Studies of chitosan/pectin complexes exposed to UV radiation

Kowalonek, Jolanta

doi:10.1016/j.ijbiomac.2017.05.081

Cited by 47 publications

(27 citation statements)

References 51 publications

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“…In the region of 1900-1000 cm −1 characteristic peaks of CvO, symmetric and asymmetric COO − , and COC stretching appeared as typical vibrational peaks of pectin. 55,56 For the pectin-g-PCL sample, the presence of the characteristic peaks of pectin confirmed the non-degradation of this polymer during the quenching process. Moreover, the peak corresponding to CvO stretching of PCL at 1729 cm −1 (ref.…”

Section: Synthesis and Structural Analysis Of Pectin-g-pclmentioning

confidence: 69%

Interpenetrating network gelatin methacryloyl (GelMA) and pectin-g-PCL hydrogels with tunable properties for tissue engineering

et al. 2018

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The design of new hydrogel-based biomaterials with tunable physical and biological properties is essential for the advancement of applications related to tissue engineering and regenerative medicine. For instance, interpenetrating polymer network (IPN) and semi-IPN hydrogels have been widely explored to engineer functional tissues due to their characteristic microstructural and mechanical properties. Here, we engineered IPN and semi-IPN hydrogels comprised of a tough pectin grafted polycaprolactone (pectin-g-PCL) component to provide mechanical stability, and a highly cytocompatible gelatin methacryloyl (GelMA) component to support cellular growth and proliferation. IPN hydrogels were formed by calcium ion (Ca2+)-crosslinking of pectin-g-PCL chains, followed by photocrosslinking of the GelMA precursor. Conversely, semi-IPN networks were formed by photocrosslinking of the pectin-g-PCL and GelMA mixture, in the absence of Ca2+ crosslinking. IPN and semi-IPN hydrogels synthesized with varying ratios of pectin-g-PCL to GelMA, with and without Ca2+-crosslinking, exhibited a broad range of mechanical properties. For semi-IPN hydrogels, the aggregation of microcrystalline cores led to formation of hydrogels with compressive moduli ranging from 3.1 to 10.4 kPa. For IPN hydrogels, the mechanistic optimization of pectin-g-PCL, GelMA, and Ca2+ concentrations resulted in hydrogels with comparatively higher compressive modulus, in the range of 39 kPa-5029 kPa. Our results also showed that IPN hydrogels were cytocompatible in vitro and could support the growth of three-dimensionally (3D) encapsulated MC3T3-E1 preosteoblasts in vitro. The simplicity, technical feasibility, low cost, tunable mechanical properties, and cytocompatibility of the engineered semi-IPN and IPN hydrogels highlight their potential for different tissue engineering and biomedical applications.

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Section: Synthesis and Structural Analysis Of Pectin-g-pclmentioning

confidence: 69%

Interpenetrating network gelatin methacryloyl (GelMA) and pectin-g-PCL hydrogels with tunable properties for tissue engineering

et al. 2018

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“…After the formation of fragments of chitosan during the depolymerization reaction, a cross-linking can be produced by linking of amine groups to pyranose ring. The good water and thermal stabilities of these polymeric materials after UV exposure may be explained by cross-linking after the formation of free radicals [52], which can cause the molecular rearrangement by absorbed energy. Based on the identification of the functional groups which take part, the shifting of the absorptions bands at 1655 and 1564 cm −1 corresponding to C=O and NH 2 , respectively (Figures 4(b) and 4(c)), might confirm the structural changes as a result of these possible mechanisms [34].…”

Section: Ft-ir Analysismentioning

confidence: 99%

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Aguirre-Chagala

Pavón-Pérez

Altúzar

et al. 2017

Journal of Nanomaterials

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Chitosan membranes are widely applied for tissue engineering; however, a major drawback is their low resistance in aqueous phases and therefore the structure collapses impeding their long-term use. Although there is extensive research, because of chitosan’s importance as a biomaterial, studies involving chitosan-based membranes are still needed. Herein, a detailed investigation of diverse chemical routes to cross-link fibers in situ by electrospinning process is described. In case of using genipin as cross-linker, a close relationship with the content and the mean diameter values is reported, suggesting a crucial effect over the design of nanostructures. Also, the physical resistance is enhanced for the combination of two types of methods, such as chemical and physical methods. Cross-linked fibers upon exposure to long wave ultraviolet A (UVA light) change their morphology, but not their chemical composition. When they are incubated in aqueous phase for 70 days, they show an extensive improvement of their macrostructural integrity which makes them attractive candidates for tissue engineering application. As a result, the thermal properties of these materials reveal less crystallinity and higher temperature of degradation.

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“…1400 cm − 1 attributed to asymmetric and symmetric stretching vibrations of carboxylate anions can be distinguished, respectively. That band can be overlapped with -CH 2 scissoring vibrations band [60]. On the other hand, the (amide I) band should be hardly visible [61].…”

Section: Ftirmentioning

confidence: 97%

“…However, with the pH increase, the interaction becomes more electrostatic [53]. The bands at 1380-1383 , 1318-1325 , and 1251-1255 cm − 1 can be assigned to the deformation vibrations of methyl group [53], -CH wagging, -CH 2 twisting vibrations, [60] respectively. The last one and some of the bands described below, cannot be distinguished in the spectra of silica-containing composites, because of a broad band, which can be attributed to longitudinal optic Si-O-Si asymmetric (1170 cm − 1 ) and asymmetric stretching (1070 cm − 1 ) band [65] overlapping with it.…”

Section: Ftirmentioning

confidence: 99%

Hierarchical porous carbon templated with silica spheres of a diameter of 14 nm from pure chitosan or a chitosan/ZnCl2 solution

2018

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Nitrogen-containing mesoporous carbons with the use of colloidal silica spheres of (14 nm) and chitosan as a carbon precursor were obtained. A removal of such small template particles from carbonized silica-chitosan composite is difficult and HF with a minimum concentration of 15 wt% should be used. By varying the silica-to-chitosan ratio, the porous characteristic of products is controlled. The modification by ZnCl 2 with a molar Zn-to-C (in chitosan mass) ratio of '6' results in the development of microporosity; however it is accompanied by a significant reduction of mesopore volume (V mes ). The addition of ZnCl 2 in a ratio of '5.25' and pH adjustment to 5.8 increase the volumes of micropores, small mesopores, BET surface area to 1975 m 2 /g, and preserve V mes of 4.15 cm 3 /g. The novelty of the presented strategy is the creation of microporosity in the hard-templated materials by incorporating ZnCl 2 into the mixture of Ludox HS-40 template and chitosan precursor, as well as the investigation on how the pH of synthesis influences the final porosity. The pH of a silica-chitosan-zinc solution, equal to 3.9, provides some coordination of Zn 2+ by -OH and -NH 2 groups, whereas pH adjustment to 5.8 results in the precipitation of a new template-Zn(OH) 2 .

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Studies of chitosan/pectin complexes exposed to UV radiation

Cited by 47 publications

References 51 publications

Interpenetrating network gelatin methacryloyl (GelMA) and pectin-g-PCL hydrogels with tunable properties for tissue engineering

Interpenetrating network gelatin methacryloyl (GelMA) and pectin-g-PCL hydrogels with tunable properties for tissue engineering

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Hierarchical porous carbon templated with silica spheres of a diameter of 14 nm from pure chitosan or a chitosan/ZnCl2 solution

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