Studies of polyamines transport through liquid membranes with D2EHPA as a carrier

Abstract

The transport of polyamines through the liquid membranes with di-2-ethylhexyl phosphoric acid (D2EHPA) was investigated. The study was performed in three main steps: liquid-liquid extraction (LLE), bulk liquid membrane (BLM) extraction, and supported liquid membrane (SLM) extraction. Equilibrium distribution experiments allowed determining the extraction constants and stoichiometric coefficients for each polyamine. It turned out that one amino group binds two molecules of carrier (one D2EHPA dimer) and the extractability of polyamine rises with the increase in number of function groups in the molecule. The BLM and SLM experiments showed that despite considerable differences in distribution ratio between various polyamines the extraction efficiencies for all of them are very approximate. The smaller diamines compensate the lowest affinity to membrane phase with faster interface reaction kinetics and higher diffusivity. Finally, the SLM extraction conditions were optimized. The main parameters that influence the transport are the pH of the donor and acceptor phases. The extraction efficiencies obtained for polyamines are high (80-90%) and give hope for an application in bioanalytical chemistry.

Influence of temperature on mass transfer in an incomplete trapping single hollow fibre supported liquid membrane extraction of triazole fungicides

“…The detailed general theory of the SLM extraction process with a stagnant acceptor has been thoroughly described by Jönsson et al [4]. Several parameters govern the extraction in such a system [7][8][9].…”

A useful procedure for detection of polyamines in biological samples as a potential diagnostic tool in cancer diagnosis

“…Preliminary studies have shown that the modification of the conditions worked-out in previous studies [18] enabled to determine the level of these amines in urine and tissue homogenates.…”

Determination of polyamines in urine via electrospun nanofibers–based solid‐phase extraction coupled with GC‐MS and application to gastric cancer patients

“…Notably, Katarzyna and Casero applied the ion-pairing reagent bis-2-ethylhexylphosphate (BEHPA) to bind the polyamines for extraction, and the complex was broken under the acidic condition (0.1 mol/L HCl solution) for back-extraction [29,30]. Inspired by this, we present an analytical method to extract and determine polyamines and their N-acetylated metabolites from human urine in which the polyamines combine with the ion-pairing reagent in the aqueous phase and are extracted by PFSPE using polystyrene nanofibers as the sorbent.…”