Studies of the Active Substances in Herbs Used for Hair Treatment. II. Isolation of Hair Regrowth Substances, Acetosyringone and Polyporusterone A and B, from Polyporus umbellatus Fries.

Ishida, Hideyuki; Inaoka, Yasunori; Shibatani, Jun-ichi; Fukushima, Makoto; Tsuji, Kiichiro

doi:10.1248/bpb.22.1189

Cited by 22 publications

(9 citation statements)

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“…First, acetosyringone is a potent inducer of the virulence genes of Agrobacterium tumefaciens, and is believed to be the wound signal that attracts Agrobacterium to its host and then facilitates T-DNA transfer (Stachel et al 1985;Sheikholeslam and Weeks 1987). In higher plants, wound-induced acetophenones such as acetosyringone may be limited to members of the Solanaceae (Spencer and Towers 1991), although the compound has been detected as a constitutive phenolic component at relatively high levels in other species, including the herb Polyporus umbellatus (Ishida et al 1999) and the seagrass Posidonia oceanica (Agostini et al 1998). C4H is a wound-induced enzyme (Benveniste et al 1977), and it is therefore tempting to speculate that transgenic over-expression of C4H mimics the metabolic phenotype of wound-induced cells, resulting in acetosyringone production.…”

Section: Discussionmentioning

confidence: 99%

Over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell-suspension cultures

Blount

Masoud

Sumner

et al. 2002

Planta

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Cell-suspension cultures were produced from transgenic tobacco (Nicotiana tabacum L.) plants harboring a constitutively expressed alfalfa cinnamate 4-hydroxylase (C4H) transgene. Increased levels of C4H enzyme activity in the transgenic cultures were observed only following exposure of the cells to yeast elicitor, although alfalfa C4H transcripts were expressed at a high level from the cauliflower mosaic virus 35S promoter in the absence of elicitation. Increased expression of C4H in elicited cell-suspension cultures had no appreciable effect on the HPLC profiles of soluble phenolic compounds. However, levels of one compound, subsequently identified as 3,5-dimethoxy-4-hydroxy acetophenone (acetosyringone), were strongly elevated in the wall-bound phenolic fraction. The results are discussed in relation to the correlation between C4H activity and the synthesis of 3,5-dimethylated hydroxycinnamic acid derivatives in tobacco.

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Section: Discussionmentioning

confidence: 99%

Over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell-suspension cultures

Blount

Masoud

Sumner

et al. 2002

Planta

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“…16,17) Direct protection of the RBC membrane from free radical attack as observed in the present study would provide an important pathophysiological basis for putting to use the helpful hemorrheological, anti-atherosclerotic and anti-inflammatory effects of polyporusterone A, polyporusterone B and Chuling. …”

Section: Discussionmentioning

confidence: 62%

Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

Sekiya

Hikiami

Nakai

et al. 2005

Biological & Pharmaceutical Bulletin

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Since anti-oxidative activitiy may play an important role in the mechanisms of atherogenesis, inflammation and aging, we have searched for new anti-oxidative substances among natural products. We have previously reported that Hoelen (Poria cocos WOLF) was found to have remarkable inhibitory effect on hemolysis induced by 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH), an azo free-radical initiator. 1)Polyporus umbellatus FRIES is a saprophytic fungus growing on withered beech and maple trees roots. Its sclerotium, properly called Chuling, is a crude drug commonly used because of its diuretic effect, like Hoelen. Moreover, Chuling belongs to Basidiomycetes in Eumycetes, also like Hoelen.In this study, we examined the effect of Chuling on the membrane oxidative process. For that purpose, water extract powder was assayed and fractionated in order to isolate the principles responsible for the activity. The effects of Chuling on red blood cells (RBC) hemolysis induced by an azo freeradical initiator in healthy volunteers, and on scavenging reactive oxygen species (ROS) was examined by means of electron spin resonance (ESR) in the present study.Furthermore, we hypothesized that ingestion of Chuling would strengthen the antioxidative effect of plasma if the responsible compounds in Chuling could be absorbed and circulated in blood. Therefore, the present study was to examine the effect on plasma antioxidative activity after a gavagedose of Chuling in rats. MATERIALS AND METHODS Extraction and Fractionation of ChulingDried, ground sclerotia (1.0 kg) of Polyporus umbellatus FRIES (Chuling) were commercially purchased in China and extracted with water (8 l) under reflux for 2 h. The extract was filtered using a stainless steel filter (pore size 150 mm) and lyophilized to afford an aqueous extract (18.5 g). An aliquot of the extract (9.59 g) was first dissolved in water (100 ml), and ethanol was gradually added to a final concentration of 80%. After centrifugation, the precipitate was collected and dried under reduced pressure (P-fraction). The supernatant was fractionated using Diaion HP20 (500 ml, Mitsubishi Chemical Co., Japan), and eluted with 2 l of water (W-fraction), 50% aqueous methanol (50M-fraction), and then methanol (M-fraction), successively. These eluates were concentrated under reduced pressure to yield three fractions. The yields of P-, W-, 50M-, and M-fraction from 9.59 g of aqueous extract were 5.14, 3.40, 0.68, and 0.36 g, respectively. The aqueous extract and their fractions were used as test drugs for the estimation of anti-hemolytic activity.Isolation and Identification of Compounds from Chuling Dried, ground sclerotia (10.0 kg) of Polyporus umbellatus were extracted with methanol (20 l) under reflux for 2 h. The extract was filtered and concentrated to afford a methanolic extract (75 g). The methanolic extract was chromatographed over Diaion HP20 (1.3 l, Mitsubishi Chemical, Tokyo), and eluted with 6 l of water, 50% aqueous methanol, and then methanol, successively. The methanolic fraction (6.65...

