Studies of genetically defined chimeras of a European type A virus and a South African Territories type 2 virus reveal growth determinants for foot-and-mouth disease virus

Rensburg, H.G. Van; Henry, Tina M.; Mason, Peter W.

doi:10.1099/vir.0.19509-0

Cited by 42 publications

(46 citation statements)

References 25 publications

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“…The nucleotide sequence of the amplicon was determined using selected oligonucleotides and the ABI PRISM™ BigDye™ Terminator Cycle Sequencing Ready Reaction kit v3.0 (Perkin-Elmer Applied Biosystems), followed by resolution on an ABI PRISM 310 Genetic Analyser (Applied Biosystems). The openUP (July 2007) nucleotide sequence of the capsid-encoding region of NAM/307/98 has been submitted to GenBank under the accession number AY770519.…”

Section: Rna Isolation Cdna Synthesis Pcr Amplification and Nucleotmentioning

confidence: 99%

“…Instituto Biologico Renal Suino-2 cells (IB-RS-2) were maintained in RPMI medium (Sigma) and Chinese hamster ovary cells strain K1 (CHO-K1, ATCC CCL-61) were maintained in Ham's F-12 medium (Invitrogen), both supplemented with 10% FCS. Plaque assays were performed using a tragacanth overlay method (Grubman et al, 1979) and 1% methylene blue staining (Van Rensburg et al, 2004). The origin of SAT1/NAM/307/98 (passage history: primary pig kidney passage 1; IB-RS-2 passage 1) and SAT2/ZIM/7/83 (passage history: bovine passage 1; BHK passage 5; bovine passage 1; BHK passage 3) has been described elsewhere (Bastos et al, 2001 andVan Rensburg et al, 2004).…”

Section: Cell Lines Viruses and Plasmidsmentioning

confidence: 99%

“…Plaque assays were performed using a tragacanth overlay method (Grubman et al, 1979) and 1% methylene blue staining (Van Rensburg et al, 2004). The origin of SAT1/NAM/307/98 (passage history: primary pig kidney passage 1; IB-RS-2 passage 1) and SAT2/ZIM/7/83 (passage history: bovine passage 1; BHK passage 5; bovine passage 1; BHK passage 3) has been described elsewhere (Bastos et al, 2001 andVan Rensburg et al, 2004). pSAT2, a previously constructed genome-length infectious cDNA clone of ZIM/7/83 (Van Rensburg et al, 2004), was used as backbone in the construction of chimeric cDNA clones.…”

Section: Cell Lines Viruses and Plasmidsmentioning

confidence: 99%

“…The seven serotypes of FMDV (types O, A, C, Asia1, and the South African Territories [SAT] types 1, 2, and 3) are openUP (July 2007) members of the Aphthovirus genus of the family Picornaviridae (Grubman and Baxt, 2004). The FMDV virion consists of an icosahedral shell composed of 60 copies each of four structural proteins, 1A to 1D, surrounding a single-stranded, positive-sense RNA.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the novel occurrence of two RGD motifs in capsid protein 1D of NAM/307/98, the role of these motifs in receptor binding and cell infection was determined by introducing mutations within the conserved βG-βH loop RGD sequence via a genome-length infectious chimeric cDNA clone. Following in vitro transcription openUP (July 2007) and transfection of BHK cells with the mutated RNAs, the genetically engineered viruses were examined for their infectious potential.…”

