Studies of T Lymphocyte Function and Inhibitory Factors in Minimal Change Nephrotic Syndrome

Abstract

Plasma obtained from patients with MCNS in active stage suppressed the ³H-TdR incorporation of autologous or homologous lymphocytes stimulated with PHA-P and E rosette formation, while plasma from patients in remission did not. The depressed mitogenic response of lymphocytes from patients in the active stage significantly improved when the patient’s plasma was substituted for the plasma from inactive MCNS or healthy persons and fetal calf serum. In the preliminary experiment, it was seen that the inhibitory factors were heat stable at 56°C, unadsorbable to charcoal powder and retained in both the permeable and impermeable fractions of collodion bag.

Studies of Vascular Permeability Factor derived from T Lymphocytes and Inhibitory Effect of Plasma on Its Production in Minimal Change Nephrotic Syndrome

“…As we reported previously [12], low molecular weight substances of plasma fraction from MCNS in relapse also had an inhibitory function, sup pressing both the 'H-TdR incorporation of autologous or homologous lymphocytes stimulated with PHA-P and E-rosette formation of the lymphocytes. Based on the results, this low molecular weight inhibitory factor might impair T lymphocytes and reduce or stop the cell func tion which produces VPF in vivo.…”

Monocyte function in idiopathic nephrotic syndrome in childhood.

“…There is no doubt, however, that immune system is concerned with INS. Recent reports have also mentioned abnormality in serum immunoglobulin level (Giangiacomo et al 1975), T cell dysfunction (Wissermann et al 1977;Nagata et al 1979) , the appearance of plasma inhibitory factors (Tomizawa et al 1979) and detection of circulating immune complex (Abras et al, 1979) in minimal change group. It is quite possible that the function of reticuloendothelial system (RES) plays an important role in INS.…”

The Glomerular Polyanion (GPA) of the Rat Kidney

“…It was suggested that the production of this factor in patients suffering from ne phrotic syndrome might be related with the nephrotic status of these patients although the relationship between increased vascular permeability and increased glomerular permeability for macromolecules was not clear. The find ing of a vasoactive lymphokine in these patients may reflect a T cell function disturbance, as was suggested by other investigators studying the nephrotic syndrome [9][10][11]33], Therefore, it would be important to find a relationship of T cell products affecting the glomerular filter structure leading to increased glomerular permeability in vivo. One of the components of the glomerulus associated with selective permeability properties of the filter barrier for macromolecules is the sialoglycoprotein termed glomeru lar polyanion (GPA) [13], which is present predominantly along the epithelial side of the glomerular basement membrane.…”