Studies of potential radiosensitizing agents. Inhibition of nucleic acid synthesis by Synkavit (2-methyl-1,4-naphthaquinol bis disodium phosphate) in Ehrlich mouse ascites tumour cells

Newly synthesized (3)H-labelled DNA was extracted from baby hamster kidney cells (BHK-21/C13 cells) and was shown to possess single-stranded properties when examined by column chromatography on benzoylated naphthoylated DEAE-cellulose, hydroxyapatite and methylated albumin on kieselguhr, and by its affinity for nitrocellulose filters. Some of the newly synthesized DNA was shown to be of lower molecular weight than the bulk of the DNA when examined by alkaline sucrose-density-gradient centrifugation. The properties observed were not affected by treatment of the DNA with ribonuclease, Pronase or amylase. The effect of the size of the DNA on its observed properties was examined and is discussed. It is concluded that DNA synthesis in BHK-21/C13 cells proceeds according to the discontinuous-mechanism model in at least one of the strands.

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Studies of potential radiosensitizing agents. Inhibition of nucleic acid synthesis by Synkavit (2-methyl-1,4-naphthaquinol bis disodium phosphate) in Ehrlich mouse ascites tumour cells

Cited by 5 publications

References 10 publications

Actions of 2-methyl-1,4-naphthoquinone in Bacillus cereus

Actions of 2-methyl-1,4-naphthoquinone in Bacillus cereus

Synkavit Radios en sitizing agent in prostatic carcinoma

Synthesis of deoxyribonucleic acid in BHK-21/C13 cells

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