Section: Introductionmentioning

confidence: 99%

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A second RGD motif in the 1D capsid protein of a SAT1 type foot-and-mouth disease virus field isolate is not essential for attachment to target cells

et al. 2007

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Section: Rna Isolation Cdna Synthesis Pcr Amplification and Nucleotmentioning

confidence: 99%

Section: Cell Lines Viruses and Plasmidsmentioning

confidence: 99%

Section: Cell Lines Viruses and Plasmidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A second RGD motif in the 1D capsid protein of a SAT1 type foot-and-mouth disease virus field isolate is not essential for attachment to target cells

et al. 2007

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Recombination of host cell mRNA with the Asia 1 foot-and-mouth disease virus genome in cell suspension culture

et al. 2018

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Foot-and-mouth disease virus (FMDV) exhibits high mutation rates during replication. In this study, an isolate of FMDV serotype Asia-1 was serially passaged in a BHK-21 cell monolayer and then adapted to serum-free BHK-21 cell suspension culture to produce a seed virus for production of an inactivated vaccine. Analysis of the sequence encoding the structural proteins of the virus at various passages showed the presence of overlapping peaks in sequencing electropherograms after nucleotide 619 of VP1 in viruses recovered from the fourth passage in suspension culture, suggesting the possible introduction of an insertion or deletion into this portion of the viral genome of our seed virus stock. To evaluate this phenomenon, a virus designated "Vac-Asia1-VDLV", was isolated by plaque purification from the tenth passage in suspension culture. Sequencing results showed that a 12-nt-long exogenous sequence was inserted into the 3' end of the VP1 coding region at the position where the original overlapping peaks were identified. Analysis of the host cell transcriptome showed that the 12-nt sequence was identical to a highly expressed sequence in BHK-21 cells, strongly suggesting that recombination between the FMDV genome and host cell mRNA produced the recombinant virus. A growth curve showed that the virus with the 12-nt insertion reached a peak earlier than the parental strain and that this virus had acquired the ability to bind to the cell surface by a mechanism that was not dependent on integrin or the heparan sulfate receptor. This novel pathogen-host cell recombination event is discussed in terms of the mechanism of viral RNA replication and the phenotypic constraints of FMDV biology and evolution.

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Megaprimer-mediated capsid swapping for the construction of custom-engineered chimeric foot-and-mouth disease virus

et al. 2015

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Foot-and-mouth disease (FMD) is a highly contagious, economically important disease of transboundary importance. Regular vaccination with chemically inactivated FMD vaccine is the major means of controlling the disease in endemic countries like India. However, the selection of appropriate candidate vaccine strain and its adaptation in cell culture to yield high titer of virus is a cumbersome process. An attractive approach to circumvent this tedious process is to replace the capsid coding sequence of an infectious full-genome length cDNA clone of a good vaccine strain with those of appropriate field strain, to produce custom-made chimeric FMD virus (FMDV). Nevertheless, the construction of chimeric virus can be difficult if the necessary endonuclease restriction sites are unavailable or unsuitable for swapping of the capsid sequence. Here we described an efficient method based on megaprimer-mediated capsid swapping for the construction of chimeric FMDV cDNA clones. Using FMDV vaccine strain A IND 40/2000 infectious clone (pA(40/2000)) as a donor plasmid, we exchanged the capsid sequence of pA(40/2000) with that of the viruses belonging to serotypes O (n = 5), A (n = 2), and Asia 1 (n = 2), and subsequently generated infectious FMDV from their respective chimeric cDNA clones. The chimeric viruses exhibited comparable infection kinetics, plaque phenotypes, antigenic profiles, and virion stability to the parental viruses. The results from this study suggest that megaprimer-based reverse genetics technology is useful for engineering chimeric vaccine strains for use in the control and prevention of FMD in endemic countries.

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Studies of genetically defined chimeras of a European type A virus and a South African Territories type 2 virus reveal growth determinants for foot-and-mouth disease virus

Cited by 42 publications

References 25 publications

A second RGD motif in the 1D capsid protein of a SAT1 type foot-and-mouth disease virus field isolate is not essential for attachment to target cells

A second RGD motif in the 1D capsid protein of a SAT1 type foot-and-mouth disease virus field isolate is not essential for attachment to target cells

Recombination of host cell mRNA with the Asia 1 foot-and-mouth disease virus genome in cell suspension culture

Megaprimer-mediated capsid swapping for the construction of custom-engineered chimeric foot-and-mouth disease virus

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