Studies for improved understanding of lipid distributions in human skin by combining stimulated and spontaneous Raman microscopy

Klossek, A.; Thierbach, Steffen; Rancan, Fiorenza; Vogt, Annika; Blume-Peytavi, Ulrike; Rühl, E.

doi:10.1016/j.ejpb.2016.11.001

Cited by 15 publications

(12 citation statements)

References 32 publications

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“…Specifically, we probed proteins via the symmetric CH 3 stretch vibration (υ s CH 3 ) at 2934 cm –1 , which corresponded to a wavelength of the pump pulse of 811.2 nm. Lipids were probed by the symmetric CH 2 stretch vibration (υ s CH 2 ) at 2850 cm –1 , − requiring the wavelength of the pump pulse to be adjusted at 816.5 nm. Detection was realized by stimulated Raman loss (SRL) .…”

Section: Methodsmentioning

confidence: 99%

“…This procedure was needed to reduce artifacts and has previously been described. 23 Areas of low transmission that appear dark in optical microscopy were characterized by low SRL intensities when measured in transmission geometry. This artifact was reduced by dividing the local SRL intensity by the corresponding optical transmission, providing more realistic distributions of the probed species.…”

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confidence: 99%

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Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration

et al. 2019

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Research on topical drug delivery relies on reconstructed human skin (RHS) in addition to ex vivo human and animal skin, each with specific physiological features. Here, we compared the penetration of dexamethasone from an ethanolic hydroxyethyl cellulose gel into ex vivo human skin, murine skin, and RHS. For comprehensive insights into skin morphology and penetration enhancing mechanisms, scanning transmission X-ray microscopy (STXM), liquid chromatography tandemmass spectrometry (LC-MS/MS), and stimulated Raman spectromicroscopy (SRS) were combined. STXM offers high spatial resolution with label-free drug detection and is therefore sensitive to tissue damage. Despite differences in sample preparation and data analysis, the amounts of dexamethasone in RHS, detected and quantified by STXM and LC-MS/MS, were very similar and increased during the first 100 min of exposure. SRS revealed interactions between the gel and the stratum corneum or, more specifically, its protein and lipid structures. Similar to both types of ex vivo skin, higher protein-to-lipid ratios within the stratum corneum of RHS indicated reduced lipid amounts after 30 min of ethanol exposure. Extended ethanol exposure led to a continued reduction of lipids in the ex vivo matrixes, while protein integrity appeared to be compromised in RHS, which led to declining protein signals. In conclusion, LC-MS/MS proved the predictive capability of STXM for label-free drug detection. Combining STXM with SRS precisely dissected the penetration enhancing effects of ethanol. Further studies on topical drug delivery should consider the potential of these complementary techniques.T he limited translational success of biomedical research 1,2 demands both human cell-based 3D test tissues 3−5 and qualified concepts for predictive readouts of in vitro tests. Scanning transmission X-ray microscopy (STXM) represents a label-free and chemically selective approach to mapping drug distributions at high spatial resolution (calculated resolution down to 50 nm) in human skin. 6−9 STXM can provide new insights into cutaneous and transdermal drug delivery. However, to validate the usefulness of STXM as a quantitative technique for such studies, comparison to other, currently established analytical approaches is required. Liquid chromatography tandem-mass spectrometry (LC-MS/MS) is widely

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration

et al. 2019

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“…5B), the value S skeleton = I 1080 /(I 1130 + I 1060 ) was introduced. I 1080 was the peak intensity at 1080 cm −1 , which resulted from the gauche conformation of the skin (more disordered packing), as well as I 1130 and I 1060 were the peak intensities at 1130 cm −1 and 1060 cm −1 , which corresponded to the all trans conformation of the skin (more ordered packing) (Klossek et al, 2017). Thus, a higher S skeleton value represented a tendency towards a disordered state of skin, while lower values represented a tendency towards a more ordered state of skin (Tfayli et al, 2010) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Mechanism investigation of ethosomes transdermal permeation

Niu

Zhang

Bian

et al. 2019

International Journal of Pharmaceutics: X

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“… 100 – 102 Klossek et al. 103 have outlined the advantages of combining spontaneous RS with SRS for the investigation of lipid distributions in skin. The combination of both modalities helped to reduces the cross-sensitivity for blended Raman bands.…”

Section: Optical Modalities For Medical Diagnosticsmentioning

confidence: 99%

Looking for a perfect match: multimodal combinations of Raman spectroscopy for biomedical applications

2021

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. Raman spectroscopy has shown very promising results in medical diagnostics by providing label-free and highly specific molecular information of pathological tissue ex vivo and in vivo . Nevertheless, the high specificity of Raman spectroscopy comes at a price, i.e., low acquisition rate, no direct access to depth information, and limited sampling areas. However, a similar case regarding advantages and disadvantages can also be made for other highly regarded optical modalities, such as optical coherence tomography, autofluorescence imaging and fluorescence spectroscopy, fluorescence lifetime microscopy, second-harmonic generation, and others. While in these modalities the acquisition speed is significantly higher, they have no or only limited molecular specificity and are only sensitive to a small group of molecules. It can be safely stated that a single modality provides only a limited view on a specific aspect of a biological specimen and cannot assess the entire complexity of a sample. To solve this issue, multimodal optical systems, which combine different optical modalities tailored to a particular need, become more and more common in translational research and will be indispensable diagnostic tools in clinical pathology in the near future. These systems can assess different and partially complementary aspects of a sample and provide a distinct set of independent biomarkers. Here, we want to give an overview on the development of multimodal systems that use RS in combination with other optical modalities to improve the diagnostic performance.

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Studies for improved understanding of lipid distributions in human skin by combining stimulated and spontaneous Raman microscopy

Cited by 15 publications

References 32 publications

Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration

Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration

Mechanism investigation of ethosomes transdermal permeation

Looking for a perfect match: multimodal combinations of Raman spectroscopy for biomedical applications

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