“…The expanded concepts were initially ordered by the course in which students were expected to encounter the concepts. To verify and refine the initial order of concepts, literature on student understanding of acid-base chemistry was explored in depth from the literature on high school-and college-level mastery (Banerjee, 1991;Watters and Watters, 2006;Lin and Chiu, 2007;Orgill and Sutherland, 2008;Cartrette and Mayo, 2011;McClary and Talanquer, 2011;Cooper et al, 2016;Pan and Henriques, 2015;Stoyanovich et al, 2015;Romine et al, 2016;Tümay, 2016;); further guidelines from professional societies (ACS, n.d.; ASBMB, n.d.; NRC, 2013); and common biochemistry course texts (Voet and Voet, 2004;Berg et al, 2007;Nelson et al, 2013). Evidence supporting the inclusion of higher-level content was in part retrieved from biochemistry textbooks by searching the table of contents, index, and glossary for key phrases (e.g., acid, base, pH, pK a ) and consolidating knowledge expected of students as it pertains to acid-base chemistry.…”