Studies in Intravascular Coagulation

Abstract

A method has been previously described whereby the infusion of canine serun and serum fractions rich in convertin, but essentially devoid of thromboplastin, prothrombin, ac-globulin and thrombin, initiates venous thrombosis in dogs at sites of partial or complete venous stasis far removed from the site of infusion. It has been suggested that the pathogenesis of venous thrombosis in various clinical states may be referable to local stasis combined with a transient increase in circulating convertin activity. The present study demonstrates that heparin is superior to dicumarol in inhibiting the evolution of such serum-induced intravascular thrombi in dogs.

Comparison of Streptokinase and Heparin in Treatment of Isolated Acute Massive Pulmonary Embolism

“…In accepting this conclusion it is important to question how far heparin was correctly administered. Under experimental conditions the optimum dose of heparin seems to be that which keeps the clotting time in excess of twice the control value at all times (Wessler and Morris, 1955), and that was our policy. The dose required to achieve this varies considerably.…”

The Role of Human Coagulation Factors in Serum-Induced Thrombosis *

“…The data indicate that it is related, directly or indirectly, to at least three first phase coagulation factors (Factor IX, Hageman and PTA) and possibly to the complex evolved during coagulation and characterized as "intrinsic thromboplastin." Moreover, STA activity is distinct from tissue thromboplastin (5), thrombin (5,6), and prothrombin (5, 6) as well as from Factor V, Factor VII, Factor VIII and Factor X. STA activity may have a specific and significant role in the development of intravascular thrombosis in man. Elucidation of this hypothesis must await biochemical studies directed toward its isolation, purification and characterization.…”

“…It is based on the finding that the systemic infusion of serum, in contrast to plasma, rapidly induces massive thrombosis in vascular segments containing stagnant blood far removed from the site of infusion (5). The activity in serum responsible for thrombosis arises during coagulation and is not species-specific.…”

Studies on Clot Promoting Serum Factor (s) (CPSF) -<i>in vitro</i> Experiments-

“…Recently Wessler et al [8][9][10][11][12] made extensive studies on serum-induced thrombosis in experimental animals. Their results certainly gave reassured objective evidence on clot promoting activity of serum both in vivo and in vitro.…”