Studies of energy‐linked reactions: Dihydrolipoate‐ and oleate‐ dependent ATP synthesis in yeast promitochondria

Griffiths, David E.; Hyams, Robert L.; Bertoli, Enrico

doi:10.1016/0014-5793(77)80747-3

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…coli free of any detectable amount of free or bound lipoate. These findings are in contrast with [1][2][3][4][5][6]. In addition the observation of a DBCT-sensitive ATPase activity in these membranes casts some doubts on the proposal that DBCT inhibition is only due to a specific and covalent interaction with membrane-associated lipoic acid.…”

Section: Resultsmentioning

confidence: 84%

“…On the basis of experiments performed in a large variety of energy-transducing systems and on isolated ATPase complex from mitochondria, a fundamental role in the mechanism of membrane-associated ATP synthesis is played by lipoic acid has been suggested, which should provide the link coupling the electrontransport chains and the ATP-synthetase complexes [1][2][3]. According to [1,4] the terminal reactions of phosphorylation in membranous systems involve a cycle of transacylation and transphosphorylation reactions analogous to those involved in substrate level phosphorylation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative phosphorylation and proton translocation in a lipoate‐deficient mutant ofEscherichia coli

et al. 1979

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Oxidative phosphorylation and proton translocation in a lipoate‐deficient mutant ofEscherichia coli

et al. 1979

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“…Much reduced or no activity in mitochondrial membranes with highly saturated fatty acyl compositions has been documented for cytochromes a, a3, b and c, mitochondrial ATPase, succinate oxidase and NADH oxidase (45)(46)(47). Fatty acids apparently mediate many mitochondrial functions including the rate of proteolysis of mitochondrial translation prod ucts (48), dihydrolipoate-induced ATP synthesis in promitochondria (49), mitochondrial proliferation (50) and release from glucose re pression (51). The Δ-9 isomer was the most efficient of the cisoctadecenoic acid compounds evaluated in this latter study, with eicosaenoic acid supporting 15% of the release induced by oleic acid.…”

Section: Enzymic Regulationmentioning

confidence: 99%

Fatty Acids and Fungal Development: Structure-Activity Relationships

Kerwin

1987

ACS Symposium Series

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Free and esterified fatty acids and their metabolites regulate fungal morphogenesis in diverse and poorly understood ways. Through use of structure-function relationships, the physiological bases for morphological development in fungi can be directly or indirectly investigated. Use of simple categories for classification, e.g. saturated vs. unsaturated or preferential partitioning in fluid or gel phases, can be misleading due to the presence of segregated lipid domains in cell membranes or to selective metabolism. Fatty acid composition has been shown to regulate a variety of membrane transport processes, oxidative phosphorylation, and a number of transferases and other enzymes. Fungal growth requirements for fatty acids can vary among genera, species or even isolates of the same species. Sporulation and spore germination are also affected by fatty acid composition as are the interactions between fungal parasites and their plant or insect hosts. Preliminary investigations support a role for oxygenated fatty acid derivatives in fungal morphogenesis.Fatty acids, which are integral components of cellular and organelle membranes, primary storage products and substrates for secondary metabolism, are involved in the regulation of fungal morphogenesis. Changes in fatty acid composition and metabolism associated with fungal growth and differentiation (reviewed in 1-6) have been extensively documented; although alterations in fatty acid components have been associated with the development of fungi, specific structurefunction relationships have rarely been established. Secondary metabolism of these compounds has also largely been ignored, especially concerning possible regulatory roles of e.g. oxidized fatty acids in fungi. The following review focuses on studies which attempt to more precisely relate fatty acid structure to physiological and morphological development in fungi, often in terms of their physical properties. Initial observations are also presented concerning potential regulatory roles for fatty acid cyclooxygenase and lipoxygenase products in fungal systems.

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“…Moreover, the fact that TFo has no SH group proves the idea that SH groups or lipoate do not participate in the functioning of TFo. Although lipoate [13] and SH [35] have been reported to be involved in ATP synthesis of other biomembranes, the considerable homology in the functioning portion of one of the subunits of mesophilic Fo's and TFo [47] suggests that these proteins have the same mechanism of H "-translocation.…”

Section: Urea (M)mentioning

confidence: 99%

“…Thus the widely accepted idea that unsaturation of the lipids is essential for fluidity and functioning of biomembranes is not appli--cable to thermophiles. The possibility of energy transfer through oleic acid and lipoic acid [13] in the membrane is also excluded by the fact that neither was detected in reconstituted H +-ATPase liposomes [23,30].…”

Section: Thermostable Lipidsmentioning

confidence: 99%

Energy-transducing proteins in thermophilic biomembranes

Kagawa

1980

J. Membrain Biol.

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Biomembranes are the major site of energy transduction. The chemisomotic theroy of energy transduction is based on the following four major systems (i) H+-ATPase which is composed of a catalytic portion (F1) and a H+-channel (Fo), (ii) electron transport components, (iii) H+-linked porters, and (iv) a H+-impermeable lipid bilayer which is plugged through by systems i to iii that are specially oriented to translocate H+. Studies on the molecular mechanism of energy transduction have been hampered by the impurity, instability and complexity of preparations of membrane proteins from mesophilic organism. However, using stable, simple membrane proteins from a thermophilic bacterium, we obtained the following results: 1) Thermophilic H+-ATPase was dissociated into 5 subunits of F1 and 3 subunits of Fo and their functions and structures were studied by reconstitution. F1 was crystallized. 2) Thermophilic cytochrome oxidase, cytochrome c and NADH-dehydrogenase were purified. In contrast to the complex mitochondrial cytochrome oxidase (7 subunits) and NADH-dehydrogenase (3 subunits), the purified thermophilic proteins were shown to be composed of single components. 3) H+-linked porters such as a H+-driven amino acid carrier and a Na+-H+ antiporter were characterized. 4) Thermophilic lipids were shown to be completely saturated. Using these stable lipids, liposomes capable of H+-driven vectorial reactions including net ATP synthesis and alanine transport were reconstituted.

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Studies of energy‐linked reactions: Dihydrolipoate‐ and oleate‐ dependent ATP synthesis in yeast promitochondria

Cited by 15 publications

References 14 publications

Oxidative phosphorylation and proton translocation in a lipoate‐deficient mutant ofEscherichia coli

Oxidative phosphorylation and proton translocation in a lipoate‐deficient mutant ofEscherichia coli

Fatty Acids and Fungal Development: Structure-Activity Relationships

Energy-transducing proteins in thermophilic biomembranes

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