Studies of Intestinal Metaplasia in the Gastric Mucosa by Detection of Disaccharidases With “Tes-Tape”2

Kawachi, Takashi; Kogure, Kikuko; Tanaka, Noritake; Tokunaga, Akira; Sügimura, Takashi; Koyama, Yasuo; Kanasugi, Kazuo; Hirota, Teruyuki; Sano, R

doi:10.1093/jnci/53.1.19

Cited by 51 publications

(22 citation statements)

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“…9,10) Intestinal metaplasia was less pronounced in mucosa surrounding undifferentiated carcinomas, while it was prominent in association with differentiated lesions. Therefore the apparent differences of enzyme activities in surrounding mucosae in the two types of cancers (Table I) can be assumed to be mainly due to the variation in intestinal metaplasia.…”

Section: Discussionmentioning

confidence: 99%

“…Sucrase, which is normally present in the small intestinal striated cell border membrane, 7,8) has been found to be strongly expressed in intestinal metaplasias (considered to be precursor lesions for well-differentiated adenocarcinomas), whereas the enzyme could not be detected in normal gastric mucosas. 9,10) There are at least four alkaline phosphatase isozymes (ALPs), i.e., tissue-unspecific (liver/ bone/kidney), intestinal (I-), placental (P-) and germ cell or placental-like (P-like), whose gene structures were recently classified. [11][12][13][14] In 1976, Miki et al 15) initially reported the co-expression of three types of ALPs: tissue unspecific, P-and I-ALPs in 23 cases of gastric carcinomas.…”

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confidence: 99%

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Increased Expression of Sucrase and Intestinal‐type Alkaline Phosphatase in Human Gastric Carcinomas with Progression

Nakamura

Inada

Hirano

et al. 1998

Japanese Journal of Cancer Research

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The activities of sucrase, total alkaline phosphatase (total ALP) and intestinal-type alkaline phosphatase (I-ALP) were assayed in gastric carcinomas and in their surrounding mucosae from 57 patients with advanced cancers, and the localization of sucrase in 203 carcinomas, including 86 early cancers, was examined immunohistochemically using polyclonal anti-sucrase antibody. All three enzymes were active in the 57 carcinomas as well as in their surrounding mucosae, but the levels were fairly low as compared to those in normal jejunum mucosa. A considerable part of the total ALP activity in tumor specimens was assumed to be due to I-ALP itself. Increased sucrase and I-ALP were found with greater depth of invasion by undifferentiated-type carcinomas. The pattern of immunohistochemical localization of sucrase in the 203 carcinomas also clearly indicated increased expression with greater depth of invasion even in differentiated-type carcinomas.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Increased Expression of Sucrase and Intestinal‐type Alkaline Phosphatase in Human Gastric Carcinomas with Progression

Nakamura

Inada

Hirano

et al. 1998

Japanese Journal of Cancer Research

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“…The present widely applied classification of IM, into complete and incomplete types, was first proposed by Matsukura and colleagues [10] and Kawachi and colleagues [42]. Classification based upon mucin secretion patterns as well as morphology has also allowed division into a small-intestine type and a colonic type [43,44].…”

Section: Classification Of Intestinal Metaplasia (Im)mentioning

confidence: 99%

Gastric-and-intestinal mixed-type intestinal metaplasia: aberrant expression of transcription factors and stem cell intestinalization

2006

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“…Absorptive enterocytes are the most important for the intestinal epithelial cells because the main function of the small intestine is to digest food and absorb nutrients. Disaccharidase activities specific to absorptive enterocytes such as sucrase, maltase, trehalase and lactase were reported in human intestinal metaplastic mucosa, using specimens obtained by gastrectomy or endoscopic gastric biopsy (Classen et al, 1968) (Launiala and Siurala, 1968) (Capoferro, 1972) (Kawachi et al, 1974) (Gudmand-Hoyer et al, 1975. Cdx2 has been reported to regulate the expression of various genes specific to absorptive enterocytes in the intestine such as sucrase-isomaltase (Suh and Traber, 1996), lactase-phlorizin hydrolase (Troelsen et al, 1997), calbindin-D9K (Colnot et al, 1998) and carbonic anhydrase I (Drummond Silberg et al, discussed that other factors specific to small intestine might be needed in addition to Cdx2 for the expression of sucrase.…”

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confidence: 99%

Cdx2 specifies the differentiation of morphological as well as functional absorptive enterocytes of the small intestine

Mutoh

Satoh²,

Kita³

et al. 2005

Int. J. Dev. Biol.

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Many transcription factors are involved in the molecular control of intestinal epithelial cell differentiation. We report in this study that the transcription factor Cdx2 functions to define absorptive enterocytes during intestinal epithelial differentiation. Cdx2 is expressed in the villi of the normal small intestine. Intestinal metaplasia, which expresses Cdx2, occurs as a pathological condition in gastric mucosa. We have previously established Cdx2 transgenic mice expressing Cdx2 exclusively in the gastric epithelium. In this study using Cdx2 transgenic mice, we show that Cdx2 plays a key role in the differentiation of intestinal absorptive enterocytes. The gastric mucosa of Cdx2 transgenic mice was morphologically completely changed into intestinal metaplastic mucosa. Absorptive enterocytes had microvilli which were observed by electron microscope. The intestinal metaplastic mucosa of Cdx2 transgenic mice expressed sucrase and peptide transporter PepT1. Disaccharidase and leucine aminopeptidase activities were observed in the intestinal metaplastic mucosa. Glucose and amino acids were absorbed from Cdx2 transgenic mouse stomach with intestinal metaplasia. Finally we generated mice whose intestine was extensively excised. Cdx2 transgenic mice with intestinal metaplasia survived even after extensive intestinal excision. We successfully demonstrated that Cdx2 induced not only morphological but also functional absorptive enterocytes in the intestinal metaplastic mucosa in vivo. Our results suggest that Cdx2 is necessary and sufficient by itself to specify the development of intestinal absorptive enterocytes, whereas other factors which are expressed in the small intestine are not always necessary for the differentiation of functional absorptive enterocytes.

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Studies of Intestinal Metaplasia in the Gastric Mucosa by Detection of Disaccharidases With “Tes-Tape”2

Cited by 51 publications

References 0 publications

Increased Expression of Sucrase and Intestinal‐type Alkaline Phosphatase in Human Gastric Carcinomas with Progression

Increased Expression of Sucrase and Intestinal‐type Alkaline Phosphatase in Human Gastric Carcinomas with Progression

Gastric-and-intestinal mixed-type intestinal metaplasia: aberrant expression of transcription factors and stem cell intestinalization

Cdx2 specifies the differentiation of morphological as well as functional absorptive enterocytes of the small intestine

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