Studies of Human 2,4-Dienoyl CoA Reductase Shed New Light on Peroxisomal β-Oxidation of Unsaturated Fatty Acids

Hua, Tian; Wu, Dong; Ding, Wei; Wang, Jiangyun; Shaw, Neil; Liu, Zhijie

doi:10.1074/jbc.m112.385351

Cited by 18 publications

(14 citation statements)

References 36 publications

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“…Furthermore, RFI has been shown to have a high correlation with obesity on carcase composition in the cows of Limousine, Hereford and Charolaise (Herd and Bishop 2000) and with back fat in pigs (Hoque et al 2007). In this line, the role of AGMO in regulating fat concentration in human and other animals by acting on the nervous system and cytokine in signal transduction has been reported (Hua et al 2012). This gene is the only recognised enzyme, which breaks the alkyl-glycerol ether bond (Taguchi and Armarego 1998).…”

Section: Discussionmentioning

confidence: 88%

Gene expression profile analysis of residual feed intake for Isfahan native chickens using RNA-SEQ data

Izadnia

Tahmoorespur

Bakhtiarizadeh

et al. 2018

Italian Journal of Animal Science

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The factors that affect residual feed intake (RFI) of two chicken breeds (Ross as commercial and Isfahan as Iranian local breed with low and high RFI, respectively) were examined. We sequenced their liver transcriptomes using RNA-Seq while focusing on the identification of important candidate genes, which might influence RFI and growth rate. The differential gene expression analysis revealed that 121 and 279 known genes were significantly up-and downregulated in the local breed, respectively. QTL enrichment analysis revealed that 63 down-regulated genes in the local breed were enriched in the QTL regions related to feed efficiency traits. Moreover, the functional enrichment analysis showed that the down-regulated genes in the native chickens were mainly involved in the different metabolic processes, such as carboxylic acid metabolic process and response to stress. The Differentially Expressed Genes explored between the chickens of the two breeds led to the identification of some important candidate genes for further breed improvement programmes, including RSAD2, IL15, LIPI, EGR1 and DUSP16. These findings will be a useful resource for the biological investigations of RFI-related genes in Isfahan local chickens and may provide some clues for understanding the molecular genetic mechanisms in other chicken breeds. HIGHLIGHTS A global view of transcriptome differences in the liver tissues of commercial and local chicken breeds with different RFI values is obtained. This investigation will contribute to the improvement of chicken genome annotation through the identification of novel transcripts and novel gene. Some important candidate genes related to RFI is obtained.

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Section: Discussionmentioning

confidence: 88%

Gene expression profile analysis of residual feed intake for Isfahan native chickens using RNA-SEQ data

Izadnia

Tahmoorespur

Bakhtiarizadeh

et al. 2018

Italian Journal of Animal Science

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“…For instance, glyoxalase 1 ( GLO1 ) plays a critical part in the enzymatic defense against dysfunctional glycated forms of proteins [45] , the vacuolar protein sorting 52 homolog ( VPS52 ) is involved in the transport and sorting of proteins from the plasma membrane to the lysosome via mannose-6-phosphate receptors [46] , the N-acetylglucosamine-1-phosphate transferase gamma subunit ( GNPTG , top 10 key driver, between GLO1 and GFER in Figure 2 ) is part of mannose-6-phosphate synthesis [47] , and peptidylprolyl isomerase ( PPIL1 ) is a member in the cyclophilin family that regulates protein folding and immune responses [48] . Of note, there may also be a direct link to lipid metabolism: the peroxisomal 2,4-dienoyl CoA reductase ( DECR2 ), which participates in the beta-oxidation of unsaturated fatty acids [49] , is a key driver of this ‘Antigen’ superset and a member of ‘Lipid I’.…”

Section: Discussionmentioning

confidence: 99%

Integrative Genomics Reveals Novel Molecular Pathways and Gene Networks for Coronary Artery Disease

