“…Structural methods are useful in the characterization of proteins lacking a characteristic biological property, and have the advantage that alterations during isolation are more likely to affect the conformation than the primary structure. a-Crystallin, which comprises about a third of the soluble lens proteins and is closely related to the insoluble protein albuminoid (Woods & Burky, 1927;Thomann, 1962;Ruttenberg, 1965;Waley, 1965b), contains 1 thiol group/sub-unit (Waley, 1965a). This feature has now been utilized in comparisons of a-crystallin with other lens proteins, and forms the basis for a chemical method of recognizing oc-crystallin.…”