Strukturver�nderungen am ?Murein-Sacculus? von Spirillum serpens nach Einwirkung von Uranylacetat

Preusser, H.-J.

doi:10.1007/bf00413874

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…Concerning the arrangement of macromolecules, Verwer and Nanninga (16) reported that the peptidoglycan in the cell wall of Bacillus subtilis is arranged tangentially to the long axis of this rodshaped bacterium. Such an arrangement was also suggested in Escherichia coli (17) and Spirillum serpens (11). We have presented evidence that in Staphylococcus the macromolecules are arranged in a concentric circular fashion in the newly generated cross wall (3).…”

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confidence: 57%

Arrangement of peptidoglycan in the cell wall of Staphylococcus spp

Amako¹,

Umeda²,

Murata³

1982

J Bacteriol

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The arrangement of peptidoglycan in the cell wall of Staphylococcus was observed with the newly developed freeze-fracture technique, using n-octanol instead of water as the freezing medium. The replica of the trichloroacetic acidextracted cell wall (TCA-wall) showed two areas. One of them has a concentric circular structure, a characteristic surface structure of the staphylococcal cell wall, and the other showed an irregular and rough surface. The chemical analysis of the wall revealed that the TCA-wall consisted of mostly peptidoglycan. By digesting the TCA-wall with lysozyme, the circular structures were greatly disturbed, and they disappeared after 60 min of treatment. From these observations it can be expected that the peptidoglycan is arranged in a concentric circular manner in the newly generated cell wall of Staphylococcus.

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confidence: 57%

Arrangement of peptidoglycan in the cell wall of Staphylococcus spp

Amako¹,

Umeda²,

Murata³

1982

J Bacteriol

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“…An interesting alteration of the appearance of the highly purified sacculi of A. serpens was reported by Preusser (147) to occur upon treatment with 0.012 M uranyl acetate at pH 4.5. Viewed by electron microscopy, a progressive contraction occurred along the length of the sacculi, eventually resulting in the appearance of electron-dense rings and separate, ringshaped sacculus segments. Contraction of the diameter of the sacculi was quite small compared with that for the length.…”

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confidence: 81%

Biology of the chemoheterotrophic spirilla.

Krieg¹

1976

Bacteriological Reviews

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“…On the basis of chemical determinations and thickness measurements, the murein layer of E. coli has long been considered to be extremely thin, probably monolayered (1,6,16,22,25). This view has been challenged by recent observations.…”

Section: Discussionmentioning

confidence: 99%

“…After this layer had been defined as one bag-shaped macromolecule (the murein sacculus [28]), electron microscopic estimates for the thickness of this layer (2.0 to 3.0 nm [6,22]) led to the idea of a monolayer (25), which seemed to be confirmed by the determination of the number of disaccharide units per sacculus (1). To provide for the safe extension of such a monolayer, the notion of "make before break" has been introduced; i.e., new glycan chains are first inserted by cross-linking to existing peptidoglycan before old cross-links are broken.…”

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confidence: 99%