Studies of in vivo distribution of bovine herpesvirus type 4 in the natural host

Erdei, László; Ballagi-Pordány, A.; Bartha, A; Belák, Sándór

doi:10.1128/jcm.34.5.1091-1095.1996

Cited by 67 publications

(63 citation statements)

References 23 publications

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“…The role of BHV-4 in abortion and various reproductive disorders closely following an infection has been reported by many researchers (Goyal & Naeem 1992;Egyed et al 1996;Czaplicki & Thiry 1998;Frazier et al 2001;Kalman et al 2004). In this study, correlation between BHV-4 and infertility was investigated.…”

Section: Discussionmentioning

confidence: 84%

“…There are many investigations on pathogenesis of BHV-1 on the reproductive system (Miller & Van der Maaten 1985, but studies are limited on BHV-4. Organ distribution (Egyed et al 1996) and virus-isolated cases (Naeem et al 1989;Czaplicki & Thiry 1998;Frazier et al 2001;Kalman et al 2004) show that BHV-4 is an effective virus on various reproductive parameters. Considering the latent character of the infection, it is possible to say that chronic problems may always be seen.…”

Section: Discussionmentioning

confidence: 99%

“…After an experimental infection, the virus accumulates in the internal organs, such as the trachea, the spleen, the lung, the kidney, the liver, the tonsils, and the intestines (Egyed et al 1996). Herpesviruses affect Correspondence: Sibel Gür, Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Virology, ANS Campus, 03200, Afyonkarahisar, Turkey.…”

Section: Introductionmentioning

confidence: 99%

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The possible role of bovine herpesvirus type‐4 infection in cow infertility

Gür¹,

Doğan

2010

Animal Science Journal

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Experimental and field studies have proven that the bovine herpesvirus type-4 (BHV-4) infection leads to various reproductive system problems. In this study, the role of BHV-4 infection in repeat breeding was investigated serologically. Eighty-four samples were obtained from repeat-breeding diagnosed cows in two organized dairy herds; an equal number of healthy cows were sampled from the same farms. The rest of the samples (105) were obtained from reproductively normal cows that were breeding in 18 small enterprises as a control group. The seropositivity proportion in repeat-breeding diagnosed cows was found to be significantly higher (69% (58/84)) than other cows (44% (37/84)) on the same farms. The lowest antibody positivity value for BHV-4 was detected as 24.7% (26/105) in the samples from family-type small farms. The odds ratio (OR) value was calculated as 2.834 in repeat-breeding diagnosed and healthy cows on the same farms, while 6.778 was determined in cows with and without reproductive problems on organized farms compared to small farms. As a result, the BHV-4 infection can be considered one of the reasons for repeat breeding besides other reproductive disorders.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The possible role of bovine herpesvirus type‐4 infection in cow infertility

Gür¹,

Doğan

2010

Animal Science Journal

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“…[19][20][21] Like other gammaherpesviruses, BHV-4 produces persistent low-level infections of lymphoid tissues and peripheral blood mononuclear cells. 7,22,23 Enzymatic amplification of BHV-4 nucleic acid by polymerase chain reaction (PCR) provides a specific, sensitive, and rapid means of detecting low-level BHV-4 infections. 22 To further investigate BHV-4 infections in cats, we developed a 2-stage heminested PCR that uses primer sets designed to amplify a conserved region of the BHV-4 homologue of the herpes simplex virus-1 glycoprotein B (gB) gene.…”

mentioning

confidence: 99%

“…7,22,23 Enzymatic amplification of BHV-4 nucleic acid by polymerase chain reaction (PCR) provides a specific, sensitive, and rapid means of detecting low-level BHV-4 infections. 22 To further investigate BHV-4 infections in cats, we developed a 2-stage heminested PCR that uses primer sets designed to amplify a conserved region of the BHV-4 homologue of the herpes simplex virus-1 glycoprotein B (gB) gene. 2 Glycoprotein B is essential for viral infectivity, and gene sequences encoding gB are highly conserved among herpesviruses.…”

mentioning

confidence: 99%

Prevalence of Bovine Herpesvirus-4 Infection in Cats in Central Michigan

Kruger¹,

Venta²,

Swenson³

et al. 2000

J Vet Int Med

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The gammaherpesvirus bovine herpesvirus-4 (BHV-4) has been isolated from a wide variety of animals, including lions and domestic cats. Although BHV-4 antibodies have been detected in normal cats and cats with urinary disorders, the epidemiology and pathogenic role of BHV-4 in cats is unknown. The purpose of this study was to determine the prevalence of BHV-4 antibodies and viral nucleic acid in a population of free-roaming cats. Plasma and peripheral blood leukocyte samples were collected from 52 male and 52 female free-roaming cats impounded at a regional animal control facility in Central Michigan. Plasma concentrations of BHV-4 antibodies were measured with an indirect fluorescent antibody test. Peripheral blood leukocyte DNA was isolated, and a 2-stage polymerase chain reaction with heminested primers delineating a conserved portion of the BHV-4 glycoprotein B gene homologue was used to amplify BHV-4-specific DNA sequences. BHV-4 antibodies were detected in 38 (73%) male and 23 (44%) female cats. Seropositive cats were significantly more likely to be male than female (odds ratio ϭ 3.22; P ϭ .007). Cell-associated viremia was detected in 17 (33%) male and 11 (21%) female cats. Of the 61 seropositive cats, 23 (38%) had a detectable viremia; only 5 (12%) seronegative cats had detectable viremia. Seropositive cats were significantly more likely to be viremic than seronegative cats (OR ϭ 4.30; P ϭ .009). Our results suggest that BHV-4 infection may be more widespread in certain cat populations than previously reported. Furthermore, many cats seropositive for BHV-4 antibodies have a concurrent cell-associated viremia.Key words: Feline, Gammaherpesvirus, Indirect fluorescent antibody test, 2-stage heminested polymerase chain reaction. Bovine herpesvirus-4 (BHV-4) is a ubiquitous herpesvirus found in healthy cattle and cattle with diverse clinical signs.1 Genomic analyses indicate that BHV-4 is a member of the Gammaherpesvirinae subfamily of lymphotropic herpesviruses and is most closely related to members of the gamma-2 herpesvirus subgroup. and domestic cats. 10-13 BHV-4 also replicates in a number of primary cell cultures and established cell lines originating from cattle, sheep, goats, horses, dogs, cats, rabbits, pigs, chickens, turkeys, mink, ferrets, monkeys, and humans. 10,[14][15][16][17] In 1969, BHV-4 (strain FCAHV) was isolated from a litter of normal kittens and from a cat with cystitis and concurrent upper respiratory disease.10 Induced BHV-4 infections in conventionally reared or specific-pathogen-free cats resulted in clinical signs of conjunctivitis, tracheitis, bronchitis, pneumonia, hemorrhagic cystitis, and urethral obstruction.7,11-13 Detection of serum BHV-4 antibodies in 44 of 141 cats evaluated at a veterinary teaching hospital for lower urinary tract disease and in 6 of 26 clinically normal control cats further supported the concept that BHV- Sciences, Room D-208, Veterinary Clinical Center, Michigan State University, East Lansing, MI 48824-1314; e-mail: kruger@cvm.msu.edu. Submitted Jan...

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