Studies of Contact Hypersensitivity Induction in Mice with Optimal Sensitizing Doses of Hapten

Kurimoto, Iwao; Streilein, J. Wayne

doi:10.1111/1523-1747.ep12363616

Cited by 40 publications

(32 citation statements)

References 14 publications

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“…25 l (1.5 g) of 0.5% DNFB in 4:1 acetone/olive oil (Sigma Chemical Pty. Ltd., Sydney, Australia) was applied onto the shaved abdomen or footpads of mice on days 0 and 1, according to the previously described optimal dosage for these mouse strains (36). Control animals were painted with acetone/olive oil (vehicle control).…”

Section: Methodsmentioning

confidence: 99%

Mast cell activation and migration to lymph nodes during induction of an immune response in mice.

Wang¹,

Tedla²,

Lloyd³

et al. 1998

J. Clin. Invest.

158

114

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The mast cell response in skin and lymph nodes was examined during the sensitization phase of dinitrofluorobenzene (DNFB)-induced contact hypersensitivity in mice. Degranulation of 62% of mast cells in DNFB-exposed skin was evident within 30 min of a dual application of DNFB, reaching a peak of 77% at 24 h, and persisting in 42% after 5 d. Abundant expression of macrophage inflammatory protein (MIP)-1 ␣ and MIP-1 ␤ mRNAs and proteins was observed in keratinocytes, and mast cell degranulation was significantly inhibited after administration of neutralizing antibodies to MIP-1 ␣ , but not MIP-1 ␤ . During DNFB sensitization, the mast cell density in the skin decreased by half, concurrent with a fivefold expansion of mast cell numbers in draining lymph nodes. Fluorescent-labeled mast cells injected into the skin appeared in draining lymph nodes after application of DNFB, followed by subsequent migration to the spleen. In lymph nodes, mast cells were an abundant and predominant source of MIP-1 ␤ , neutralization of which partially inhibited T lymphocyte recruitment. These results indicate that mast cells contribute to the induction of this primary immune response by activation at and migration from the site of antigen encounter to draining lymph nodes, wherein they mediate T lymphocyte recruitment by production of MIP-1 ␤ . ( J. Clin. Invest. 1998. 102:1617-1626.)

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Section: Methodsmentioning

confidence: 99%

Mast cell activation and migration to lymph nodes during induction of an immune response in mice.

Wang¹,

Tedla²,

Lloyd³

et al. 1998

J. Clin. Invest.

158

114

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“…[52][53][54] In brief, DNFB (Sigma Chemical Co., St. Louis, MO) was applied epicutaneously to shaved abdominal skin (50 l). This induced cutaneous inflammation at the application site within 48 hours.…”

Section: Induction Of Inflammation In Skinmentioning

confidence: 99%

Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

Hamrah

Chen

Zhang

et al. 2003

The American Journal of Pathology

123

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“…Neither routine histological analysis of internal organs nor staining for IgG deposition in the kidney of aged mice revealed signs of spontaneous autoimmune disease (data not shown), which led us to challenge T cell-specific Ga 12 ,Ga 13 double-deficient mice in two models of T cell-mediated pathology: contact hypersensitivity to dinitrofluorobenzene (DNFB) as a model for responses to foreign antigen (Kurimoto and Streilein, 1993) and low-dose streptozotocin-induced diabetes mellitus as a model of autoimmune disease (Like and Rossini, 1976).…”

Section: Immune Functions In Vivomentioning

confidence: 99%

“…The resulting activation of naive T cells and generation of effector cells leads during the elicitation phase (rechallenge with DNFB in the ear of the skin) to leukocyte recruitment to the ear, resulting in a Th1-cell-dependent local inflammation with consecutive swelling (Kurimoto and Streilein, 1993). We found that T cell-specific Ga 12 ,Ga 13 double-deficient mice showed increased ear swelling at 24, 48, and 72 hr after sensitization and challenge ( Figure 7A), and histological analyses of ear sections taken at 72 hr showed an increased leukocytic infiltration (data not shown).…”

Section: Immune Functions In Vivomentioning

confidence: 99%

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Guanine Nucleotide-Binding Proteins of the G12 Family Shape Immune Functions by Controlling CD4+ T Cell Adhesiveness and Motility

et al. 2009

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Integrin-mediated adhesion plays a central role in T cell trafficking and activation. Genetic inactivation of the guanine nucleotide-binding (G) protein alpha-subunits Galpha(12) and Galpha(13) resulted in an increased activity of integrin leukocyte-function-antigen-1 in murine CD4(+) T cells. The interaction with allogeneic dendritic cells was enhanced, leading to an abnormal proliferative response in vitro. In vivo, T cell-specific inactivation of Galpha(12) and Galpha(13) caused lymphadenopathy due to increased lymph node entry and enhanced T cell proliferation, and the susceptibility toward T cell-mediated diseases was enhanced. Mechanistically, we show that in the absence of Galpha(12) and Galpha(13) the activity of the small GTPases Rac1 and Rap1 was increased, whereas signaling of the small GTPase RhoA was strongly reduced. Our data indicate that locally produced mediators signal through Galpha(12)- and Galpha(13)-coupled receptors to negatively regulate cell polarization and adhesiveness, thereby fine-tuning T cell trafficking, proliferation, and susceptibility toward T cell-mediated diseases.

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Studies of Contact Hypersensitivity Induction in Mice with Optimal Sensitizing Doses of Hapten

Cited by 40 publications

References 14 publications

Mast cell activation and migration to lymph nodes during induction of an immune response in mice.

Mast cell activation and migration to lymph nodes during induction of an immune response in mice.

Novel Expression of Vascular Endothelial Growth Factor Receptor (VEGFR)-3 and VEGF-C on Corneal Dendritic Cells

Guanine Nucleotide-Binding Proteins of the G12 Family Shape Immune Functions by Controlling CD4+ T Cell Adhesiveness and Motility

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