Studie der Indolderivate in Rosenkohl (Brassica oleracea var. gemmifera)

Hormonal Relations in the Phototropic Response IV. Light-Induced Changes of Endogenous Auxins in the Coleoptile

“….\s.corhigen is inactive in the Avieii(l sectioni test all(l breaks dow\-n in the ammlioniiacal chromatographic svstenl to several biologically active compounds (15) in these resl)ects it dliffers fromii 1'. 'lle l)el)ti(les.…”

Indole Auxins

“…Ascorbigen was reported inactive in the oat cylinder test; but alkaline hydrolysis liberated IAA and indole-3-carboxylic acid whilst acid hydrolysis yielded ascorbic acid (161). Chroma tography of ascorbigen in the ammoniacal solvents widely used in indole auxin research caused complete breakdown to give physiologically active compounds, including two that had RF'S similar to IAA and IAN (107). It was considered likely that the interconvertibility obtained by repeated chromatography of cabbage extracts in ammoniacal solvents [Housley & Bentley (91)] was explicable on this basis [Kutacek et at.…”

“…Ascorbigen undergoes alkaline hydrolysis to give small yields of IAA (160,161), and IAA is also formed in ammoniacal solvents during chromatography (107). Ascorbigen is also rapidly degraded after being infused into leaves of cabbage and kohlrabi, but IAA was not detected (110).…”

“…were noted using as cor big en and ammoniacal solvents which also gave auxins, one of which appeared in the IAN zone (107) , although the fact that the precursor is active (91) and ascorbigen is inactive (161) in the oat cylinder test must not be overlooked. A similar hydrophilic complex from cabbage was also partially purified by countercurrent distribution, and its breakdown to IAN verified (156).…”

“…Tryptamine has been isolated from Acacia (221), and has been de tected in tomato (219, 220) and maize (86). The indole auxin precursor ascorbigen (or a bound form of ascorbic acid) has been extracted from various Brassicae (71,107,111,127,144,158,159,172,182,209), Citrus (7) and members of the family Papilionaceae (10).…”