Studies in Induced Tetraploids of the Family Apocynaceae

Tapadar, N. N. Roy

doi:10.1508/cytologia.28.229

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…The polyploid plant showed morphological characters like stunted growth and presence of larger, thicker and dark green leaves. Such characters have been reported as occurring in induced polyploids by other workers also (Tapadar 1963, Bose and Panigrahy 1969, Biswas and Bhattacharyya 1971, 1972. The deep green colour of the leaves in polyploids has been attributed to the presence of more numer ous and larger chloroplasts (Raghuvanshi and Joshi 1964).…”

Section: Discussionsupporting

confidence: 58%

Morphological and Cytological Studies on a Radiation Induced Polyploid in <i>Capsicum annuum</i> Linn

Indira

Abraham

1977

CYTOLOGIA

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In the course of mutation induction studies on Capsicum annuum Linn., an autotetraploid plant was observed in the M2 population raised from gamma ray irradiated seeds.The present paper deals with morphological and cytological studies on this polyploid. Material and methodsThe variety of Capsicum annuum Linn. used in this study is characterized by attractive purple fruits (Fig. 1). Genetically pure seeds were obtained by selfing for three generations. These were subjected to gamma rays at dose levels ranging from 10 to 15kR. The treated seeds were sown in pots and later transplanted to the field. The resulting M, plants were selfed. Each plant was harvested sepa rately and its progeny (M2) raised in the field. Among the progenies of the 10kR treated plants, one plant was found to be an autotetraploid. Observations on growth, morphological features, flowering and fruit setting of the polyploid were recorded.Cytological studies were carried out from flower buds fixed in 1:3 acetic-alcohol and smeared in acetocarmine.Studies on pollen mitosis were carried out from hematoxylin stained preparations.Pollen sterility was determined from aceto carmine-glycerine mounted slides. Results Morphological studiesThe polyploid was characterized by slower and stunted growth (Fig. 2). The leaves were thicker, broader, large sized (Fig. 3) and deep green in colour as compared to those of the controls. As in other polyploids, the epidermal cells of the palisade and spongy parenchyma as well as the stomata were larger.Flowering was delayed in the polyploid. The flowers borne on the polyploid were conspicuously larger and a general enlargement of the floral structures was noticeable (Fig. 4). Increase in the number of petals and stamens was also observed in many cases. Abnormal flowers showing dissected floral parts or rudimentary structures were also present (Fig. 5).The tetraploid was sterile. Though many flowers were produced, only two fruits were formed. The fruits were much smaller than those of the control plants

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Section: Discussionsupporting

confidence: 58%

Morphological and Cytological Studies on a Radiation Induced Polyploid in <i>Capsicum annuum</i> Linn

Indira

Abraham

1977

CYTOLOGIA

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“…Increase in the size of stomatal guard cells has been observed but the frequency of them was low in comparison to the respective di ploids. Stomatal changes have also been reported in a number colchiploids (Siskovic 1957, Monge et al 1963, Roy Tapadar 1963, Singh 1968, Bose and Pani grahi 1969. Delayed and prolonged duration of flowering have been noticed in the tetraploids.…”

Section: Discussionmentioning

confidence: 69%

Induced Polyploidy in Legumes

Biswas

Bhattacharyya

1972

CYTOLOGIA

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“…Thus, it is revealed that the irregularities of chromosome cannot serve as an only explanation for sterility in case of autotetraploids. Sterility may be due to involvement of same genetic and physiological factors as emphasized by Roy Tapadar (1963). Similar report on F 1 steriles was also available in Fagopyrum inter-specific hybridization (Woo et al 2010).…”

Section: Post-meiotic Studiesmentioning

confidence: 62%

Induced polyploidization in buckwheat (Fagopyrum esculentum Moench)

Srivastava¹,

Kumar

2022

IJGPB

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The significance of polyploidy in the evolutionary process of plants history is well documented. In this concern, seed priming with colchicine as well as the cotton-swab method for polyploidy induction in plants becomes much acquainted in cytogenetic study. The aim of present study was to achieve chromosomal doubling by the application of colchicine to apical meristem of young seedlings. The young seedlings were treated with three different concentrations of colchicine (0.2, 0.4 and 0.6%) for durations of 12, 24 and 36 h each. The colchiploidy with highest proportion approximately 50% with 2n=4x=32 was found at 0.2% concentration for 24 and 36 h. Autotetraploid enduring plants were identified with morphological and cytological variations such as thickness of leaf, larger stomata with low density, larger pollen, large flower etc. Buds were selected from these plants for the cytological study. Cytological analysis including chromosome counting demonstrated that the chromosome number was doubled. The confirmation of autotetraploid plants was achieved by this technique. Tetraploid plants were grown up to maturity and the harvested seeds were sown to establish the second generation which may be used in future breeding programme

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Studies in Induced Tetraploids of the Family Apocynaceae

Cited by 8 publications

References 13 publications

Morphological and Cytological Studies on a Radiation Induced Polyploid in <i>Capsicum annuum</i> Linn

Morphological and Cytological Studies on a Radiation Induced Polyploid in <i>Capsicum annuum</i> Linn

Induced Polyploidy in Legumes

Induced polyploidization in buckwheat (Fagopyrum esculentum Moench)

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