Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality

Zhang, Xingqi; Jafari, Nadereh; Barnes, Randall B.; Confino, Edmond; Milad, Magdy P.; Kazer, Ralph R.

doi:10.1016/j.fertnstert.2004.11.030

Cited by 181 publications

(163 citation statements)

References 27 publications

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“…In fact, although it is known that prolonging the embryo culture period allows for a better selection of embryos for transfer leading to increased implantation rates and reduced risk of twin and higher order pregnancies [38], there is concern that a strategy of blastocyst culture may result in higher cycle cancellation rates [39]. Potential CC biomarkers correlated to oocyte quality, embryo competence, or pregnancy outcome have been identified by microarray analysis [14,24,26,40], RT-PCR [41,42], or quantitative RT-PCR [25,43,44]. Although genomics can supply valuable information regarding expected functions, the proteome is the entire complement of proteins expressed by a genome, and therefore, it is more indicative of the phenotype and can provide important information that is complementary to the data obtained from gene expression studies.…”

Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

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Purpose The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Methods Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negativepregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. Results There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the nonblastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. Conclusions CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.

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Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

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“…Other cytokines and neuronal factors were also shown to be differentially expressed within CCs, and therefore suggested as a mediator of the ovulatory process in many mammal species including humans (McKenzie et al 2004, Zhang et al 2005, Feuerstein et al 2007), mice , Richards 2007, rats , and cattle (Assidi et al 2008).…”

Section: Neuronal-like Functions Of Cumulus Cellsmentioning

confidence: 99%

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Assidi¹,

Dieleman²,

Sirard³

2010

Reproduction

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Cumulus cells (CCs) are essential for oocytes to reach full development competency and become fertilized. Many major functional properties of CCs are triggered by gonadotropins and governed by the oocyte. Consequently, cumulus may reflect oocyte quality and is often used for oocyte selection. The most visible function of CCs is their ability for rapid extracellular matrix expansion after the LH surge. Although unexplained, LH induces the final maturation and improves oocyte quality. To study the LH signaling and gene expression cascade patterns close to the germinal vesicle breakdown, bovine CCs collected at 2 h before and 6 h after the LH surge were hybridized to a custom-made microarray to better understand the LH genomic action and find differentially expressed genes associated with the LH-induced oocyte final maturation. Functional genomic analysis of the 141 overexpressed and 161 underexpressed clones was performed according to their molecular functions, gene networks, and cell compartments. Following real-time PCR validation of our gene lists, some interesting pathways associated with the LH genomic action on CCs and their possible roles in oocyte final maturation, ovulation, and fertilization are discussed. A list of early potential markers of oocyte competency in vivo and in vitro is thereafter suggested. These early biomarkers are a preamble to understand the LH molecular pathways that trigger the final oocyte competence acquisition process in bovine.

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“…Previous researches have proved that different gene expressions of CCs could indicate oocyte competence or predict the efficiency of embryo development and pregnancy outcome (McKenzie et al 2004, Zhang et al 2005, Feuerstein et al 2007, Assou et al 2008, van Montfoort et al 2008, Kenigsberg et al 2009, Adriaenssens et al 2010, Assou et al 2010. Nevertheless, among all these previous studies on the gene expression profiles of human CCs, only one study (Ouandaogo et al 2011) has highlighted the distinct gene expression of human CCs isolated from oocytes at the GV, MI, and MII stages, while others have mainly focused on CCs surrounding the oocytes at MII stage.…”

Section: Introductionmentioning

confidence: 99%

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

Huang¹,

Hao²,

Shen³

et al. 2013

Reproduction

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Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of endocrine and intra-ovarian paracrine interactions may change the microenvironment for oocyte development during the folliculogenesis process and reduce the developmental competence of oocytes in PCOS patients who are suffering from anovulatory infertility and pregnancy loss. In this microenvironment, the cross talk between an oocyte and the surrounding cumulus cells (CCs) is critical for achieving oocyte competence. The aim of our study was to investigate the gene expression profiles of CCs obtained from PCOS patients undergoing IVF cycles in terms of oocyte maturation by using human Genome U133 Plus 2.0 microarrays. A total of 59 genes were differentially expressed in two CC groups. Most of these genes were identified to be involved in one or more of the following pathways: receptor interactions, calcium signaling, metabolism and biosynthesis, focal adhesion, melanogenesis, leukocyte transendothelial migration, Wnt signaling, and type 2 diabetes mellitus. According to the different expression levels in the microarrays and their putative functions, six differentially expressed genes (LHCGR, ANGPTL1, TNIK, GRIN2A, SFRP4, and SOCS3) were selected and analyzed by quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Moreover, the molecular signatures (LHCGR, TNIK, and SOCS3) were associated with developmental potential from embryo to blastocyst stage and were proposed as biomarkers of embryo viability in PCOS patients. Our results may be clinically important as they offer a new potential strategy for competent oocyte/embryo selection in PCOS patients.

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Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality

Cited by 181 publications

References 27 publications

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Differences in the transcriptional profiles of human cumulus cells isolated from MI and MII oocytes of patients with polycystic ovary syndrome

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