase that are related to tolerance for environmental stresses and damage caused by pests, diseases, herbicides, and by weeds themselves. This was undertaken by using the eucalyptus expressed-sequence database (https//forests.esalq.usp.br). The alignment results between amino acid and nucleotide sequences indicated that the studied enzymes were adequately represented in the ESTs database of the FORESTs project.

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Studies of the effect of Ψ-APONIN from Nannochloris sp. on the Florida red tide organism Karenia brevis

Cited by 6 publications

References 11 publications

Evidence for inhibition of bacterial luminescence by allelochemicals from Fibrocapsa japonica (Raphidophyceae), and the role of light and microalgal growth rate

Evidence for inhibition of bacterial luminescence by allelochemicals from Fibrocapsa japonica (Raphidophyceae), and the role of light and microalgal growth rate

Historical perspective on Karenia brevis red tide research in the Gulf of Mexico

Effect of Nannochloris sp. on the Toxicity of Four Algae

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