Studies of Polyarthritis Caused by Mycoplasma arthritidis in Rats I. Detection of the Persisting Mycoplasma Antigen by the Enzyme Immune Assay (EIA) and Conventional Culture Technique

Kirchhoff, H; Heitmann, Jan; Ammar, Ahmed M.; Hermanns, W.; Schulz, L. Cl.

doi:10.1016/s0174-3031(83)80092-4

Cited by 13 publications

(5 citation statements)

References 11 publications

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“…Media and growth of the mycoplasmas. For cultivation of mycoplasmas and tracheal explant cultures, a modified Friis medium (15) similar to that described elsewhere (19) was used, but using CEFOBID (cefoperazone sodium; Roerig Pharmaceuticals) as the antibiotic (250 p.g/liter). The medium was buffered at pH 7.4 with 13 g of HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) per liter.…”

Section: Methodsmentioning

confidence: 99%

Cytopathic effects of Mycoplasma pulmonis in vivo and in vitro

et al. 1991

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This study was performed to evaluate the cytopathic features resulting from Mycoplasma pulmonis infection of tracheal organ cultures compared to with those seen in in vivo infection and to use this system to determine possible differences in cytopathic effects in two M. pulmonis variants found to cause different diseases in vivo. The attachment of M. pulmonis to respiratory epithelium was similar in vivo and in vitro. Cytopathic effects seen in both systems were also similar in loss of tight junctions between cells and exfoliation of respiratory cells, resulting in exposure of the subepithelial layer. These similarities indicate that the observed tissue damage is initiated by the mycoplasmas rather than by immunologic host responses but does not exclude the possibility that host responses may subsequently contribute to the cytopathological events. Comparison of the effects of the two variants (one known to cause death in vivo) did not reveal differences in vitro. This suggests that host factors (not present in vitro) may account for differences in virulence. Detailed in vitro studies allowed the identification of the time frame corresponding to the in vivo infection and also revealed the limitations of the in vitro system.

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Section: Methodsmentioning

confidence: 99%

Cytopathic effects of Mycoplasma pulmonis in vivo and in vitro

et al. 1991

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“…Mycoplasma arthritidis is a causative agent of spontaneous polyarthritis in rats (2) and induces experimental arthritis in rats, mice, and rabbits (1,11,(20)(21)(22)29). In rats and mice, polyarthritis can be elicited by intravenous inoculation with M. arthritidis.…”

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confidence: 99%

Strain differences in sensitivity of rats to Mycoplasma arthritidis ISR 1 infection are under multiple gene control

et al. 1990

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At least 5 female rats from each of 24 inbred (ACI, AS, BDIX, BH, BN, BS, BUF, DA, LE, LEW, MWF, OM, SPRD-Cu3, W-Krypt, and WKY), RTI congenic [BH.lL(LEW), LEW.lA(AVN), LEW.lC(WIST), LEW.lLV3(BH), LEW.lK(SHR), and LEW.1N(BN)], and F1 hybrid [(LEW x BN)F1, (LEW.1W x LEW.1A)F1, and (LEW x LEW.1W)F1] strains, representing eight independent major histocompatibility complex (MHC) haplotypes (a, b, c, dvl, k, 1, n, and u) and five related RTI haplotypes (avi, lvl, 1v3, uv2, and uv3), were inoculated intravenously with Mycoplasma arthritidis, and the severity of the polyarthritis that developed was determined by estimating arthritis scores and weight reductions. The 24 inbred, congenic, and F1 hybrid rat strains differed considerably in their sensitivity to infection with M. arthritidis and in the severity of the polyarthritis that they developed. Statistical evaluation showed that in the acute phase (days 1 to 42 after infection) as well as in the chronic phase (days 39 to 121 after infection) of the disease, the means of the arthritis scores for the strains form a continuous variation without significant interruptions, with the very sensitive LEW rats, the RTI congenic rats on LEW background, the F1 hybrids with LEW, and the MWF, BS, BH, and DA rats on one end and the resistant WKY, BUF, W-Krypt, LE, and OM rats on the other end. A continuous variation was also observed for the means of the growth rates. There were, however, no significant differences between the sensitive and the resistant rat strains in the antibody titers determined by complement fixation test and enzyme immunoassay. Heritabilities of arthritis scores were calculated for all strains (h2 = 0.39 to 0.62), for the RTI congenic strains (h2 = 0.04 to 0.14), and for several strains with identical MHC genes (h2 = 0.61 to 0.93). The results show that non-MHC genes are probably responsible for the sensitivity of rats to infection with M. arthritidis.Animals. Up to 5 female rats from each of 24 inbred (ACI/

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“…The arthritis produced by M. arthritidis has been studied as an animal model for chronic joint diseases by several investigators (COLE et al, 1971;LABER et al, 1975;KIRCHHOFF et al, 1983). So far M. urthritzdis has not been isolated from animals other than rats and mice.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Mycoplasma arthritidis from the Joint Fluid of Boars

Binder

Aumüller

Likitdecharote

et al. 1990

Journal of Veterinary Medicine, Series B

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Summary During an outbreak of a disease in a swine insemination centre in Bavaria, Fed. Rep. of Germany, characterized by conjunctivitis, severe polyarthritis and infertility mycoplasmas have been isolated from the joint fluids of the three boars investigated. Two of the isolate could be typed as Mycoplasma (M.) arthritidis which causes arthritis in rats, mice and rabbits, the third as M. collis, a probably apathogenic rodent mycoplasma species. One of the two isolated M. arthritidis strains (strain D263) was injected intravenously in rats and mice, which developed mild to severe polyarthritis or even died, depending on the numbers of organisms inoculated. Since the bacteriological and virological investigations of the joints of boars did not yield a causative agent, it is to suppose that M. arthritidis played the substantial role in the production of the disease of the boars. It is very likely that the boars catched the mycoplasmas from rodents infected with the isolated species.

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Studies of Polyarthritis Caused by Mycoplasma arthritidis in Rats I. Detection of the Persisting Mycoplasma Antigen by the Enzyme Immune Assay (EIA) and Conventional Culture Technique

Cited by 13 publications

References 11 publications

Cytopathic effects of Mycoplasma pulmonis in vivo and in vitro

Cytopathic effects of Mycoplasma pulmonis in vivo and in vitro

Strain differences in sensitivity of rats to Mycoplasma arthritidis ISR 1 infection are under multiple gene control

Isolation of Mycoplasma arthritidis from the Joint Fluid of Boars

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