Studies of the antigenicity and allergenicity of phospholipase A of bee venom

Light, Wilma C.; Reisman, Robert E.; Ilea, Viorel S.; Wypych, John I.; Okazaki, Tadao; Arbesman, Carl E.

doi:10.1016/0091-6749(76)90138-x

Cited by 16 publications

(6 citation statements)

References 15 publications

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“…It mainly acts as an allergen working with other components for defense of the colony against predators and intruders (King et al, 2000;Sobotka et al, 1976;Light et al, 1976). Recently, a wide variety of pharmacological properties such as neu-…”

Section: Introductionmentioning

confidence: 97%

“…PLA 2 from bee venom is a typical group III secreted PLA 2 , which represents about 12% of the dry weight of venom in A. mellifera (Habermann, 1972). It mainly acts as an allergen working with other components for defense of the colony against predators and intruders (King et al, 2000;Sobotka et al, 1976;Light et al, 1976). Recently, a wide variety of pharmacological properties such as neu-Archives of Insect Biochemistry and Physiology rite outgrowth induction were also reported (Nakashima et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Zhang

Shen

et al. 2005

Arch. Insect Biochem. Physiol.

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Phospholipase A(2) (PLA(2)) is one of the components of bee venom with a wide range of pharmacological functions. It operates as a major allergen working with other venom components to defend the colony from intruder. In the present study, the cDNA sequence of the Ac-pla(2) gene from cDNA library of the venom gland of Apis cerana was compared with the amplified corresponding region of genomic DNA. The result showed that the Ac-pla(2) gene consisted of four exons and three introns. Southern blot showed that the Ac-pla(2) gene was a single copy per haploid genome. The most active transcription period was during the first 8 days of adults, which correspondingly was the period of sharp increase of PLA(2) protein. ELISA analysis revealed that the PLA(2) was undetectable in pupal stage and the newly eclosed adult, but increased sharply to a maximum of 10-12 mug per honeybee by 8-10 days of adult life, followed by a gradual decrease to 8 mug for the rest of adult life. Transcriptional or post transcriptional regulation is the key step for Ac-pla(2) expression. The early secreted Ac-PLA(2) showed a low degree of post-translational modification; with increasing age, glycosylation was detected by Western blot and glycoprotein staining analysis. Different post-translational modifications were found among different individuals in A. cerana when compared to A. mellifera.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Zhang

Shen

et al. 2005

Arch. Insect Biochem. Physiol.

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“…Although conventional venom extracts are used to measure serum-specific IgE in commercially available immunoassay systems, venom is a complex of many substances. For example, the major component allergens of honeybee venom include phospholipase A2 and hyaluronidase,3,4 and those of Vespidae include phospholipase A1, antigen 5, and hyaluronidase 5. Despite the component allergen differences between honeybee and Vespidae venoms, >50% of hymenoptera venom allergy patients react to both venoms in serologic tests 6…”

Section: Introductionmentioning

confidence: 99%

Clinical Features and the Diagnostic Value of Component Allergen-Specific IgE in Hymenoptera Venom Allergy

Shin

Liu

Hur

et al. 2012

Allergy Asthma Immunol Res

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PurposeAlthough patient history is vital for the diagnosis of hymenoptera venom allergy, specific IgE detection is also important to identify the culprit insect and monitor the effect of immunotherapy. We evaluated the diagnostic value of serum-specific IgE detection of hymenoptera venom component allergens and documented changes in allergen-specific IgE after immunotherapy.MethodsFifty-six hymenoptera venom allergy patients receiving venom immunotherapy were recruited from Ajou University Hospital, Korea. The clinical manifestations of the patients were noted, and serum-specific IgE detection was performed, using conventional venom extracts as well as component allergens. Data were analyzed retrospectively.ResultsA total of 35 (62.5%) patients were male, and 33 (73.3%) patients were atopic. The mean patient age was 44.9±13.8 years. Localized reactions occurred in 23.2% of patients, and systemic reactions occurred in 76.8%. The most common clinical manifestations included skin involvement, such as urticaria and angioedema, and respiratory involvement. Yellow jackets were the most frequent culprit insect, followed by yellow hornets, white-faced hornets, honeybees, and paper wasps, as determined at the time of diagnosis. Double sensitization to both Apidae and Vespidae species was detected in 70.9% of patients. The positive predictive values (PPV) of rVes v 5-specific and rPol d 5-specific IgE detection were 85.7% and 87.5%, respectively, which correlated well with conventional venom extract-specific IgE detection (r=0.762 and r=0.757, respectively). In contrast, the PPV of rApi m 1-specific IgE detection at the time of diagnosis was 34.8%. Three years of venom immunotherapy resulted in decreased venom-specific IgE, particularly IgE specific for Vespidae venom components.ConclusionsStings by yellow jackets and male sex may be risk factors for hymenoptera venom allergy in Korea. Vespidae component-specific IgE, but not Apidae component-specific IgE, had diagnostic and monitoring value in hymenoptera venom allergy comparable to that of conventional hymenoptera venom extract-specific IgE.

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“…Bromcyanid-activated paper discs were coupled with 20 [.ig/disc of dandelion and forest honey, respectively, and lOng/disc of rape honey and PG extract as described by Eight et al (13). The optimal amount of antigen coupled was determined by tests with sera from both controls with a negative history of honey allergy and HA patients.…”

Section: Rastmentioning

confidence: 99%

Allergy to honey: relation to pollen and honey bee allergy

Helbling¹,

Peter²,

Berchtold³

et al. 1992

Allergy

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To identify the allergenic components of honey we studied 22 patients with a history of systemic allergic symptoms following honey ingestion. The group of honey-allergic patients was compared with three control groups: 10 subjects sensitized to artemisia, 10 with honey bee venom allergy and 10 without a history of atopy or bee sting reactions. The allergological tests included skin tests and RAST with three different kinds of Swiss honey (dandelion, forest and rape), pollen of compositae species, celery tuber, extract of bee pharyngeal glands, honey bee venom and bee whole body extract. The results show that 3/4 of honey-allergics are sensitive to dandelion honey and 13 of 22 also to compositae pollen. Nine of the honey allergic patients were sensitized to honey bee venom, 3 also to bee pharyngeal glands and to bee whole body extract. Analysis of diagnostic tests and RAST inhibition studies suggest that besides compositae pollen other allergens, most likely of bee origin are important. In honey allergics primary sensitization may be due either to the honey itself, to airborne compositae pollen or even to cross-reacting bee venom components.

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Studies of the antigenicity and allergenicity of phospholipase A of bee venom

Cited by 16 publications

References 15 publications

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

Clinical Features and the Diagnostic Value of Component Allergen-Specific IgE in Hymenoptera Venom Allergy

Allergy to honey: relation to pollen and honey bee allergy

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