Mäkinen¹,

et al. 2014

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The majority of the heritability of coronary artery disease (CAD) remains unexplained, despite recent successes of genome-wide association studies (GWAS) in identifying novel susceptibility loci. Integrating functional genomic data from a variety of sources with a large-scale meta-analysis of CAD GWAS may facilitate the identification of novel biological processes and genes involved in CAD, as well as clarify the causal relationships of established processes. Towards this end, we integrated 14 GWAS from the CARDIoGRAM Consortium and two additional GWAS from the Ottawa Heart Institute (25,491 cases and 66,819 controls) with 1) genetics of gene expression studies of CAD-relevant tissues in humans, 2) metabolic and signaling pathways from public databases, and 3) data-driven, tissue-specific gene networks from a multitude of human and mouse experiments. We not only detected CAD-associated gene networks of lipid metabolism, coagulation, immunity, and additional networks with no clear functional annotation, but also revealed key driver genes for each CAD network based on the topology of the gene regulatory networks. In particular, we found a gene network involved in antigen processing to be strongly associated with CAD. The key driver genes of this network included glyoxalase I (GLO1) and peptidylprolyl isomerase I (PPIL1), which we verified as regulatory by siRNA experiments in human aortic endothelial cells. Our results suggest genetic influences on a diverse set of both known and novel biological processes that contribute to CAD risk. The key driver genes for these networks highlight potential novel targets for further mechanistic studies and therapeutic interventions.

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“…E). The gene products encoded by these transcripts are present in mitochondria, and are involved in maintaining the potential difference across the inner mitochondrial membrane and maintaining normal physiological function .…”

Section: Resultsmentioning

confidence: 99%

Multipronged approach to identify and validate a novel upstream regulator of Sncg in mouse retinal ganglion cells

et al. 2016

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Loss of retinal ganglion cells (RGCs) is one of the hallmarks of retinal neurodegenerative diseases, glaucoma being one of the most common. Mechanistic studies on RGCs are hindered by the lack of sufficient primary cells and consensus regarding their signature markers. Recently, c-synuclein (SNCG) has been shown to be highly expressed in the somas and axons of RGCs. In various mouse models of glaucoma, downregulation of Sncg gene expression correlates with RGC loss. To investigate the role of Sncg in RGCs, we used a novel systems genetics approach to identify a gene that modulates Sncg expression, followed by confirmatory studies in both healthy and diseased retinae. We found that chromosome 1 harbors an expression quantitative trait locus that modulates Sncg expression in the mouse retina, and identified the prefoldin-2 (PFDN2) gene as the candidate upstream modulator of Sncg expression. Our immunohistochemical analyses revealed similar expression patterns in both mouse and human healthy retinae, with PFDN2 colocalizing with SNCG in RGCs and their axons. In contrast, in retinae from glaucoma subjects, SNCG levels were significantly reduced, although PFDN2 levels were maintained. Using a novel flow cytometry-based RGC isolation method, we obtained viable populations of murine RGCs. Knocking down Pfdn2 expression in primary murine RGCs significantly reduced Sncg expression, confirming that Pfdn2 regulates Sncg expression in murine RGCs. Gene Ontology analysis indicated shared mitochondrial function associated with Sncg and Pfdn2. These data solidify the relationship between Sncg and Pfdn2 in RGCs, and provide a novel mechanism for maintaining RGC health.

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Studies of Human 2,4-Dienoyl CoA Reductase Shed New Light on Peroxisomal β-Oxidation of Unsaturated Fatty Acids

Cited by 18 publications

References 36 publications

Gene expression profile analysis of residual feed intake for Isfahan native chickens using RNA-SEQ data

Gene expression profile analysis of residual feed intake for Isfahan native chickens using RNA-SEQ data

Integrative Genomics Reveals Novel Molecular Pathways and Gene Networks for Coronary Artery Disease

Multipronged approach to identify and validate a novel upstream regulator of Sncg in mouse retinal ganglion cells

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