Studies of the allergens of Olea europea pollen

Blanca, Miguel; Boulton, P; Brostoff, Jonathan; Gonzalez-Reguera, I.

doi:10.1111/j.1365-2222.1983.tb02624.x

Cited by 47 publications

(27 citation statements)

References 6 publications

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“…Several allergens have been identified in olive pollen (Blanca et al 1983;Vela et al 1982), and two proteins considered major allergens have been purified and characterized (Lauzurica et al 1988b). The major allergen of olive pollen, designated Olea Antigen I (Ole e I), has been separated into two bands of molecular masses 17 and 19 kDa by two-dimensional SDS-PAGE.…”

Section: Introductionmentioning

confidence: 97%

Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

Rodríguez-García

Fernández

Alché

1995

Planta

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Abstract.The monoclonal antibody OL-1 and transmission electron microscopy were used to locate immunologically the major allergen of olive pollen. Ole e I, during pollen grain development. Within the pollen grain, allergenic proteins are located in the cisternae of the rough endoplasmic reticulum. Our findings indicate that the synthesis of these proteins starts in the vegetative cytoplasm of olive pollen during the early maturation stage.

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Section: Introductionmentioning

confidence: 97%

Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

Rodríguez-García

Fernández

Alché

1995

Planta

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“…An ELISA titration with fixed Ole e 3 of this antiserum is shown in ELISA inhibition of the binding to wells coated with Ole e 3 was measured by means of a rabbit antiserum specific for Ole e 3 previously incubated with pollen extracts (500 pg or 50 pg total protein, calculated by the method of Lowry et al [20]) from (1) olive tree, (2) mugwort, (3) birch, (4) ryegrass, ( 5 ) rose, (6) pine, (7) cypress. Mustardseed saline extract (8) was used as control.…”

Section: ---I-p-ev-h--------p--f--f---t-gr--l---------p--vt-mentioning

confidence: 99%

Ole e 3, An Olive‐Tree Allergen, Belongs to a Widespread Family of Pollen Proteins

Batanero

Villalba

Ledesma

et al. 1996

European Journal of Biochemistry

115

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An allergen has been isolated from a saline extract of olive tree (Olea europaea) pollen. The protein consists of a single polypeptide chain of 9.2-kDa, as determined by mass spectrometry. It contains neither tryptophan nor tyrosine residues, and displays an acidic isoelectric point. The secondary structure of the protein, estimated from the analysis of the circular-dichroism spectrum in the peptide-bond region, is composed of 52% a-helix, 10% a-strand, 29% Sturn and 9% non-regular conformation. The N-terminal end of the protein is blocked. Amino-acid-sequence data have been obtained from peptides produced by CNBr treatment of the native allergen. A partial sequence of 36 amino acids has thus been elucidated. The protein exhibits sequence similarity with pollen allergens from Brassica species and contains a Caz'-binding motif. The isolated protein displays IgE-binding activity against sera of patients allergic to olivetree pollen. It has been named Ole e 3, according to the recommendations of the IUIS Nomenclature Committee. IgG ELISA inhibition assays with polyclonal antibodies specific for Ole e 3 reveal the presence of proteins similar to Ole e 3 in the pollen from non-related plant species, which may explain allergic cross-reactivity processes.Keywords: allergen ; olive pollen ; Olea europaea ; Ole e 3 ; protein characterization.Type-I allergy is a clinical disorder that affects more than 15 % of the human population in developed countries. Olive pollinosis is one of the most important causes of type-I allergy in Mediterranean countries, where the olive tree is widely cultivated [l]. Ole e 1 has been shown to be a major allergen from olive pollen, since the IgE of more than 70% of olive-allergic patients recognize this protein [2-41. In addition to Ole e 1, other allergenic proteins have been detected in saline extracts of the olive pollen 15-71, some of which exhibit low molecular masses. Preliminary studies on these molecules were performed with pools of sera from patients hypersensitive to olive pollen. Lauzurica et al. 121 showed the existence of an IgE-reacting protein of 8 kDa upon SDS/PAGE of the pollen extract. Proteins of 7, 9, 14, 15 and 16 kDa, present in olive pollen, have been immunostained with individual sera from olive allergic patients [8]. However, isolation or molecular characterization was not performed for these allergens.Allergic cross-reactivities have been described among Oleaceae members. such as olive, ash and privet [9-131 as well as between olive-pollen and grass-pollen proteins [8]. In addition to profilin, which is an important and well defined panallergen involved in cross-reactions [ 141, other proteins contained in pollen from different species that have conserved sequences could be responsible for allergic processes in individuals not previously sensitized to a given biological source.In spite of the knowledge of a high number of allergenic structures, little is known about their B-cell and T-cell epitopes and the mechanism of induction of allergy. The protein allergens fre...

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“…To date, at least 20 proteins with allergenic activity have been described in olive pollen [4]. Among them, Ole e 1 is the most frequent sensitizing allergen [5, 6].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Polymorphisms in Olive Pollen Allergy: <i>IL13, IL4RA, IL5 </i>and <i>ADRB2</i> Genes

Llanes

Quiralte

López

et al. 2008

Int Arch Allergy Immunol

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Background: Previous results demonstrated that sensitization to specific olive pollen allergens could be related with a different clinical pattern (asthma and/or rhinitis), and that specific patterns of sensitization are regulated by different HLA class II antigens. The authors analyze the possible implication of 7 genetic polymorphisms described as asthma susceptibility genes: IL13 (C–1112T and R130Q), IL4RA (I50V, Q551R), IL5 (C–746T) and ADRB2 (Q27E and R16G) in specific olive pollen allergic sensitization. Methods: The authors genotyped seven polymorphisms of the IL13, IL4RA, IL5 and ADRB2 genes in 146 patients allergic to olive pollen with seasonal rhinitis/asthma and 50 controls using the polymerase chain reaction-restriction fragment length polymorphism and real-time polymerase chain reaction techniques. Results: Two polymorphisms of IL13 were associated with allergy to olive pollen: the TT genotype of IL13 C–1112T was decreased (odds ratio, OR = 0.35, p = 0.006) whereas the RQ heterozygous genotype of IL13 R130Q increased in patients allergic to olive pollen (OR = 3.12, p = 0.009). The combined analysis of two IL4RA single nucleotide polymorphisms (SNPs) (I50V and Q551R) showed an association with asthma: IL4RA V50/Q551 was associated with risk (OR = 2.48, p = 0.007) whereas the IL4RA V50R551 haplotype was associated with protection (OR = 0.31, p = 0.003). Conclusions: The IL13 polymorphisms under study were associated with specific allergy to olive pollen: the IL13 C–1112T polymorphism as a protective factor and the IL13 R130Q polymorphism as a risk factor. Interestingly, although single polymorphisms of IL4RA are not associated with any phenotype analyzed, the interaction between IL4RA I50V/Q551R was strongly associated with the asthma phenotype. IL13 and IL4RA could be relevant markers for allergy to olive pollen and asthma development.

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Studies of the allergens of Olea europea pollen

Cited by 47 publications

References 6 publications

Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

Immunocytochemical localization of allergenic protein (Ole e I) in the endoplasmic reticulum of the developing pollen grain of olive (Olea europaea L.)

Ole e 3, An Olive‐Tree Allergen, Belongs to a Widespread Family of Pollen Proteins

Analysis of Polymorphisms in Olive Pollen Allergy: <i>IL13, IL4RA, IL5 </i>and <i>ADRB2</i> Genes

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