Structures, physico-chemical properties, production and (potential) applications of sucrose-derived α-d-glucans synthesized by glucansucrases

Abstract

Structures, physico-chemical properties, production and (potential) applications of sucrosederived α-d-glucans synthesized by glucansucrases Li,

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

“…Both GSs and BRSs are classified in the family 70 of glycoside hydrolases (GH) [ 22 ]. They are naturally very efficient transglucosylases and do not require expensive nucleotide-activated sugars (NDP-sugar) as substrate for the synthesis of very high molar mass polymers (from 10 3 to 10 9 g/mol) [ 7 , 20 , 23 , 24 , 25 , 26 ]. In addition, they are rather promiscuous and catalyze transglucosylation from sucrose onto a broad range of acceptors (sugars or unnatural hydroxylated molecules), the recognition and efficiency of the reaction being dependent on the enzyme selectivity [ 26 , 27 ].…”

The importance of carbon and nitrogen sources on exopolysaccharide synthesis by lactic acid bacteria and their industrial importance

“…The structure, i.e., the degree of polymerization, branching, and proportion of α (1→3) and α (1→6) linkages, varies with the organism and the type of enzyme involved in the production (Boddapati, et al, 2020). Among the mutan-producing strains reported in the literature are Streptococcus mutans 6715, Streptococcus salivarius HHT, and the mutant strains Leuconostoc mesenteroides NRRL B1118, Lactobacillus reuteri ML1, and Leuconostoc mesenteroides NRRL B-1355 (Li et al, 2020). Reports in the literature indicate that mutan solubility in water can be achieved by structure modification such as carboxymethylation, one of the effective chemical modifications reported to enhance solubility, decrease viscosity, and improve the bioactive properties of glucan polymers (Boddapati et al, 2020).…”

Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses

“… 28 , 29 Moreover, GH70 enzymes can synthesize α-glucans, which vary in terms of size, glycosidic linkages, and the degree and arrangement of branching. 30 In this study, to ascertain the functional chemical structure of NTM048 EPS to stimulate IgA production, we cloned GH70 enzymes from strain NTM048 and synthesized α-glucans, which are a major component of NTM048 EPS. We also cloned GH70 enzymes from L. mesenteroides strain JCM6124, which is the type strain of L. mesenteroides harboring lower-IgA inducing activity than strain NTM048.…”