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“…The pileus is whitish to yellowish when fresh and becomes darker when dry, the surface is Wbrillose to smooth, and they can be found growing on woods in the northern rain [2]. This species is characterized by poroid hymenophore with large pores (3)(4)(5) per cm) and soft Xesh. It is collected and harvested jointly with other edible mushrooms by diVerent ethnic groups in the forest.…”

Section: Introductionmentioning

confidence: 99%

“…There are no reports of the cultivation of Polyporus species, apparently they were not studied. P. umbellatus is the only one that was commercially cultivated, but this species is tough and is consumed mainly for medicinal purposes as dry powder in tablets [5,26,29]. It is known that the genus Polyporus grows naturally on dead trunks and diVerent lignocellulosic substrates [23], so it is interesting to evaluate if naturally occurring strains of P. tenuiculus could be cultivated on lignocellulosic wastes.…”

Section: Introductionmentioning

confidence: 99%

Polyporus tenuiculus: a new naturally occurring mushroom that can be industrially cultivated on agricultural waste

Omarini

Lechner

Albertó

2009

J Ind Microbiol Biotechnol

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Polyporus tenuiculus is a naturally occurring species from Central and South America that is consumed by different ethnic groups in the region. To determine the optimal conditions for fruiting body production, two strains were assayed on wheat straw and sawdust with or without supplements. Sixty days of incubation at 25 degrees C were needed to produce a solid block. The highest yield was obtained with strain ICFC 383/00 grown on supplemented willow sawdust. In a second experiment the strain ICFC 383/00 and different supplements were used to improve the biological efficiency (BE) and to determine the quality traits and its biodegradation capacity. The highest yields were obtained on sawdust with 25% of supplements reaching 82.7% of BE. Supplements raised the number of flushes, generally from four to five, contributing to increased yields. The type of substrate had a significant effect on fruiting body diameters of P. tenuiculus, and the largest mushrooms were harvested on supplemented substrate with the highest BE coinciding with the highest dry matter loss in substrates. P. tenuiculus showed a capacity to degrade sawdust, causing a decrease of 67.2-74.5% in cellulose, 80.4-85.7% in hemicellulose, and 60.6-66.2% in lignin content at the end of the cultivation cycle. The decrease in hemicellulose was relatively greater than that of cellulose and lignin on supplemented substrates. This is the first report of the cultivation of P. tenuiculus on lignocellulosic waste, and it is a promising species both for commercial production and for its potential use in the degradation of other biowastes.

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Studies of the Active Substances in Herbs Used for Hair Treatment. II. Isolation of Hair Regrowth Substances, Acetosyringone and Polyporusterone A and B, from Polyporus umbellatus Fries.

Cited by 22 publications

References 1 publication

Over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell-suspension cultures

Over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell-suspension cultures

Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

Polyporus tenuiculus: a new naturally occurring mushroom that can be industrially cultivated on agricultural waste

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