Studies of cannabinoid metabolites — a free radical immunoassay

Cais, Michael; Dani, S.; Josephy, Y.; Modiano, A.

doi:10.1016/0014-5793(75)81005-2

Cited by 21 publications

(3 citation statements)

References 9 publications

(12 reference statements)

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“…Synthesis of Vanadophosphoric Acid. This acid was prepared by Wu's method (7) by dissolving 100 g of sodium metavanadate in 450 ml of water adding 15 cm3 of 85% H3PO4 and 80 cm3 of coned. HC1 and boiling for 8 h, with a return condenser.…”

Section: Resultsmentioning

confidence: 99%

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Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Vinson¹,

Patel²,

Patel³

1977

Anal. Chem.

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A thin-layer chromatographic method Is described for the detection of A9-tetrahydrocannabinol in blood and serum. The procedure utilizes a reagent, 2-p-chlorosulfophenyl-3-phenylindone, which reacts with the phenolic group to form a derivative. Following extraction and cleanup, the derivative Is prepared and separated from reagent and naturally occurring compounds by thin-layer chromatography. The derivative is then detected by spraying the thin-layer plate with an alkoxide spray which produces a fluorescent spot visible under long wave ultraviolet light. The method can detect 0.2 ng/ml of tetrahydrocannabinol in 5 ml of serum and is suitable for routine screening.

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Section: Resultsmentioning

confidence: 99%

“…A promising approach to determining THC is the development of immunoassay techniques. A free radical immunoassay method has been developed for THC and its metabolites in urine (7). Another group has used radioimmunoassay for THC in plasma and urine (8).…”

mentioning

confidence: 99%

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Vinson¹,

Patel²,

Patel³

1977

Anal. Chem.

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“…al., 1995), CBDA synthase (Taura et al, 1996) and CBCA synthase (Morimoto et al, 1997). An immunological approach for assaying small quantities of THC and its isomers in body fluids and cell preparation have been investigated in many groups (Gross et al, 1974;Teale et al, 1974; 1975; Cais et al, 1975). However, since no production of MAb against cannabinoid acids has been published yet, we wish to summarize the preparation and determination of the THCA-carrier protein conjugate by MALDI tof mass spectrometry (Goto et al, 1994), the formation of MAb against cannabinoids and an immunoassay system for the detection of THCA using a MAb (Tanaka et al, 1996), and an application for biotransformation of CBN, CBD and CBDA using plant tissue culture (Tanaka et al, 1996).…”

Section: Production Of Mab Against Marihuana Compounds (Cannabinoids)mentioning

confidence: 99%

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Shoyama¹,

Tanaka²,

Fukuda³

1999

Cytotechnology

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The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myeloma cells. The cross-reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.6%. A very small cross-reaction appeared with other derivatives. The full measuring range of the assay extends from 5 ng to 5 mug/ml of forskolin. Immunoaffinity column chromatography using anti-forskolin MAbs appears to be far superior to previously published separation methods. The capacity of the immunoaffinity column as determined by ELISA is 9 mug/ml. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii. A MAb against tetrahydrocannabinolic acid (THCA) was produced. The cross-reaction of anti-THCA antibody against other cannabinoids was very wide. Many cannabinoids and a spiro-compound were reactive, but did not react with other phenolics. It became evident that this ELISA was able to be applied to the biotransformation experiments of cannabinoids in plant tissue culture system. Anti-ginsenoside Rb1 MAbs were produced. New western blotting method of determination for ginsenosides was established. Ginsenosides separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO(4) solution followed by BSA, resulting in a ginsenoside-BSA conjugate. Immunostaining of ginsenosides was more sensitive compared to other staining. Immunostaining of ginsenosides in the fresh ginseng root was succeeded using anti-ginsenoside Rb1 (GRb1) MAb after blotting to PVDF membrane.

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Preparation of 2‐[¹²⁵I] iodohistamine‐labelled Δ⁸‐tetrahydrocannabinol‐11‐oic acid for use in cannabinoid radioimmunoassay

Law

Mason

Moffat

et al. 1982

Labelled Comp Radiopharmac

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A simple method is described for the preparation of 2‐[125I]iodohistamine‐labelled Δ8‐tetrahydrocannabinol‐11‐oic acid with high specific activity for use in radioimmunoassay. This compound is produced in high yield and shows excellent radiochemical stability when stored at 4°C. The radiolabelled cannabinoid has been shown to bind avidly to four different broadly specific cannabinoid antisera. It is the only [125I] labelled cannabinoid suitable for use in radioimmunoassay with such antisera.

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Studies of cannabinoid metabolites — a free radical immunoassay

Cited by 21 publications

References 9 publications

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

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Preparation of 2‐[¹²⁵I] iodohistamine‐labelled Δ⁸‐tetrahydrocannabinol‐11‐oic acid for use in cannabinoid radioimmunoassay

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Studies of cannabinoid metabolites — a free radical immunoassay

Cited by 21 publications

References 9 publications

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Detection of tetrahydrocannabinol in blood and serum using a fluorescent derivative and thin-layer chromatography

Untitled

Preparation of 2‐[125I] iodohistamine‐labelled Δ8‐tetrahydrocannabinol‐11‐oic acid for use in cannabinoid radioimmunoassay

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Preparation of 2‐[¹²⁵I] iodohistamine‐labelled Δ⁸‐tetrahydrocannabinol‐11‐oic acid for use in cannabinoid radioimmunoassay