In the present study, we investigate the stick-slip phenomenon and friction behaviour of industrial ball bearing linear guides to get some precise and comprehensive data on this matter. As friction influences some important characteristics of machine tools and the provided data from the manufacturer's catalogue do not cover all the details of various working conditions, it is essential to investigate it thoroughly. Translational guides with various preloads and under different external loads and running velocities are experimentally investigated. Afterwards, to provide a more practical condition, a slide is mounted on four carriages, and the same experiments are conducted. Finally, the friction force-time diagrams and also the friction coefficients are compared and evaluated for different working conditions.