Studies of cloned sequences from four drosophila heat shock loci

Holmgren, Robert A.; Livak, Kenneth J.; Morimoto, Richard I.; Freund, Robert; Meselson, Matthew

doi:10.1016/0092-8674(79)90246-0

Cited by 198 publications

(127 citation statements)

References 23 publications

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“…However, a short leader sequence could be missed by these methods. S1 mapping and DNA sequencing of a region preceding the 5 Figure 2.…”

Section: Resultsmentioning

confidence: 99%

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Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation

O’Connor

Lis

1981

Nucl Acids Res

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We report the isolation and characterization of a cloned DNA of D. melanogaster, Dm4L, that is derived from the major heat shock puff site at 63B. This segment contains two closely linked genes that are each present once per Drosophila haploid genome. One of these, the hsp 83 gene, encodes an abundant heat shock mRNA that, unlike other major heat shock mRNAs, is also abundant in uninduced (230) Kco cells. Although only a slight increase in the level of total hsp 83 RNA can be detected after heat shock in Kco cells, the level of hsp 83 poly(A)+ mRNA increases more than 6-fold and the level of pulse-labeled hsp 83 RNA in total cellular RNA increases 11-fold relative to uninduced cells. In contrast, the levels of total, poly(A) , and pulselabeled RNA homologous to the second gene, 63B-T2, are approximately the same in both induced and uninduced cells. Hence, even though these genes are separated by only one thousand base pairs, and, from in situ hybridization to polytene chromosomes, both lie within the heat shock puff, they display strikingly different regulatory properties. These results demonstrate that close linkage of a gene to a heat shock puff is not sufficient to render its expression heat inducible.

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“…However, a short leader sequence could be missed by these methods. S1 mapping and DNA sequencing of a region preceding the 5 Figure 2.…”

Section: Resultsmentioning

confidence: 99%

“…One gene encodes the hsp 83 protein (4,5). This protein is abundant in uninduced cells and increases in heat shock induced cells (6,7).…”

Section: Introductionmentioning

confidence: 99%

Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation

O’Connor

Lis

1981

Nucl Acids Res

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“…Seventh, hsp83 mRNA is found in uninduced immature ovaries and early egg chambers, whereas other heat shock mRNAs are not detected (10). Finally, the hsp83 gene is the only heat shock gene known to contain an intervening sequence (2). Together, these observations indicate that expression of the hsp83 gene involves regulatory events at the levels of transcription, processing, and translation that are not shared by other heat shock genes.…”

Section: Introductionmentioning

confidence: 86%

“…Most genes active at 23°C are repressed while a specific set of heat shock genes is activated both at the transcriptional and translational levels (1). One of these induced genes, which encodes the 83,000 d heat shock polypeptide (hsp83), is a single copy gene located at the 63B cytogenetic locus of D. melanogaster (2). Several lines of evidence suggest that the expression of the hsp83 gene differs significantly from the other major heat shock genes of D. melanogaster.…”

Section: Introductionmentioning

confidence: 99%

Localization of the hsp83 transcript within a 3292 nucleotide sequence from the 63B heat shock locus ofD. melanogaster

Hackett¹,

Lis²

1983

Nucl Acids Res

109

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We have determined the complete nucleotide sequence of a 3292 bp cloned segment derived from the 63B heat shock cytogenetic locus of D. melanogaster. Within this segment we have positioned the start of transcription and RNA splice sites of the unique gene that encodes the 83,000 d heat shock polypeptide (hsp83 gene) by SI mapping and synthesis of cDNA from restriction fragment primed mRNA. The sequence begins at a point 879 bp upstream from the transcription start and includes the 149 bp nontranslated first exon, the 1139 bp intron and extends 1125 bp into the protein coding region. These data identify a single open translation reading frame for the first 375 amino acids of the 83,000 d polypeptide, beginning with the first ATG codon located at the 3' intron-exon junction. We discuss and demonstrate the use of E. coli exonuclease III generated single-strand DNA probes as an alternative to strand separation for S1 mapping of mRNA. We also use homology search criteria based upon known protein-DNA binding sites to compare our hsp83 sequence with other sequenced Drosophila heat shock genes. These comparisons indicate that a large region of approximately 80 bp centered around the transcription initiation point of the hsp83 gene shares only a 31% homology with the corresponding region of the hsp70 gene, whereas the hsp22, 23, 26, and 27 genes share a 54% homology with hsp70 in this region. The lower homology of the hsp83 gene is consistent with the deviant nature of this heat shock gene.

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“…However, more recent studies have reported the presence of four introns in Hsp70 gene of the filamentous fungi N. crassa (Kapoor et al, 1995) and one intron in aquatic fungus Blastocladiella emersonii (Stefani and Gomes, 1995). Introns have been also found in Hsp83 of Drosophila (Holmgren et al, 1979) and Hsp70 of Caenorhabditis elegans (Snutch et al, 1988). The Hsp88 genomic DNAs from P. tenuipes Jocheon-1, P. tenuipes and C. militaris all contain 5 introns and 6 exons with the length of 13,62,32,1,438,306,288 bp, encoding 713 amino acid residues.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, P. tenuipes, Cordyceps militaris, and C. pruinosa

Liu¹,

Park²,

Kim³

et al. 2012

International Journal of Industrial Entomology

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Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain), P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288 nucleotides encoding 713 amino acid residues, whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288 nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600 nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.

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Studies of cloned sequences from four drosophila heat shock loci

Cited by 198 publications

References 23 publications

Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation

Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation

Localization of the hsp83 transcript within a 3292 nucleotide sequence from the 63B heat shock locus ofD. melanogaster

Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, P. tenuipes, Cordyceps militaris, and C. pruinosa

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