Studies of Cryoproteins in Systemic Lupus Erythematosus*

Hanauer, Lonnie B.; Christian, Charles L.

doi:10.1172/jci105541

Cited by 108 publications

(37 citation statements)

References 17 publications

(14 reference statements)

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“…Cryoprecipitates from patients with SLE have been shown to contain Clq as well as rheumatoid factor (12). This observation was extended by the demonstration of Clq precipitins in serum and the specific concentration of Clq in cryoprecipitates over serum levels (13 …”

Section: Discussionmentioning

confidence: 95%

“…This strongly suggests that the observed titers for the polynucleotide antibodies, which were relatively much higher in the cryoprecipitates than in the serums, were not due to noncryoprecipitable serum immunoglobulin contamination of the cryoprecipitates. Previous investigation has shown IgM anti-IgG to be a significant component of the cryoprecipitates in SLE (12)(13)(14) and to be enriched relative to the serum level (13,14). The present data concerning the presence of antipolynucleotide-polynucleotide complexes in cryoprecipitates suggest the strong possibility that, in serum, IgM rheumatoid factor interacts with such complexes, thereby promoting their precipitation in the cold.…”

Section: Discussionmentioning

confidence: 99%

“…In most of these clinical or experimental settings, intermittent antigenemia has been identified or suspected. IgM rheumatoid factor is often present and comprises varying proportions of the cryoprotein depending on the disorder involved (8,9,(12)(13)(14). Cryoprecipitation also appears to involve fixation of Clq in certain sera of patients with systemic lupus erythematosus (SLE)1 (12,13).…”

Section: Introductionmentioning

confidence: 99%

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Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus.

Winfield¹,

Koffler²,

Kunkel³

1975

J. Clin. Invest.

113

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A B S T R A C T Although the association of cryoglobulinemia with hypocomplementemia and tissue injury in systemic lupus erythematosus is well recognized, the composition of cryoprecipitates in terms of circulating antigens and antibodies in this disease is less clear. To clarify this question, cryoprecipitates from patients with SLE were examined with sensitive assay techniques for certain antipolynucleotide antibodies and DNA antigen. DNA antibodies were highly enriched relative to serum levels in the majority of cryoprecipitates. DNA antigen was also demonstrable. Antibody to ribonucleoprotein, although less frequently present, was similarly enriched in certain cryoprecipitates. In contrast, antidouble strand RNA, which was commonly detectable in relatively high titer in serum, was only minimally concentrated in a minority of cryoprecipitates. Absorption experiments using red blood cells heavily coated with polynucleotide antigen indicated that a major proportion of the IgG in certain cryoprecipitates was specific antibody.The data strongly suggest that the cryoprecipitates in systemic lupus erythematosus represent circulating immune complexes that are soluble at 370C and come out of solution in the cold. The marked concentration of immune complexes in the cryoglobulin offers a simple and direct method -for determination of the nature of the complexes. The accumulated evidence obtained in the present study indicates that these complexes closely reflect, in their composition, the circulating immune complexes which are most significant pathogenetically in renal tissue injury.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus.

Winfield¹,

Koffler²,

Kunkel³

1975

J. Clin. Invest.

113

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“…However, the nature of the suspected antigens is largely unknown. Attempts to identify possible antigens have centered about a variety of drugs, especially in patients with vasculitis (28)(29)(30); DNA in lupus nephritis (31)(32)(33), and IgG especially in rheumatoid arthritis and in illnesses accompanied by "mixed" (IgM and IgG) cryoproteins (34)(35)(36)(37)(38)(39). If IgG were playing such a role in these patients, either in its native, unaltered state or altered so as to react with rheumatoid factor, it would provide a mechanism whereby large quantities of potential antigen would be available to produce tissue damage.…”

Section: Discussionmentioning

confidence: 99%

Hypercatabolism of normal IgG; an unexplained immunoglobulin abnormality in the connective tissue diseases

Wochner¹

1970

J. Clin. Invest.

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A BS TRA CT The metabolism of radioiodinated IgG was studied in a series of 42 patients with connective tissue diseases (16 systemic lupus erythematosus, nine rheumatoid arthritis, five polymyositis, five vasculitis, and seven miscellaneous diagnoses). Fractional catabolic rates were increased and survival half-lives were shortened in all diagnostic categories indicating hypercatabolism of IgG. This hypercatabolism was masked by increased IgG synthesis, resulting in elevated serum concentrations of IgG in patients with systemic lupus erythematosus and rheumatoid arthritis and in generally normal concentrations in the others.The metabolism of iodinated IgM was also studied in eight patients with systemic lupus erythematosus, in seven with rheumatoid arthritis, and in 12 controls. The fractional catabolic rates were normal in both groups of patients. Serum concentrations of both IgM and IgA were moderately elevated in all diagnostic categories. Serum albumin metabolism was entirely normal in the nine subjects studied who were not receiving corticosteroids; in three who were receiving them, moderate hypercatabolism was observed.The hypercatabolism of IgG could not be accounted for by factors previously known to alter IgG metabolism. It was not observed in 15 patients with other chronic, inflammatory diseases and was not explained by concomitant administration of adrenal corticosteroids to some patients. Identical results were obtained whether the IgG was obtained from a patient himself or from a normal donor, demonstrating that the hypercatabolism is a host defect and not an abnormality of the protein.

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“…Attempts to eluate antiglobulins from kidneys have also met with a low incidence of success (36,37), except in some patients with SLE and cryoglobinemia (13)(14)(15)38 Although these data suggest that antiglobulins are found more frequently in the tissues and sera of patients with relatively severe glomerular injury, they do not necessarily indicate that antiglobulins influence the inflammatory response within the glomeruli. In experimental animals, human antiglobulins are not directly nephrotoxic (16).…”

Section: Serologic Techniquesmentioning

confidence: 99%

Antiglobulins and glomerulonephritis. Classification of patients by the reactivity of their sera and renal tissue with aggregated and native human IgG.

Rossen¹,

Reisberg²,

Sharp³

et al. 1975

J. Clin. Invest.

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Studies of Cryoproteins in Systemic Lupus Erythematosus*

Cited by 108 publications

References 17 publications

Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus.

Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus.

Hypercatabolism of normal IgG; an unexplained immunoglobulin abnormality in the connective tissue diseases

Antiglobulins and glomerulonephritis. Classification of patients by the reactivity of their sera and renal tissue with aggregated and native human IgG.

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