Studies in the Reaction of Urine

Kaye, Geoffrey

doi:10.1038/icb.1929.21

Cited by 8 publications

(6 citation statements)

References 7 publications

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“…The T cell activation and associated proteins (TAP and TPAa) recognized by these monoclonals were recently shown also to be controlled by genes linked to the Ly-6 locus . Similar transmembrane signalling effects have been noted with monoclonal antibodies specific for Thy-i and the mouse and human T cell receptor and the associated human T3 complex proteins (Gunter et al, 1984;Kaye and Janeway, 1984;Meuer et al, 1984). Biochemical characterization of Ly6C.2 (Takei, 1982), Ly-6E.1 and TAP/TAPa have shown them to be small proteins with Mr 15 000-18 000, apparently distinct from presently described mouse T cell receptor-associated proteins and Thy-I (Campbell et al, 1981;Allison and Lanier, 1985;Samelson et al, 1985a;Oettgen et al, 1986).…”

Section: Introductionsupporting

confidence: 61%

Isolation of a murine Ly-6 cDNA reveals a new multigene family.

LeClair¹,

Palfree²,

Flood³

et al. 1986

The EMBO Journal

115

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The Ly‐6 alloantigens have been shown to play a critical role in T lymphocyte activation. To isolate a Ly‐6 cDNA, synthetic oligonucleotides, based on the partial amino acid sequence of purified Ly‐6E.1 protein, were used to probe a cDNA library. The synthetic oligonucleotides or the isolated cDNA detected a 1.1‐kb RNA species. Sequence analysis of the cDNA clone revealed that the Ly‐6E.1 protein consists of a 26‐amino acid leader followed by a 108‐residue, cysteine‐rich, core protein with no N‐linked glycosylation sites. Southern blot analysis of genomic DNAs revealed multiple bands indicating a family of related genes. Using recombinant inbred and Ly‐6 congenic strains of mice, restriction fragment length polymorphisms were demonstrable, and correlated with the Ly‐6 allotype of the DNA donors. This probe will enable further molecular genetic analysis of the role of Ly‐6‐linked proteins in the process of T lymphocyte activation. Isolation of Ly‐6 genomic clones may promote a further understanding of the complex tissue‐specific expression patterns characteristic of Ly‐6‐linked genes.

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Section: Introductionsupporting

confidence: 61%

Isolation of a murine Ly-6 cDNA reveals a new multigene family.

LeClair¹,

Palfree²,

Flood³

et al. 1986

The EMBO Journal

115

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“…Antibodies, plasmids and other reagents The following monoclonal antibodies were used: 3D3 (anti-D1O TCR, mouse IgGI) (Kaye et al, 1983); C193.5A (anti-D1O TCR, mouse IgGI) (Tite et al, 1986a); F23.1 (anti-Vf38. 1, mouse IgG2a) (Staerz et al, 1985); YCD3-1 (anti-mouse CD3e, rat IgG2c) (Portolds et al, 1989a); GK1.5 (antimouse CD4, rat IgG2b) (Dialynas et al, 1983); YT4.2 (anti-mouse CD4, rat IgG2a) (Janeway Jr et al, 1987); M17/5 (anti-CD lla, rat IgG2a) (Sanchez-Madrid et al, 1982); M1/70 (anti-CDlIb, rat IgG2b) (Springer et al, 1979); M1/9.3 (pan-CD45, rat IgG2a) (Coffman, 1982); and Y-19 (anti-Thy-i, rat IgG) (Jones and Janeway, 1981) were obtained from Dr Charles A.Janeway Jr, Yale University School of Medicine, New Haven, CT. All antibodies were purified from hybridoma supematants by affinity chromatography over protein A -Sepharose columns (Pharmacia).…”

Section: Methodsmentioning

confidence: 99%

“…It was maintained by stimulation every 2 weeks at 5 x 104 cells/ml with mitomycin-C treated H-2k (C3H) spleen cells (5 x 104 cells/ml) plus antigen (100 ug/ml conalbumin) in Click's medium supplemented with 10% inactivated fetal calf serum (culture medium). D10.G4.1 (Kaye et al, 1983) was maintained as described for SR.D10 except that 105 responder cells/mil and 106 spleen cells/mil were used. The I-Ak transfected TAK lymphoma (Buerstedde et al, 1988) was obtained from Dr Charles A.Janeway, Jr. CD4+ T lymphocytes were obtained from spleen cell suspensions of C3H mice.…”

Section: Methodsmentioning

confidence: 99%

Genetic and immunochemical evidence for CD4-dependent association of p56lck with the alpha beta T-cell receptor (TCR): regulation of TCR-induced activation.

et al. 1994

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Recent observations suggest that the tyrosine kinase p56lck is involved in the transduction of transmembrane signals through the antigen specific T cell receptor (TCR) in CD4+ T cells. By means of in vitro kinase assays, we have found that p56lck coprecipitated with the TCR from lysates of a murine CD4+ T cell line in the absence of TCR‐mediated stimuli. Analysis of CD4‐ mutants and CD4‐transfected cells shows that p56lck‐TCR association occurred only when CD4 was present. The functional importance of CD4:p56lck‐TCR association was demonstrated by low activating potential of rare clonotypic antibodies which did not coprecipitate CD4:p56lck, as well as by total or partial loss of anti‐TCR or antigen induced stimulation in CD4‐ cells, which could be recovered by CD4 transfection. Complementation assays using different anti‐TCR antibodies suggest that cross linking of TCR‐p56lck:CD4 plus structural changes in the complex are needed for efficient transduction of activating signals through the TCR in these cells.

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“…The recipients used for transfection were resting D10.G4.1 cells (10-14 days post-antigen stimulation), a murine TH2 clone with specificity for conalbumin on H2Ak (Kaye et al, 1983) and 58a-pcells, a T cell hybridoma lacking an a[B receptor (Letoumeur and Malissen, 1989).…”

Section: Transfection and Cell Culturementioning

confidence: 99%

Predicted complementarity determining regions of the T cell antigen receptor determine antigen specificity.

et al. 1995

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The antigen receptor on T cells (TCR) has been predicted to have a structure similar to a membrane‐anchored form of an immunoglobulin F(ab) fragment. Virtually all of the conserved amino acids that are important for inter‐ and intramolecular interactions in the VH‐VL pair are also conserved in the TCR V alpha and V beta chains. A molecular model of the TCR has been constructed by homology and we have used the information from this, as well as the earlier structural predictions of others, to study the basis for specificity. Specifically, regions of a TCR cloned from an antigen‐specific T cell were stitched into the corresponding framework of a second TCR. Results indicate that the substitution of amino acid sequences corresponding to the complementarity determining regions (CDRs) of immunoglobulin can convey the specificity for antigen and major histocompatibility complex molecules. These data are consistent with a role, but not an exclusive role, for CDR3 in antigen peptide recognition.

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Studies in the Reaction of Urine

Cited by 8 publications

References 7 publications

Isolation of a murine Ly-6 cDNA reveals a new multigene family.

Isolation of a murine Ly-6 cDNA reveals a new multigene family.

Genetic and immunochemical evidence for CD4-dependent association of p56lck with the alpha beta T-cell receptor (TCR): regulation of TCR-induced activation.

Predicted complementarity determining regions of the T cell antigen receptor determine antigen specificity.

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